Effect of Whole Blood Clotting Time in Rats With Ionized Hypocalcemia Induced by Rapid Intravenous Citrate Infusion

Fukuda, T; Nakashima, Yoshifumi; Harada, M; Toyoshima, Shigeki; Koshitani, Osamu; Kawaguchi, Yoshiro; Nakayama, Mitsuo

doi:10.2131/jts.31.229

Cited by 31 publications

(20 citation statements)

References 17 publications

(18 reference statements)

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“…Ionized calcium levels required for hemodynamic stability were kept within the physiological range [19, 20] by infusion of 10% calcium gluconate at fixed rates.…”

Section: Discussionmentioning

confidence: 99%

Citrate shows protective effects on cardiovascular and renal function in ischemia-induced acute kidney injury

et al. 2017

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BackgroundIschemia and reperfusion (I/R) is one of the major causes of acute kidney injury (AKI). Citrate reduces hypoxia-induced mitochondrial energetic deficits in isolated proximal tubules. Moreover, citrate anticoagulation is now frequently used in renal replacement therapy. In the present study a rat model of I/R-induced AKI was utilized to examine renal protection by citrate in vivo.MethodsAKI was induced by bilateral renal clamping (40 min) followed by reperfusion (3 h). Citrate was infused at three different concentrations (0.3 mmol/kg/h; 0.6 mmol/kg/h and 1.0 mmol/kg/h) continuously for 60 min before and 45 min after ischemia. Plasma calcium concentrations were kept stable by infusion of calcium gluconate. The effect of citrate was evaluated by biomonitoring, blood and plasma parameters, histopathology and tissue ATP content.ResultsIn comparison to the normoxic control group bilateral renal ischemia led to an increase of creatinine and lactate dehydrogenase activity and a decrease in tissue ATP content and was accompanied by a drop in mean arterial blood pressure. Infusion of 1.0 mmol/kg/h citrate led to lower creatinine and reduced LDH activity compared to the I/R control group and a tendency for higher tissue ATP content. Pre-ischemic infusion of 1.0 mmol/kg/h citrate stabilized blood pressure during ischemia.ConclusionsCitrate has a protective effect during I/R-induced AKI, possibly by limiting the mitochondrial deficit as well as by beneficial cardiovascular effects. This strengthens the rationale of using citrate in continuous renal replacement therapy and encourages consideration of citrate infusion as a therapeutic treatment for AKI in humans.Electronic supplementary materialThe online version of this article (doi:10.1186/s12882-017-0546-1) contains supplementary material, which is available to authorized users.

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“…Ionized calcium levels required for hemodynamic stability were kept within the physiological range [19, 20] by infusion of 10% calcium gluconate at fixed rates.…”

Section: Discussionmentioning

confidence: 99%

Citrate shows protective effects on cardiovascular and renal function in ischemia-induced acute kidney injury

et al. 2017

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“…Association of Ca levels with changes in clotting time and bleeding tendency was shown in the rodent models. 8,9 Thus, serum Ca levels might play a pivotal role in hemostasis in acute ICH.…”

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confidence: 99%

Low Serum Calcium Levels Contribute to Larger Hematoma Volume in Acute Intracerebral Hemorrhage

Inoue

Miyashita

Toyoda

et al. 2013

Stroke

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“…One group of rats with administration of aspirin (Sigma-Aldrich) orally and another untreated group were included as controls. The blood clotting time and thrombus wet weight were measured as previously described (Fredrich et al, 1994;Fukuda et al, 2006). Serum samples were collected from each rat for further experiment.…”

Section: Animals and Samplesmentioning

confidence: 99%

Protective Role of Xinnaoning Tablet in Ischemic Stroke in Rat Model

Lv¹,

Xie²,

Feng³

et al. 2018

AJTCAM

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Background: Stroke has been considered as the second leading cause of death worldwide. The survivors of stroke experience different level of impair brain function. In China, Chinese traditional medicine had been widely accepted for stroke therapy and prevention. In this study, we developed Traditional Chinese Medicine based Xinnaoning (peace of heart and brain) Tablet and tested its protective role for ischemic stroke in rat model. Material and Methods: Male Wistar rats (n=60) with 12 weeks old and weight from 180 to 200 gram were randomly divided to five groups (n=12). For the groups with Xinaoning administration, the drug was administrated to rats once per day for 7 consecutive days. The blood clotting time and the thrombus wet weight was measured. Serum samples were collected from each rat for further Measurement of biochemical indicators. Results: Our results demonstrated that Xinnaoning tablet reduced lactate acid (LD) level and increased lactic acid dehydrogenase (LDH) in cerebral ischemia model as well as reduced the infarct size caused by stroke. Besides, evaluation of the level of different ATPases suggested Xinnaoning tablet could modulate ATPases activity and confer a protective role in brain. Moreover, analysis indicated Xinnaoning tablet have the anti-coagulation effect in vivo which may contribute to the protection of ischemia. Conclusion: Our findings suggest that Xinnaoning tablet may be a potential way for cerebral ischemia prevention.

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Effect of Whole Blood Clotting Time in Rats With Ionized Hypocalcemia Induced by Rapid Intravenous Citrate Infusion

Cited by 31 publications

References 17 publications

Citrate shows protective effects on cardiovascular and renal function in ischemia-induced acute kidney injury

Citrate shows protective effects on cardiovascular and renal function in ischemia-induced acute kidney injury

Low Serum Calcium Levels Contribute to Larger Hematoma Volume in Acute Intracerebral Hemorrhage

Protective Role of Xinnaoning Tablet in Ischemic Stroke in Rat Model

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