Effect of wheat bran on the health and performance of weaned pigs challenged with Escherichia coli K88+

Molist, F.; Segura, A. Gómez de; Pérez, J. F.; Bhandari, S. K.; Krause, Denis O.; Nyachoti, C. M.

doi:10.1016/j.livsci.2010.06.067

Cited by 50 publications

(37 citation statements)

References 11 publications

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“…The inclusion of wheat by-products, such as wheat bran, rich in insoluble NSP, resistant to fermentation, can reduce bowel transit time [8], which could explain some of the changes found. Actually, wheat bran has been reported to reduce pathogen adhesion [56,57] and to promote the Prevotellaceae family [11,58]. Another non-energetic carbohydrate source that could have promoted changes in particular microbial groups is chicory pulp.…”

Section: Discussionmentioning

confidence: 99%

Applicability of an Unmedicated Feeding Program Aimed to Reduce the Use of Antimicrobials in Nursery Piglets: Impact on Performance and Fecal Microbiota

López-Colom

Estellé

Bonet

et al. 2020

Animals

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This study aimed to assess the impact of two different feeding programs, including or not antimicrobials, on gut microbiota development at early ages in commercial pigs. For this, 21-day-old weaned piglets were distributed into 12 pens (6 replicates with 26 pigs each) and fed ad libitum until fattening with: standard commercial formula with antibiotics and zinc oxide (2400 ppm) (AB), and alternative unmedicated feed formula (UN). Subsequently, the animals were moved to the fattening unit (F) receiving a common diet. Pigs were weighed, and feed consumption and diarrhea scores registered. Feces were collected on days 9 (pre-starter), 40 (starter) and 72 (fattening) post-weaning and microbial DNA extracted for 16S rDNA sequencing. Piglets fed UN diets had a worse feed efficiency (p < 0.05) than AB during nursery; however, UN pigs spent less time scouring after weaning (p = 0.098). The structure of fecal community evolved with the age of the animals (p = 0.001), and diet also showed to have a role, particularly in the starter period when UN microbiomes clustered apart from AB, resembling the ecosystems found in the fattening animals. Fibrolytic genera (Fibrobacter, Butyrivibrio, Christellansellaceae) were enriched in UN piglets whereas Lactobacillus characterized AB piglets (adjusted p < 0.05). Overall, this alternative feeding program could anticipate the gut development of piglets despite a lower feed efficiency compared to standard medicalized programs.

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Section: Discussionmentioning

confidence: 99%

Applicability of an Unmedicated Feeding Program Aimed to Reduce the Use of Antimicrobials in Nursery Piglets: Impact on Performance and Fecal Microbiota

López-Colom

Estellé

Bonet

et al. 2020

Animals

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“…In this sense, the hard outer layer of wheat grain, the bran (WB), whose main function is to protect the grain against pathogen invasion, could be a natural source of QQ compounds or other antimicrobial substances. Many beneficial effects have been described for WB (Mohsin-Javed et al, 2012), also including anti-adhesive properties against enteropathogens (Molist et al, 2009). In this regard, it has been previously demonstrated that WB extract has the potential to modify the intestinal microbiota of animals (Molist et al, 2009) and also to interfere with the binding capacity of enteropathogens such as Escherichia coli K88 to intestinal epithelial cells and mucus (Hermes et al, 2011;Gonzalez-Ortiz et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

“…Many beneficial effects have been described for WB (Mohsin-Javed et al, 2012), also including anti-adhesive properties against enteropathogens (Molist et al, 2009). In this regard, it has been previously demonstrated that WB extract has the potential to modify the intestinal microbiota of animals (Molist et al, 2009) and also to interfere with the binding capacity of enteropathogens such as Escherichia coli K88 to intestinal epithelial cells and mucus (Hermes et al, 2011;Gonzalez-Ortiz et al, 2013). These reported effects could be explained, between other factors, by a possible interference of the WB extract in the E. coli QS systems.…”

Section: Introductionmentioning

confidence: 99%

New properties of wheat bran: anti‐biofilm activity and interference with bacteria quorum‐sensing systems

