Effect of xanthohumol on melanogenesis in B16 melanoma cells

Abstract

Xanthohumol (XH), the principal prenylflavonoid of the hop plant (Humulus lupulus L.), dose-dependently inhibited isobutylmethylxanthine (IBMX)-induced melanogenesis in B16 melanoma cells, with little cytotoxicity at the effective concentrations. Decreased melanin content was accompanied by reduced tyrosinase enzyme activity, protein and mRNA expression. The levels of tyrosinase-related protein 1 and 2 mRNAs were decreased by XH. XH also inhibited α-melanocyte stimulating hormone-or forskolin-induced increases in melanogenesis, suggesting an action on the cAMP-dependent melanogenic pathway. XH downregulated the protein and mRNA expression of microphthalmia-associated transcription factor (MITF), a master transcriptional regulator of key melanogenic enzymes. These results suggest that XH might act as a hypo-pigmenting agent through the downregulation of MITF in the cAMP-dependent melanogenic pathway.

FermentedViola mandshuricaInhibits Melanogenesis in B16 Melanoma Cells

“…CEFV was found to inhibit mRNA and protein levels of MITF in a dose-dependent manner, MITF is known as a major transcription factor in the regulation of tyrosinase expression, and thus plays a critical role in melanin biosynthesis. [8][9][10][11] Our results are consistent with the findings of a recent report 37) that xanthohumol (XH) significantly decreased mRNA and protein levels of MITF, suggesting that XH inhibits MITF transcription.…”

Saponified Evening Primrose Oil Reduces Melanogenesis in B16 Melanoma Cells and Reduces UV‐Induced Skin Pigmentation in Humans

“…Moreover, aleosin, licorice extract, ascorbic acid and soy proteins have been used to interrupt the process of melanogenesis [6]. We also recently reported that xanthohumol, scoparone and Angelica gigas extract exerted a potent inhibitory effect against melanogenesis in B16 melanoma cells [9][10][11]. During screening for new skin-whitening agents from natural sources, we found that the saponification product of evening primrose oil (sap-EPO) effectively whitened skin that had been subjected to UV-induced hyperpigmentation.…”

Inhibitory Effects of Bakuchiol, Bavachin, and Isobavachalcone Isolated fromPiper longumon Melanin Production in B16 Mouse Melanoma Cells

“…23) In addition, xanthohumol, a principal prenylflavonoid, has been reported to have antimelanogenesis effects by acting on cAMP-dependent signal transduction in melanocytes. 24) Since 1-3 have structures that are similar to these compounds, it is possible that their mechanisms of action against melanin biosynthesis are the same. Further biological activities of 1-3 are now being investigated.…”