Effect of γ-aminobutyric acid on synaptic transmission and long-term potentiation in rat superior cervical ganglion

González‐Burgos, Guillermo; Biali, Fabian I; Siri, Leonardo C. Nicola; Cardinali, Daniel P.

doi:10.1016/s0006-8993(09)90002-6

Cited by 15 publications

(8 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Taking into account the known inhibitory effect of GABA on ganglionic transmission (Adams and Brown, 1975 ; González-Burgos et al, 1994 ) we explored whether GABAergic preganglionic varicosities could store another inhibitory cotransmitter such as mENK (Zhang et al, 1995 ). In the double immunostaining for GAD67 and mENK, we found that most of the GAD67-containing varicosities did not contain mENK (93 ± 4%; Figures 5A,B ).…”

Section: Resultsmentioning

confidence: 99%

Segregation of Acetylcholine and GABA in the Rat Superior Cervical Ganglia: Functional Correlation

Elinos

Rodríguez

Martínez

et al. 2016

Front. Cell. Neurosci.

View full text Add to dashboard Cite

Sympathetic neurons have the capability to segregate their neurotransmitters (NTs) and co-transmitters to separate varicosities of single axons; furthermore, in culture, these neurons can even segregate classical transmitters. In vivo sympathetic neurons employ acetylcholine (ACh) and other classical NTs such as gamma aminobutyric acid (GABA). Herein, we explore whether these neurons in vivo segregate these classical NTs in the superior cervical ganglia of the rat. We determined the topographical distribution of GABAergic varicosities, somatic GABAA receptor, as well as the regional distribution of the segregation of ACh and GABA. We evaluated possible regional differences in efficacy of ganglionic synaptic transmission, in the sensitivity of GABAA receptor to GABA and to the competitive antagonist picrotoxin (PTX). We found that sympathetic preganglionic neurons in vivo do segregate ACh and GABA. GABAergic varicosities and GABAA receptor expression showed a rostro-caudal gradient along ganglia; in contrast, segregation exhibited a caudo-rostral gradient. These uneven regional distributions in expression of GABA, GABAA receptors, and level of segregation correlate with stronger synaptic transmission found in the caudal region. Accordingly, GABAA receptors of rostral region showed larger sensitivity to GABA and PTX. These results suggest the presence of different types of GABAA receptors in each region that result in a different regional levels of endogenous GABA inhibition. Finally, we discuss a possible correlation of these different levels of GABA modulation and the function of the target organs innervated by rostral and caudal ganglionic neurons.

show abstract

Section: Resultsmentioning

confidence: 99%

Segregation of Acetylcholine and GABA in the Rat Superior Cervical Ganglia: Functional Correlation

Elinos

Rodríguez

Martínez

et al. 2016

Front. Cell. Neurosci.

View full text Add to dashboard Cite

show abstract

“…Recombinant GABA B Receptors Fail to Function when Expressed in Ganglion Neurons-The presence of functional GABA B receptors in SCG neurons (28,29,30) and dorsal root ganglion neurons (DRG) (31) has been reported extensively in recent years (3,32). To examine the activity of recombinant GABA B receptors in ganglion neurons, the inhibition of Ca 2ϩ channel currents in response to the GABA B receptor agonist L-baclofen was studied electrophysiologically.…”

Section: Gaba B Receptors Fail To Reach the Cell Surface In Heterologmentioning

confidence: 99%

Intracellular Retention of Recombinant GABABReceptors

Couve¹,

Filippov²,

Connolly³

et al. 1998

Journal of Biological Chemistry

186

136

View full text Add to dashboard Cite

␥-Aminobutyric acid type B (GABA B ) receptors mediate the transmission of slow and prolonged inhibitory signals in the central nervous system. Two splice variants of GABA B receptors, GABA B R1a and GABA B R1b, were recently cloned from a mouse cortical and cerebellar cDNA library. As predicted, these receptors belong to the G protein-coupled receptor superfamily. We have used epitope-tagged versions of GABA B R1a receptors to study the cellular distribution of these proteins in a variety of non-neuronal and neuronal cell types. Here we report that recombinant GABA B receptors fail to reach the cell surface when expressed in heterologous systems and are retained in the endoplasmic reticulum when introduced into COS cells. In addition, we prove that recombinant GABA B receptors are excluded from the cell surface when overexpressed in ganglion neurons and we further demonstrate that they fail to activate in superior cervical ganglion neurons. Together our observations suggest that recombinant GABA B receptors require additional information for functional targeting to the plasma membrane.

show abstract

“…The main classical transmitter of the ganglionic synapse in the SCG is acetylcholine (ACh), which can be colocalized with other classical transmitters, such as GABA [26–30]. The modulatory effect of GABA on ganglionic cholinergic transmission has been described previously [30–33]. GABA acting on GABA-A and GABA-B receptors inhibits ganglionic transmission, and acting on GABA-A receptors also obstructs the occurrence of LTP in the rat SCG [33, 34].…”

Section: Introductionmentioning

confidence: 99%

Ganglionic Long-Term Potentiation in Prehypertensive and Hypertensive Stages of Spontaneously Hypertensive Rats Depends on GABA Modulation

2019

View full text Add to dashboard Cite

The sympathetic nervous system (SNS) regulates body functions in normal and pathological conditions and is characterized by the presence of a neuroplastic phenomenon, termed ganglionic long-term potentiation (gLTP). In hypertension, either in spontaneously hypertensive rats (SHR) or in humans, sympathetic hyperfunction, such as elevated SNS outflow and changes in synaptic plasticity have been described. Because enhanced SNS outflow is detected in the hypertensive stage and, more importantly, in the prehypertensive phase of SHR, here we explored whether synaptic plasticity, particularly gLTP, was modified in the superior cervical ganglia (SCG) of prehypertensive SHR. Furthermore, considering that GABA modulates sympathetic synaptic transmission and gLTP in Wistar rats, we studied whether GABA might modulate gLTP expression in SHR. We characterized gLTP in the SCG of young prehypertensive 6-week-old (wo) and adult hypertensive (12 wo) SHR and in the SCG of Wistar Kyoto (WKy) normotensive control rats of the same ages. We found that gLTP was expressed in 6 wo SHR, but not in 12 wo rats. By contrast, in WKy, gLTP was expressed in 12 wo, but not in 6 wo rats. We also found that gLTP depends on GABA modulation, as blockade of GABA-A subtype receptors with its antagonist bicuculline unmasked gLTP expression in adult SHR and young WKy. We propose that (1) activity-dependent changes in synaptic efficacy are altered not only during hypertension but also before its onset and (2) GABA may play a modulatory role in the changes in synaptic plasticity in SHR, because the blockade of GABA-A receptors unmasked the expression of gLTP. These early changes in neuroplasticity and GABA modulation of gLTP could be part of the sympathetic hyperfunction observed in hypertension.

show abstract

Effect of γ-aminobutyric acid on synaptic transmission and long-term potentiation in rat superior cervical ganglion

Cited by 15 publications

References 33 publications

Segregation of Acetylcholine and GABA in the Rat Superior Cervical Ganglia: Functional Correlation

Segregation of Acetylcholine and GABA in the Rat Superior Cervical Ganglia: Functional Correlation

Intracellular Retention of Recombinant GABABReceptors

Ganglionic Long-Term Potentiation in Prehypertensive and Hypertensive Stages of Spontaneously Hypertensive Rats Depends on GABA Modulation

Contact Info

Product

Resources

About