Effect of α-Glucosylation on the Stability, Antioxidant Properties, Toxicity, and Neuroprotective Activity of (–)-Epigallocatechin Gallate

Gonzalez, Jose L. Ugia; Peñalver, Pablo; Morales, Juan Carlos; Plou, Francisco J.

doi:10.3389/fnut.2019.00030

Cited by 30 publications

(26 citation statements)

References 57 publications

(66 reference statements)

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“…For example, despite curcumin having promising antioxidant, anticancer, anti-inflammatory, and antimicrobial activities, it has not been released as a drug yet due to its poor bioavailability ( 292 ). Another propitious drug candidate, epigallocatechin gallate (EGCG), showed antioxidant, antihypertensive, anticancer, antimicrobial, and anti-inflammatory activity ( 293 , 294 ) but unfortunately, it has also failed to obtain drug designation due to the same reason mentioned for curcumin ( 292 ).…”

Section: Drug Discovery From Psms: Addressing the Major Challenges Tomentioning

confidence: 99%

Plants Metabolites: Possibility of Natural Therapeutics Against the COVID-19 Pandemic

et al. 2020

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COVID-19, a disease induced by SARS-CoV-2 (Severe Acute Respiratory Syndrome Coronavirus-2), has been the cause of a worldwide pandemic. Though extensive research works have been reported in recent days on the development of effective therapeutics against this global health crisis, there is still no approved therapy against SARS-CoV-2. In the present study, plant-synthesized secondary metabolites (PSMs) have been prioritized to make a review focusing on the efficacy of plant-originated therapeutics for the treatment of COVID-19. Plant metabolites are a source of countless medicinal compounds, while the diversity of multidimensional chemical structures has made them superior to treat serious diseases. Some have already been reported as promising alternative medicines and lead compounds for drug repurposing and discovery. The versatility of secondary metabolites may provide novel antibiotics to tackle MDR (Multi-Drug Resistant) microbes too. This review attempted to find out plant metabolites that have the therapeutic potential to treat a wide range of viral pathogens. The study includes the search of remedies belonging to plant families, susceptible viral candidates, antiviral assays, and the mode of therapeutic action; this attempt resulted in the collection of an enormous number of natural therapeutics that might be suggested for the treatment of COVID-19. About 219 plants from 83 families were found to have antiviral activity. Among them, 149 plants from 71 families were screened for the identification of the major plant secondary metabolites (PSMs) that might be effective for this pandemic. Our investigation revealed that the proposed plant metabolites can serve as potential anti- SARS-CoV-2 lead molecules for further optimization and drug development processes to combat COVID-19 and future pandemics caused by viruses. This review will stimulate further analysis by the scientific community and boost antiviral plant-based research followed by novel drug designing.

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Section: Drug Discovery From Psms: Addressing the Major Challenges Tomentioning

confidence: 99%

Plants Metabolites: Possibility of Natural Therapeutics Against the COVID-19 Pandemic

et al. 2020

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“…One of the main disadvantages of these substances when used in treatments is their low bioavailability, and their glycosylation, which can increase its solubility, has been proposed as a potential solution. In this context, various studies have demonstrated the interesting properties of hydroxytyrosol, vanillin and gallic acid [34][35][36], and EGCG has recently attracted attention as a potential therapeutic agent [37,38], even in its glycosylated forms [39]. In a first approximation TLC analysis of the reaction mixtures was used to detect the synthesis of glycosides from the selected acceptors, identifying positive spots for each potential glycoside ( figure S4).…”

Section: Conversion Of Bgl-1 Into Glycosynthases By Rational Designmentioning

confidence: 99%

A glucotolerant β-glucosidase from the fungus Talaromyces amestolkiae and its conversion into a glycosynthase for phenolic compounds glycosylation.

