Effect of zinc nanoparticles on embryo and chicken growth, and the content of zinc in tissues and faeces

Abstract

The hypothesis was that owing to their high bioavailability, zinc oxide nanoparticles (NanoZnO) can effectively replace (Zn) salts and reduce Zn excretion with faeces. The objective of this study was to investigate the effects of NanoZnO on the development of chicken embryos, the growth of broiler chickens, and Zn excretion with faeces. At day 1 of incubation, 120 eggs were randomly divided between a control group (not injected) and groups injected with a hydrocolloid of NanoZnO in increasing concentrations (50, 100, 500 mg/L). At day 19 of incubation, no differences were observed in the bodyweight, but 100 and 500 mg/L affected liver and heart weights, indicating that high levels of NanoZnO may induce differential organ development. In the subsequent experiment, 308 chickens were randomly divided into six groups. The control diet was supplemented with 55 mg Zn/kg (standard level), the 0 group received no Zn supplement, and groups fed NanoZnO received 25%, 50%, 75%, and 100% of the standard level. The 100% replacement of ZnO with NanoZnO increased the chickens’ bodyweight compared with the standard level of ZnO, but to the same level as the diet without ZnO supplementation. Furthermore, NanoZnO did not reduce the content of Zn in faeces, which was only significantly lower in the group without ZnO supplementation in comparison with other groups. The results indicate that the replacement of ZnO with NanoZnO had no negative effects on chicken growth. Compared with ZnO, NanoZnO did not reduce Zn excretion with faeces.
 Keywords: broiler, development, excretion, mineral, nanonutrition

Calcium Carbonate Nanoparticles—Toxicity and Effect of In Ovo Inoculation on Chicken Embryo Development, Broiler Performance and Bone Status

“…After cooling, selected organs were collected (liver, muscle, and both femurs and tibias), weighed, and stored at −80 °C. IOI procedure and the minimum necessary sample size estimation were performed with minor modifications as described by Lukasiewicz et al and Pineda et al [ 29 , 30 ].…”

Effect of Zinc Oxide Nanoparticles in Drinking Water on Growth Rate, Biochemical Parameters, and Intestinal Histology of Broilers

“…Te same results showed that nano-Znimproved the broiler weight and immune system organs [5,25]. Tis result does not agree with Hasan [26] and Łukasiewicz et al [1] who showed that replacing ZnO withZnO nanoparticles had no benefcial efect on broiler performance.Numerous studies on broilers have shown thatnano-Zn has higher bioavailability compared to the traditionalZn source, suggesting that measuring the concentrationof minerals in organs could allow a more accurateassessment of nanoparticle bioavailability [23,24,27].…”

“…Zinc plays an important role in many biological activities that occur in the body [1] and is considered an important nutrient for poultry, and Zn is commonly added as a supplement to all formulated poultry diets at a rate of 0.012-0.018% [2]. Te biological activity of Zn is required for growth and skeletal development [3].…”

“…According to El-Shenawy et al [13], there is a scarcity of data that explain the impact of nano-Zn on poultry production and health. On the other hand, Łukasiewicz et al [1] noted that the replacement of zinc oxide with ZnO nanoparticles had no signifcant efect on broiler performance. According to Kumar et al [14], broiler hens' growth performance and gut shape can all be improved by adding Zn to the diet.…”