Effect of Zn2+ and Mg2+ on alkaline phosphatase from human placenta and intestine.

Sugiura, Masahiro; Hirano, Kazuyuki; Iino, Shiro; Suzuki, Hiroshi; Oda, Tatsuya

doi:10.1248/cpb.25.653

Cited by 9 publications

(2 citation statements)

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“…Die alkalische Phosphatase aus E. coli besitzt ein Molekulargewicht von 89 000 und enthfilt 4 g Atome Zink pro Mol [2,3]. Das Metall spielt dabei nicht nur eine Rolle fiir die katalytische Aktivit/it des Enzymmolekt~ls, sondern dient auch zur Stabilisierung der Terti~r-struktur [15,55]. Friihere Studien zeigten bereits, dab zwei Zn-Atome in lockerer Bindung ft~r die katalytische Aktivit/it essentiell sind [38] und dab zwei zusfitzliche Atome an Zink in starker Bindung die Proteinstruktur stabilisieren [53].…”

Section: Bei Den Jungenunclassified

Experimentelle Untersuchungen zur Diagnose von marginalem Zinkmangel

Roth

Kirchgeßner

1979

Res. Exp. Med.

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Experimental Studies on the Diagnosis of Marginal Zinc DeficiencySummary. A hundred and twelve male Sprague-Dawley rats with an average live weight of 37 g were depleted for 14 days with a Zn-deficient diet of 1.3 mg Zn/kg. Thereafter, they were divided into 16 groups of 7 animals each. For another 14 days, two groups each were given diets containing 1.3, 4, 6, 8, 10, 12, 20 and 100ppm Zn. Then one of each of these groups were sacrificed, while the other received a Zn injection of 0.8 mg Zn/animal. Three days later, these 8 groups were also sacrificed in order to test the effect of Zn injection on several parameters of Zn supply.1. The Zn requirement for an optimum growth of the young rats was reached at 12 ppm. 2. The serum and bone contents increased in response to Zn injection up to the dietary supply of 20 ppm Zn; the Zn content of the testes was not affected. 3. The activity of the alkaline phosphatase in serum showed a significant increase after Zn injection up to those animals given 20 ppm dietary zinc and remained unchanged at 100ppm. 4. In bone and testes the activity of the alkaline phosphatase increased after Zn injection up to the groups with 8 and 10 ppm dietary Zn, respectively.The activity of the alkaline phosphatase in serum and its response to Zn injection thus represents a simple and sensitive system to assess the Zn supply status, especially the marginal supply. If an increase in the activity of the serum alkaline phosphatase can be obtained by Zn injection, a more or less pronounced Zn undernutrition, depending upon the observed activity increase, is indicated because no increase in the activity could be effected by additional Zn doses when the Zn status was optimal. 1. Der Zn-Bedarf ffir ein optimales Wachstum der jungen Ratten war bei 12 ppm erreicht. 2. Serum-und Knochen-Zn-Gehalte erh6hten sich nach einer Zn-Injektion bis zu einem Di~it-Zn-Gehalt von 20ppm und blieben im Hoden bei allen Gruppen unbeeinflugt. 3. Die Aktivitfit der alkalischen Phosphatase im Serum zeigte auf die ZnInjektion eine signifikante Aktivit~itserh6hung bis zu den Tieren mit einem Di~it-Zn-Gehalt von 20ppm. Sie blieb bei 100ppm unver~indert. 4. Im Knochen und Hoden erh6hte sich die Aktivit~it der alkalischen Phosphatase nach einer Zn-Injektion bis zu den Gruppen mit 8 bzw. 10 ppm DifitZn-Gehalt.Die Aktivit~it der alkalischen Phosphatase im Serum und deren Respons infolge einer Zn-Injektion stellt damit ein einfaches und sensitives System zur Bestimmung des Zn-Versorgungsstatus, speziell im marginalen Bereich, dar. Lfigt sich aufgrund einer Zn-Injektion eine Aktivit~itserh6hung der alkalischen Phosphatase im Serum erzielen, so mug je nach der H6he der festgestellten Aktivitfitserh6hung eine mehr oder weniger ausgeprfigte Zn-Unterversorgung vorgelegen haben, da bei einer optimalen Versorgung sich durch zusfitzliche Zn-Gaben keine Aktivit~ttserh6hung erzielen lieg.Schliisselwiirter: Zn-Mangel -Diagnose von Zn-Mangel -alkalische Phosphatase -saure Phosphatase -Zn-Injektion -Serum-Zn -Femur-ZnHoden-Zn

