Effect of β-Casein (1-28) on Proliferative Responses and Secretory Functions of Human Immunocompetent Cell Lines

Kawahara, Teppei; Katayama, Daisuke; Otani, Hajime

doi:10.1271/bbb.68.2091

Cited by 22 publications

(14 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Peptides derived from milk proteins have been shown to exert digestive and metabolic effects as well . The immunoregulatory effects of casein peptides are currently actively investigated (Kawahara et al, 2004;Otani et al, 2003).…”

Section: Discussionmentioning

confidence: 99%

Casein-hydrolyzing activity of sIgA antibodies from human milk

2005

View full text Add to dashboard Cite

During pregnancy and immediately after delivery (i.e. at the beginning of lactation), the female organism is frequently characterized by an immune status similar to that of patients with autoimmune diseases. In addition, lactation is associated with an appearance of catalytically active antibodies or abzymes (Abzs) with DNAse, RNase, ATPase, amylolitic, protein kinase and lipid kinase activities in breast milk. However, until now there were no examples of human milk Abzs with a proteolytic activity. We present the first evidence that electrophoretically and immunologically homogeneous human milk sIgAs possess a beta-casein-hydrolyzing activity different from known proteases. Abzs specifically hydrolyze both human and bovine beta-caseins but not many other proteins tested. Using different methods including in situ analysis of proteolytic activity in a gel after SDS-PAGE it was shown that the observed proteolytic activity is an intrinsic property of human milk polyclonal sIgAs. Specific inhibitors of acidic and thiol proteases demonstrated a weak effect on proteolytic activity of Abzs, while a specific inhibitor of serine proteases (AEBSF) significantly inhibited the proteolytic activity of the abzymes. The K(M) value for human casein as a substrate was estimated (7.3 microM). Our findings suggest that the immune system of clinically healthy mothers can generate IgAs with a beta-casein-specific serine protease-like activity.

show abstract

Section: Discussionmentioning

confidence: 99%

Casein-hydrolyzing activity of sIgA antibodies from human milk

2005

View full text Add to dashboard Cite

show abstract

“…34) Twenty food-protein hydrolysates (a containing casein hydrolysate) hydrolyzed with gastrointestinal proteinases have been demonstrated to exert antimicrobial and immunostimulatory activities. 35) In addition, a tryptic casein hydrolysate had an immunostimulatory action by enhancing the phagocytosis of mouse peritoneal macrophages and blood phagocytic cells.…”

Section: Discussionmentioning

confidence: 99%

Effects of a Casein Hydrolysate Prepared fromAspergillus oryzaeProtease on Adjuvant Arthritis in Rats

Hatori¹,

Ohki²,

Hirano³

et al. 2008

Bioscience, Biotechnology, and Biochemistry

View full text Add to dashboard Cite

“…Milk proteins has been identified as a rich source of biologically active peptides with immunoregulatory functions (Kawahara et al, 2004;Kitazawa et al, 2007;Rusu et al, 2010;Fiedorowicz et al, 2011). The proliferative effect of milk protein b-casein (1 to 28) was measured in human T, B and monocyte cell lines (T-cell lines HUT-78, Jurkat Clone E6-1, MOLT-4; B-cell lines BALL, KHM-1B, U266B1; monocyte cell lines U937 HL-60) by a colorimetric assay supplemented with measurements of immunoglobulin A levels in supernatants and mRNA expression of interleukin-6 (IL-6) in some of the cell lines (Kawahara et al, 2004).…”

Section: Cell-based Models Of Immune Functionmentioning

confidence: 99%

“…The proliferative effect of milk protein b-casein (1 to 28) was measured in human T, B and monocyte cell lines (T-cell lines HUT-78, Jurkat Clone E6-1, MOLT-4; B-cell lines BALL, KHM-1B, U266B1; monocyte cell lines U937 HL-60) by a colorimetric assay supplemented with measurements of immunoglobulin A levels in supernatants and mRNA expression of interleukin-6 (IL-6) in some of the cell lines (Kawahara et al, 2004). In another study, the chemotactic effect of peptides derived from enzymatic digestion of b-casein was studied in a murine macrophage-like cell line (J774-1) and in human neutrophils and T lymphocytes (Kitazawa et al, 2007).…”

Section: Cell-based Models Of Immune Functionmentioning

confidence: 99%

Cell-based models to test the effects of milk-derived bioactives

Purup

Nielsen²

2012

Animal

View full text Add to dashboard Cite

The life science industries have a strong interest in screening for novel bioactives in complex mixtures like milk and dairy products. Food bioactives are not only important for public health in general, but also have potential therapeutic applications for the treatment of a number of diseases. To identify these novel bioactives, establishment of robust screening assays is essential. The use of in vitro cell-based models for screening and testing have the advantage that several concentrations of mixtures or specific compounds can be assayed at the same time in cells from specific tissues. Primary cell cultures from target organs or established cell lines can be used to identify the most sensitive cells. In addition, a large number of transfected cell lines with very specific sensitivities have been developed. Different endpoints inherent to basal or more sophisticated cellular functions can be investigated, such as cell viability, apoptosis, migration, intracellular signalling, regulation of gene expression, morphology and metabolic alterations. The gastrointestinal tract is an obvious target for bioactive molecules delivered through milk and dairy products, because it lies at the interface between dietary components in the lumen and the internal processes of the host. Identification of bioactive factors that affects proliferation or migration of epithelial cells may have potential applications in promoting gastrointestinal health in both humans and animals. The mammary gland is another target organ of considerable interest since it has been estimated that up to 50% of all newly diagnosed breast cancers may be related to dietary factors. A large number of gastrointestinal and mammary epithelial cell lines are commercially available, but in order to study some cellular functions, primary cultures of freshly isolated cells are often preferred, as established cell lines do not always express specialised properties in culture.

show abstract

Effect of β-Casein (1-28) on Proliferative Responses and Secretory Functions of Human Immunocompetent Cell Lines

Cited by 22 publications

References 17 publications

Casein-hydrolyzing activity of sIgA antibodies from human milk

Casein-hydrolyzing activity of sIgA antibodies from human milk

Effects of a Casein Hydrolysate Prepared fromAspergillus oryzaeProtease on Adjuvant Arthritis in Rats

Cell-based models to test the effects of milk-derived bioactives

Contact Info

Product

Resources

About