Effect of X-rays on structural, physicochemical and functional properties of gluten protein

Abstract

The gluten protein was exposed to the X-ray radiations for different time range, comprising 1 and 3 seconds. The objective of this study was to determine the effect of x-ray radiations on the physicochemical properties of gluten protein. Different functional properties of proteins like water and oil holding capacities, protein solubility, emulsification activity, and stability index, foaming action and stability, water solubility, protein, and moisture content, along with SDS PAGE, FTIR, Xeta potential net charge was carried out to evaluate the effect of X-ray radiation on gluten protein. Results showed that the enhancement of water holding capacity up to 38.12%, as well as oil holding capacity up to 35% could be seen, whereas a significant decrease in emulsification activity and stability index, foaming capacity and stability, even protein content could be observed in treated samples. The net charge on protein in water solution was found to increase towards the positive side. The structure of the protein remained unchanged based on no change was observed in SDS PAGE electrograph, FTIR secondary structure region. Hence, X-ray treatment can be a possible way to alter the protein structure for “tailor-made applications” in food industries.

Reduction of food histamine in white wine by Bromelain, Quercetin and Ascorbic acid

“…the normal wine and the 200 mg/L bromelain incorporated wine. This was done using the Fourier Transform Infrared Spectroscopy-Bruker/Vertex 80 instrument (Chandra et al 2021).…”

“…The particle size analysis of the normal white wine and the best wine sample i.e. Bromelain (200 mg/L) incorporated wine; was carried out using the Particle Size Analyzer-Zeta Sizer -(Malvern) (Chandra et al 2021).…”