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Section: Bei Den Jungenunclassified

Experimentelle Untersuchungen zur Diagnose von marginalem Zinkmangel

Roth

Kirchgeßner

1979

Res. Exp. Med.

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“…L'étude des propriétés biochimiques des protéines phosphorylées de la microvillosité duodénale conduit aussi, après dissociation, à les identifier aux intermédiaires phosphorylés des monomères de la phosphatase alcaline (De Jonge et al, 19811. Sans préjuger du mécanisme de la phosphorylation de la phosphatase membranaire, on peut retenir que l'activité phosphatasique et (Dupuis et al, 1981 (Cathala et al, 1975et Harkness, 1968. Zn++ + est parfois inhibant (Sugiura et al, 1977 …”

Section: Introductionunclassified

Action des cations bivalents sur l'activité de la phosphatase alcaline des microvillosités intestinales. Influence du pH et du substrat

Tardivel¹,

Rabesona²,

L³

et al. 1987

Reprod. Nutr. Dévelop.

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pH‐stat methodology in continuous monitoring of the kinetics of hydrolysis of phosphate esters catalysed by alkaline phosphatase from human placenta: II. Kinetic aspects

Roig

Serrano

Bello

et al. 1991

Polymer International

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The adaptation of the pH‐stat to continuous monitoring of the in‐vitro hydrolase activity of alkaline phosphatase in solution, an activity which until now has only been analysed by means of spectrophotometric methods in a continuous or static state, is described. To control the reliability of the method in the monitoring of these enzymic reactions, a series of kinetics of hydrolysis of chromogenic (p‐nitrophenyl and o‐carboxyphenyl phosphates) and non‐chromogenic (ATP) substrates was carried out, comparing the results obtained via continuous spectrophotometric analysis of the corresponding phenol released or via a discontinuous technique by the phosphate produced with those results obtained via pH‐stat analysis of the H+ released or utilized by the enzymic reaction of hydrolysis. It can be concluded that kinetic studies carried out on the pH‐stat of in‐vitro alkaline phosphatase activity offer results analogous to those obtained for the same system through classic spectrophotometric methods, offering as well notable advantages of speed and simplicity in the kinetic assays since it is always possible to monitor the enzymic activity continuously, with chromogenic or non‐chromogenic substrates. The pH‐stat methodology affords additional information on the kinetics of action of alkaline phosphatase from placenta acting on non‐chromogenic biological substrates. In this sense, kinetic studies with the pH‐stat on the enzymic hydrolysis of ATP were begun, determining their rate versus pH profile (optimum pH at 9.1) and proposing possible ‘non‐Michaelian’ type kinetic behaviour of the enzyme in solution as deduced from the graphical analysis of the data v([ATP]).

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Effect of Zn2+ and Mg2+ on alkaline phosphatase from human placenta and intestine.

Cited by 9 publications

References 0 publications

Experimentelle Untersuchungen zur Diagnose von marginalem Zinkmangel

Experimentelle Untersuchungen zur Diagnose von marginalem Zinkmangel

Action des cations bivalents sur l'activité de la phosphatase alcaline des microvillosités intestinales. Influence du pH et du substrat

pH‐stat methodology in continuous monitoring of the kinetics of hydrolysis of phosphate esters catalysed by alkaline phosphatase from human placenta: II. Kinetic aspects

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