Effect of Zinc Oxide Nanoparticles on Physiological Activities and Gene Expression of Wastewater Nitrifying Bacteria

Phan, Duc; Pasha, Akbar; Carwile, Nikolas; Kapoor, Vikram

doi:10.1089/ees.2019.0385

Cited by 7 publications

(7 citation statements)

References 41 publications

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“…Performance deterioration is often measured by respirometry methods (specific oxygen uptake rate) and nitrogen depletion assays (ammonia-utilization rate and/or nitrite/nitrate production rate) (Phan et al, 2020a(Phan et al, , 2020b. Cellular damages are either assessed biochemically (LDH release, ROS production) (Arnaout & Gunsch, 2012;Gu et al, 2014;Wu et al, 2018;Z.…”

Section: Methods and Assays For Enp Toxicity Assessmentmentioning

confidence: 99%

“…Activities of important enzymes, such as AMO, NOR, NAR, and NIR are determined offline by taking samples from the sludge reactor, extracting the enzymes from the biomass, and determined their activities using standard assays (Li et al, 2020;. In addition, the expression levels of genes encoding these enzymes can be measured by q-PCR and provided a genetic level assessment of bioactivity changes (Phan et al, 2020a(Phan et al, , 2020b. Microbial community shift reflects the differential responses of the various microorganisms within the sludge floc and is often assessed by q-PCR and high-throughput sequencing (Yazdanbakhsh et al, 2019).…”

Section: Methods and Assays For Enp Toxicity Assessmentmentioning

confidence: 99%

“…• Heavy metals, such as copper, zinc, nickel, cadmium, and hexavalent chromium (Kim et al, 2016;Phan et al, 2020aPhan et al, , 2020bWu et al, 2018).…”

Section: Inhibitionmentioning

confidence: 99%

“…Hou et al (2013) reported that ZnO NP injections (at 5 mg/L) inhibited nitrifier respiration in activated sludge. A study by Phan et al (2020a) showed that exposure to 10 mg/L of ZnO NP for 3 or 6 h resulted in nitrification inhibition by 35% and 50%, respectively. The same study also reported decreased transcription levels of the genes involved and increased ROS production in nitrifying bacteria.…”

Section: Observations With Activated Sludge: the Effect Of Short-term Enp Injectionsmentioning

confidence: 99%

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The effects of engineered nanoparticles on nitrification during biological wastewater treatment

Xing

Harper

2021

Biotech & Bioengineering

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Technological advancements in the past few decades have made it possible to manufacture nanomaterials at a large scale, and engineered nanoparticles (ENPs) are increasingly found in consumer products, such as cosmetics, sports products, and LED displays. A large amount of these ENPs end up in wastewater and potentially impact the performance of wastewater treatment plants (WWTPs). One important function of the WWTP is nitrification, which is carried out by the actions of two groups of bacteria, ammonia-oxidizing bacteria (AOB), and nitrite-oxidizing bacteria (NOB). Since most ENPs are found to have or are designed to have antimicrobial activities, it is a legitimate concern that ENPs entering WWTPs may have negative impacts on nitrification. In this paper, the effects of ENPs on nitrification are discussed, focusing mainly on autotrophic nitrification by AOBs and NOBs. This review also covers ENP effects on anaerobic ammonium oxidation (anammox).Generally, nitrifiers in pure and mixed cultures can be inhibited by a variety of ENPs, but stress response mechanisms may attenuate toxicity. Long-term studies demonstrated that a wide range of NPs could cause severe deterioration of AOBs and/ or NOBs when the influent concentration exceeded an inhibition threshold. Proposed mechanisms include the generation of reactive oxygen species, dissolved metals, physical disruption of cell membranes, bacterial engulfment, and intracellular accumulation of ENPs. Future research needs are also discussed.