Méndez-Líter

Nieto-Domínguez

Toro

et al. 2019

Preprint

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Background: The interest for finding novel b-glucosidases that can improve the yields for the production of second-generation (2G) biofuels is still very high. One of the most desired features for these enzymes is the glucose tolerance, which allows them to act optimally under elevated glucose concentrations. Besides, there is an additional focus of attention on finding novel enzymatic alternatives for glycoside synthesis, for which a mutated version of glycosidases, named glycosynthases, have gained much interest in recent years. Results: In this work, a glucotolerant β-glucosidase (BGL-1) from the ascomycete fungus Talaromyces amestolkiae has been heterologously expressed in Pichia pastoris , purified, and characterized. The production of the enzyme in the yeast was very high, reaching 75 U/mL, and it allowed purification in just one step with a yield of 80%. Although the enzyme showed good efficiency on p NPG ( K m =3.36 ± 0.7 mM, k cat =898.31 s -1 ), the activity detected on cellooligosaccharides, the natural substrates of the enzyme, was much lower, which could limit its exploitation in lignocellulose degradation processes. Interestingly, when examining the substrate specificity of BGL-1, it showed to be more active on sophorose, the b-1,2 disaccharide of glucose, than on cellobiose. Besides, the transglycosylation profile of BGL-1 was examined, and, for expanding its synthetic capacities, it was converted into a glycosynthase. The mutant enzyme, named BGL-1-E521G, was able to use α-D-glucosyl-fluoride as donor in glycosylation reactions, and synthesized glucosylated derivatives of different p NP-sugars in a regioselective manner, as well as of some phenolic compounds of industrial interest, such as epigallocatechin gallate (EGCG). Conclusions: In this work, we report the characterization of a novel glucotolerant 1,2-β-glucosidase, which also has a considerable activity on 1,4-β-glucosyl bonds, that has been cloned in P. pastoris , produced, purified and characterized. In addition, the enzyme was converted into an efficient glycosynthase, which can transfer glucose molecules to glucose to a diversity of acceptors for obtaining compounds of interest. The remarkable capacities of BGL-1 and its glycosynthase mutant, both in hydrolysis or in synthesis, suggest that it could be an enzyme with applications for industrial processes.

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“…On these bases, polyphenols can be categorized as flavonoids (including anthocyanins, flavanes, flavonols, isoflavones, and flavan-3ols), phenolic acids, polyphenolic amides, and other polyphenol compounds. Polyphenols have been widely researched for their various health benefits, in particular their good antioxidant and anti-inflammatory properties [65,[67][68][69]. With their beneficial effects on various chronic diseases and on bacterial and viral infections, the properties of polyphenols can be a quick, healthy and cost-effective solution in several cellular and molecular pathways, contributing to a better immunity of the body [69].…”

Section: Polyphenols As Natural Products Of Nutritional Interestmentioning

confidence: 99%

Impact of Zinc, Glutathione, and Polyphenols as Antioxidants in the Immune Response against SARS-CoV-2

et al. 2021

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SARS-CoV-2, the coronavirus triggering the disease COVID-19, has a catastrophic health and socioeconomic impact at a global scale. Three key factors contribute to the pathogenesis of COVID-19: excessive inflammation, immune system depression/inhibition, and a set of proinflammatory cytokines. Common to these factors, a central function of oxidative stress has been highlighted. A diversity of clinical trials focused predominantly on antioxidants are being implemented as potential therapies for COVID-19. In this study, we look at the role of zinc, glutathione, and polyphenols, as key antioxidants of possible medicinal or nutritional significance, and examine their role in the antiviral immune response induced by SARS-Cov-2. An unresolved question is why some people experience chronic COVID and others do not. Understanding the relationship between SARS-CoV-2 and the immune system, as well as the role of defective immune responses to disease development, would be essential to recognize the pathogenesis of COVID-19, the risk factors that affect the harmful consequences of the disease, and the rational design of successful therapies and vaccinations. We expect that our research will provide a novel perspective that contributes to the design of clinical or nutritional targets for the prevention of this pandemic.

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Effect of α-Glucosylation on the Stability, Antioxidant Properties, Toxicity, and Neuroprotective Activity of (–)-Epigallocatechin Gallate

Cited by 30 publications

References 57 publications

Plants Metabolites: Possibility of Natural Therapeutics Against the COVID-19 Pandemic

Plants Metabolites: Possibility of Natural Therapeutics Against the COVID-19 Pandemic

A glucotolerant β-glucosidase from the fungus Talaromyces amestolkiae and its conversion into a glycosynthase for phenolic compounds glycosylation.

Impact of Zinc, Glutathione, and Polyphenols as Antioxidants in the Immune Response against SARS-CoV-2

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