Effect of zeta potential of cationic liposomes containing cationic cholesterol derivatives on gene transfection

Takeuchi, K.-I.; Ishihara, Minoru; Kawaura, Chiyo; Noji, Masahide; Furuno, Tadahide; Nakanishi, Mamoru

doi:10.1016/s0014-5793(96)01136-2

Cited by 102 publications

(90 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It is already known that delivery to parenchymal cells in vivo is favored by small liposomes. 6 For most vectors, high efficiency of transfection correlates with a large excess of cationic charges, [38][39][40] however, this large excess in vivo facilitates non-specific interactions with many undesired elements such as extracellular matrix and negatively charged serum components. Taking this into account, the first aim of this work was to optimize the size and the zeta potential of AF-lipoplexes for maximum uptake by the liver after systemic administration.…”

Section: Discussionmentioning

confidence: 99%

Increased receptor-mediated gene delivery to the liver by protamine-enhanced-asialofetuin-lipoplexes

2003

View full text Add to dashboard Cite

A novel lipidic vector composed of DOTAP/Chol liposomes, asialofetuin (AF), protamine sulfate and DNA has been developed. The resulting protamine-AF-lipoplexes improved significantly the levels of gene expression in cultured cells and in the liver upon i.v. administration. Lipoplexes containing the optimal amount of AF (1 mg/mg DNA) showed a 16-fold higher transfection activity in HepG2 cells than nontargeted (plain) complexes. The uptake by cells having asialoglycoprotein receptors (ASGPr) on their plasma membrane was decreased by the addition of free AF, indicating that AF-lipoplexes were taken up specifically by cells via ASGPr-mediated endocytosis. Results from transfections performed in cells defective in ASGPr, ie HeLa cells, confirmed this mechanism. By addition of the condensing peptide, protamine sulfate, smaller complexes were obtained, which enhanced even more the uptake of AFcomplexes in HepG2 cells and in the liver. The optimal amount of protamine was 0.4 mg/mg DNA, and gene expression was about 5-fold over that obtained with AF-lipoplexes in the absence of the peptide, and 75-fold higher than that with plain conventional lipoplexes. Protamine-AF-lipoplexes increased by a factor of 12 luciferase gene expression in the liver of mice administered systemically via the tail vein, compared to plain complexes. In summary, our findings extend the scope of previous studies where AF-lipoplexes were used to introduce DNA into hepatocytes. The combination of targeting and protamine condensation obviated the need for partial hepatectomy, commonly required to obtain efficient gene delivery in this organ. Since protamine sulfate has been proven to be non-toxic in humans, the novel liverspecific vector described here may be useful for the delivery of clinically important genes to this organ.

show abstract

Section: Discussionmentioning

confidence: 99%

Increased receptor-mediated gene delivery to the liver by protamine-enhanced-asialofetuin-lipoplexes

2003

View full text Add to dashboard Cite

show abstract

“…Such a polycationic amphiphile should not only have good DNA-condensing properties but should also confer increased resistance to degradation to liposomes in a serum-containing environment. 18,19 The synthesized crude Arg-Chol as well as its TLCand HPLC-purified preparations appear to have a similar capability to cause the transfer of reporter plasmid DNA (see Materials and Methods); all three were found suitable for transfection studies.…”

Section: Discussionmentioning

confidence: 99%

A new cholesterol derivative suitable for transfecting certain type of cells in the presence of 10% serum

et al. 2000

View full text Add to dashboard Cite

We developed a new cationic lipid suitable for use as a DNA carrier in the presence of 10% sera. The novel compound (abbreviated as Arg-Chol) contains cholesterol and a dipeptide consisting of glycine and sterically protected arginine. The efficiency of reporter gene transfection using liposomes based on this new reagent was compared with that of liposomes made with other cationic derivatives of cholesterol. Lipoplexes formulated with the newly synthesized lipid mediate in vitro transfection of B16(F10) murine melanoma cells in the presence of 10% sera more efficiently than in other cell lines and compared with other cholesterol derivatives studied. Cancer Gene Therapy (2000) 7, 513-520

show abstract

“…For most vectors, high efficiency in vitro transfection correlates with a global excess of cationic charges. 51,52 However, this excess of cationic charges could in vivo facilitate nonspecific interaction with many undesired elements such as extracellular matrix, and negatively charged serum components, such as albumin. This latter assumption seems supported by the low in vitro transfection activity of highly cationic DNA vector/particles in the presence of serum 53 ( Figure 7).…”

Section: Dna-condensing Peptides For Transfectionmentioning

confidence: 99%

Synthetic DNA-compacting peptides derived from human sequence enhance cationic lipid-mediated gene transfer in vitro and in vivo

et al. 1999

View full text Add to dashboard Cite

Cationic lipids can deliver genes efficiently in vitro, but are with classical DNA/lipid lipoplexes, and particularly confer generally inhibited by the presence of serum, and their the capacity to transfect in the presence of serum. This efficiency in vivo is much lower than in vitro. An attractive acquisition of serum resistance is cell type-independent, strategy is to induce strong DNA compaction by its associand observed with all four lipopolyamines tested and polyation with proteins, before addition of lipids. However the ethylenimine. Precompacting DNA with a histone H1-use of whole proteins might present both production and derived peptide enhances cationic lipid RPR 115335-immunological limitations. We have devised a system in mediated gene transfer in an in vivo model of Lewis lung which DNA is associated with short peptides derived from carcinoma. Apart from their use in peptide-DNA-lipid human histone or protamine, before the addition of a catassociation, such peptides could be useful as part of chimionic lipid or polymer. Peptides strongly associating with eric gene delivery vectors presenting a DNA-binding moi-DNA confer to such peptide-DNA-lipid particles an ety that can be easily associated with other functional enhanced in vitro transfection efficiency over that observed domains.

show abstract

Effect of zeta potential of cationic liposomes containing cationic cholesterol derivatives on gene transfection

Cited by 102 publications

References 16 publications

Increased receptor-mediated gene delivery to the liver by protamine-enhanced-asialofetuin-lipoplexes

Increased receptor-mediated gene delivery to the liver by protamine-enhanced-asialofetuin-lipoplexes

A new cholesterol derivative suitable for transfecting certain type of cells in the presence of 10% serum

Synthetic DNA-compacting peptides derived from human sequence enhance cationic lipid-mediated gene transfer in vitro and in vivo

Contact Info

Product

Resources

About