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Section: Methods and Assays For Enp Toxicity Assessmentmentioning

confidence: 99%

Section: Methods and Assays For Enp Toxicity Assessmentmentioning

confidence: 99%

“…• Heavy metals, such as copper, zinc, nickel, cadmium, and hexavalent chromium (Kim et al, 2016;Phan et al, 2020aPhan et al, , 2020bWu et al, 2018).…”

Section: Inhibitionmentioning

confidence: 99%

Section: Observations With Activated Sludge: the Effect Of Short-term Enp Injectionsmentioning

confidence: 99%

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The effects of engineered nanoparticles on nitrification during biological wastewater treatment

Xing

Harper

2021

Biotech & Bioengineering

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“…Long-term exposure of nanomaterials to wastewater microbial communities is subject to more variabilities than acute, short-term exposures. Some studies showed that bacterial communities tend to recover their metabolic activities and develop resistance by selecting for inhibitor-resistant cultures during long-term exposures, ,− whereas other studies showed no significant recovery and continuous deterioration in nutrient dynamics and microbial diversity. , In addition, hybridization can remarkably change nanomaterial properties which may induce unique physicochemical interactions. In this study, we showed for the first time that rGO–nZVI NHs (1:2 and 1:0.2) have significant differences from their parent nanomaterials (i.e., rGO and nZVI) in terms of microbial community structure and dynamics, system performance, and transcriptional responses.…”

Section: Significance and Environmental Implicationsmentioning

confidence: 99%

Long-Term Exposure and Effects of rGO–nZVI Nanohybrids and Their Parent Nanomaterials on Wastewater-Nitrifying Microbial Communities

Alam

Masud

Scharf

et al. 2021

Environ. Sci. Technol.

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Single nanomaterials and nanohybrids (NHs) can inhibit microbial processes in wastewater treatment, especially nitrification. While existing studies focus on short-term and acute exposures of single nanomaterials on wastewater microbial community growth and function, long-term, low-exposure, and emerging NHs need to be examined. These NHs have distinctly different physicochemical properties than their parent nanomaterials and, therefore, may exert previously unknown effects onto wastewater microbial communities. This study systematically investigated long-term [∼6 solid residence time [(SRT)] exposure effects of a widely used carbon–metal NH (rGO–nZVI = 1:2 and 1:0.2, mass ratio) and compared these effects to their single-parent nanomaterials (i.e., rGO and nZVI) in nitrifying sequencing batch reactors. nZVI and NH-dosed reactors showed relatively unaffected microbial communities compared to control, whereas rGO showed a significantly different (p = 0.022) and less diverse community. nZVI promoted a diverse community and significantly higher (p < 0.05) biomass growth under steady-state conditions. While long-term chronic exposure (10 mg·L–1) of single nanomaterials and NHs had limited impact on long-term nutrient recovery, functionally, the reactors dosed with higher iron content, that is, nZVI and rGO–nZVI (1:2), promoted faster NH4 +-N removal due to higher biomass growth and upregulation of amoA genes at the transcript level, respectively. The transmission electron microscopy images and scanning electron microscopyenergy-dispersive X-ray spectroscopy analysis revealed high incorporation of iron in nZVI-dosed biomass, which promoted higher cellular growth and a diverse community. Overall, this study shows that NHs have unique effects on microbial community growth and function that cannot be predicted from parent materials alone.

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Short-term effects of Mn2O3 nanoparticles on physiological activities and gene expression of nitrifying bacteria under low and high dissolved oxygen conditions

et al. 2020

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Effect of Zinc Oxide Nanoparticles on Physiological Activities and Gene Expression of Wastewater Nitrifying Bacteria

Cited by 7 publications

References 41 publications

The effects of engineered nanoparticles on nitrification during biological wastewater treatment

The effects of engineered nanoparticles on nitrification during biological wastewater treatment

Long-Term Exposure and Effects of rGO–nZVI Nanohybrids and Their Parent Nanomaterials on Wastewater-Nitrifying Microbial Communities

Short-term effects of Mn2O3 nanoparticles on physiological activities and gene expression of nitrifying bacteria under low and high dissolved oxygen conditions

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