González-Ortiz

Ufford

Halkes

et al. 2014

Environmental Microbiology

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Some plant extracts, have been demonstrated to interfere with the microbial metabolism of several pathogenic bacteria. Within this antimicrobial properties it has been described the potential to inhibit or destroy biofilms or to interfere in quorum-sensing (QS) systems. However, to our knowledge, no study exploring this potential of wheat-bran (WB) has been published. The purpose of the present study is to evaluate the anti-biofilm activity of WB against a cow mastitis strain of Staphylococcus aureus and also its possible interference with bacterial QS systems. The potential of inhibition and destruction of the biofilm was studied by different in vitro assays. Also, we tested the ability of WB to interfere in bacterial QS by degrading acyl-homoserine lactones (AHL) as one of the most studied QS signal molecules for Gram-negative bacteria. The soluble extract of WB at 0.5% showed anti-biofilm activity, inhibiting biofilm formation and also destroying it. Similarly, the > 300 kDa fraction from WB had significant anti-biofilm activity in both in vitro assays. The WB also showed a potential to interfere with bacterial QS systems, as it was demonstrated to contain certain lactonase activity able to reduce AHL concentration in the medium. The present study reveals two additional beneficial properties of WB extract never explored before, which may be related to the presence of defence compounds in the plant extract able to interfere with microbial biofilms and also QS systems.

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“…Additional investigations showed a reduction of the E. coli population in the ileum digesta, and more interestingly, a reduction of the enterotoxigenic E. coli (ETEC) K88 attached to the ileum mucosa was observed when piglets received WB in their diet [10]. Even though the arabinoxylooligosaccharides of WB were initially considered to promote the anti-adhesive properties against enteropathogens [8], preliminary data suggested the involvement of a proteinaceous compound, which could specifically recognize ETEC K88 [11].…”

Section: Introductionmentioning

confidence: 99%

A Proteinaceous Fraction of Wheat Bran May Interfere in the Attachment of Enterotoxigenic E. Coli K88 (F4+) to Porcine Epithelial Cells

et al. 2014

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Wheat bran (WB) from Triticum aestivum has many beneficial effects on human health. To the best of our knowledge, very little has been published about its ability to prevent pathogenic bacterial adhesion in the intestine. Here, a WB extract was fractionated using different strategies, and the obtained fractions were tested in different in vitro methodologies to evaluate their interference in the attachment of enterotoxigenic Escherichia coli (ETEC) K88 to intestinal porcine epithelial cells (IPEC-J2) with the aim of identifying the putative anti-adhesive molecules. It was found that a proteinaceous compound in the >300-kDa fraction mediates the recognition of ETEC K88 to IPEC-J2. Further fractionation of the >300-kDa sample by size-exclusion chromatography showed several proteins below 90 kDa, suggesting that the target protein belongs to a high-molecular-weight (MW) multi-component protein complex. The identification of some relevant excised bands was performed by mass spectrometry (MS) and mostly revealed the presence of various protease inhibitors (PIs) of low MW: Serpin-Z2B, Class II chitinase, endogenous alpha-amylase/subtilisin inhibitor and alpha-amylase/trypsin inhibitor CM3. Furthermore, an incubation of the WB extract with ETEC K88 allowed for the identification of a 7S storage protein globulin of wheat, Globulin 3 of 66 kDa, which may be one of the most firmly attached WB proteins to ETEC K88 cells. Further studies should be performed to gain an understanding of the molecular recognition of the blocking process that takes place. All gathered information can eventually pave the way for the development of novel anti-adhesion therapeutic agents to prevent bacterial pathogenesis.

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Effect of wheat bran on the health and performance of weaned pigs challenged with Escherichia coli K88+

Cited by 50 publications

References 11 publications

Applicability of an Unmedicated Feeding Program Aimed to Reduce the Use of Antimicrobials in Nursery Piglets: Impact on Performance and Fecal Microbiota

Applicability of an Unmedicated Feeding Program Aimed to Reduce the Use of Antimicrobials in Nursery Piglets: Impact on Performance and Fecal Microbiota

New properties of wheat bran: anti‐biofilm activity and interference with bacteria quorum‐sensing systems

A Proteinaceous Fraction of Wheat Bran May Interfere in the Attachment of Enterotoxigenic E. Coli K88 (F4+) to Porcine Epithelial Cells

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