Effect of X-Irradiation on Cellular Inclusions in Chicken Embryo Livers

Schjeide, O. A.; Ragan, N. Beth; McCANDLESS, Ruth G.; Bishop, F.

doi:10.2307/3570953

Cited by 8 publications

(5 citation statements)

References 6 publications

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“…Subsequent to completion of the present work, Gschwendt (1977a) reported that injection of oestradiol into the 15-day embryonated egg provoked an increase in the liver nuclear oestrogen receptor. It had earlier been shown that injection of high doses of oestrogen into the yolk sac gave an increased serum concentration of a light lipoprotein at early stages of development (Schjeide et al, 1960). For the purpose of this study large doses of hormone, which would be likely to give maximal responses at each stage, were necessary.…”

Section: Resultsmentioning

confidence: 97%

“…The present study gives the first unambiguous evidence that the embryonic-chick liver is capable of the oestrogen-induced synthesis of vitellogenin. The findings of earlier workers were limited by insensitive or non-specific methods (Schjeide et al, 1960;Carinci et al, 1976). Direct induction of vitellogenin by physiological amounts of oestradiol added to organ cultures has not been demonstrated for chick liver, but it has been shown for liver from the toad Xenopus laevis (Wangh & Knowland, 1975;Green & Tata, 1976;Tata, 1976).…”

Section: Discussionmentioning

confidence: 99%

“…The only previous reported investigations into the capacity of embryonic liver to synthesize egg-yolk phosphoprotein were carried out by using ultracentrifugal analysis of serum after oestrogen treatment (Schjeide et al, 1960) or phosphoprotein P analysis of secreted protein (Carinci et al, 1976). An assay based on radiolabelling of vitellogenin synthesized by embryonic livers in vitro would be preferable.…”

Section: Vitellogenin Synthesis In Chick Liver In Vitromentioning

confidence: 99%

“…In the first study on the development of the ability of the chick embryo to produce 'phosvitin and lipovitellin' in response to injected oestradiol, Schjeide et al (1960) found that competence was achieved only on day 19-20 of embryonic development. Carinci et al (1976), however, reported that cultured liver from the 14-day chick embryo produced an uncharacterized phosphoprotein in response to oestradiol.…”

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confidence: 99%

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Ontogeny of the vitellogenic response to oestradiol and of the soluble nuclear oestrogen receptor in embryonic-chick liver

Lazier¹

1978

Biochemical Journal

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A specific high-affinity oestradiol-binding protein was characterized in salt extracts of liver nuclei of the developing chick embryo. It is present in very small amounts at day 10 of development and is marginally stimulated by oestradiol injection into the yolk sac on day 8. Injection of oestradiol on day 10 evokes a substantial increase in the nuclear oestradiol-binding activity measured on day 12 of development. This oestradiol-binding protein has properties of sedimentation, hormone specificity and high-affinity binding very similar to those of the soluble nuclear receptor in hatched chicks. Livers from the 12-day embryos injected 48 h earlier with oestradiol do not synthesize vitellogenin, as judged by a specific immunochemical and electrophoretic assay for this oestrogen-induced protein. Traces of vitellogenin synthesis can be induced in 13-day-embryo liver, and a substantial response, equivalent to that in hatched chicks, is seen in liver from 15-day embryos injected on day 13. The development of the ability of oestradiol to increase the concentration of the soluble nuclear receptor appears to be one, but not the only, critical factor involved in the development of the ability of chick liver to synthesize vitellogenin.

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Section: Resultsmentioning

confidence: 97%

Section: Discussionmentioning

confidence: 99%

Section: Vitellogenin Synthesis In Chick Liver In Vitromentioning

confidence: 99%

mentioning

confidence: 99%

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Ontogeny of the vitellogenic response to oestradiol and of the soluble nuclear oestrogen receptor in embryonic-chick liver

Lazier¹

1978

Biochemical Journal

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“…The present results may only partially support these contentions. Although 6-day chick-embryo liver cells are rapidly dividing and apparently synthesize little protein for export (Duck-Chong, 1963;Kaminski & Durieux, 1954Nace & Schechtman, 1948;Pickering & Gladstone, 1925;Schjeide et al, 1960), a large fraction of the cellular ribosomes were found to be membrane-bound (Table 6). Further, although a high concentration of free ribosomes was observed in 6-day liver cells, a corollary of these findings is that an equally high concentration of membrane-bound ribosomes is also present ( Table 6).…”

Section: Discussionmentioning

confidence: 99%

Changes in free and membrane-bound ribosomes during the development of chick liver

O’Toole

Pollak

1974

Biochemical Journal

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A major difficulty in studying quantitative changes in free and membrane-bound ribosomes in a tissue under different physiological conditions is that membrane-bound ribosomes are not usually recovered quantitatively in a conventional microsomal fraction. This problem was resolved for developing chick liver by determining the conditions for the isolation of a microsomal fraction containing the highest practicable yield of rough vesicles, and then separating it into free-ribosome- and rough-vesicle-containing fractions. With the aid of a marker enzyme for the microsomal membranes and the RNA content of the recovered membrane-bound ribosomes, it was possible to correct for the recovery of rough vesicles and hence to determine the concentration of membrane-bound ribosomes in the homogenate. Despite the fact that morphological studies have suggested that most of the cellular ribosomes are not bound to membrane in chick liver cells at the earliest developmental age studied (6 days of egg incubation), 49% of the total ribosomes were found to be membrane-bound by using the new fractionation technique. This fraction increased (to 66%) during development. The discrepancy between the cell-fractionation and morphological approaches could not be attributed to artifacts of the separation method but rather to difficulties inherent in the morphological approach.

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Low Density Lipoproteins of Nonmammalian Vertebrates

Schjeide

1976

Low Density Lipoproteins

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Effect of X-Irradiation on Cellular Inclusions in Chicken Embryo Livers

Cited by 8 publications

References 6 publications

Ontogeny of the vitellogenic response to oestradiol and of the soluble nuclear oestrogen receptor in embryonic-chick liver

Ontogeny of the vitellogenic response to oestradiol and of the soluble nuclear oestrogen receptor in embryonic-chick liver

Changes in free and membrane-bound ribosomes during the development of chick liver

Low Density Lipoproteins of Nonmammalian Vertebrates

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