Effect of water stress on abscisic acid levels in white lupin (Lupinus albus L.) fruit, leaves and phloem exudate

Hoad, G. V.

doi:10.1007/bf00385079

Cited by 68 publications

(34 citation statements)

References 11 publications

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“…However, translocation obstruction does not alter leaf water potentials (26), so if such treatments change ABA biosynthesis or catabolism, an alternative regulatory mechanism would be necessary to explain the observed changes in ABA level in the present study. Evidence of substantial ABA translocation from leaves into sinks has been demonstrated in experiments involving [14CJABA application to leaf blades of Phaseolus (1 1) and wheat (3,8), and ABA has been found in phloem exudate (12). We suggest that in the present study, ABA might have accumulated in leaves because its normal phloem export to other plant parts was slowed or blocked.…”

Section: Setter Brun and Brennersupporting

confidence: 59%

Effect of Obstructed Translocation on Leaf Abscisic Acid, and Associated Stomatal Closure and Photosynthesis Decline

Setter¹,

Brun

Brenner³

1980

Plant Physiol.

111

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Pod removal or petiole girdling, which causes obstruction of translocation, was found in our previous study to cause reduced rates of photosynthesis in soybean leaves due to stomatal closure. The purpose of this research was to determine the involvement ofphotoassimilate accumulation and leaf abscisic acid (ABA) levels in the mechanism of stomatal closure induced by such treatments.Leaf glucose and sucrose levels increased during the initial 12-hour period after depodding or petiole girdling. Starch, which represents a much larger pool of leaf carbohydrate, was not perceptibly increased above control levels during the 12-hour posttreatment period. When leaflets were exposed to nonphotosynthetic enviromments (shading or COrfree air) for a 24-hour period after the translocation-obstructing treatments were applied and then returned to normal light or CO2 concentration, stomatal diffusive conductivity was reduced 65% and 85% with depodding and girdling, respectively. These reductions were comparable to those previously observed without an intervening nonphotosynthetic exposure, thus indicating that photosynthate accumulations were not necessary for the observed response.Free and bound ABA (released on alkaline hydrolysis) were determined by gas liquid chromatography with electron capture detection following preparative high performance liquid chromatography. Free ABA in monitored leaves increased almost 10-fold 48 hours after complete depodding and 25-fold 24 hours after petiole girdling of such leaves. By 3 hours after treatment, in a time course study, free ABA had increased 2-fold above control values in depodded and 5-fold in girdled leaves. Leafconcentrations of bound ABA did not appear to be related to the treatment effects on stomata.Thus, the translocation-obstructing treatments cause an increased level of ABA by a mechanism not involving accumulation of photoassimilate.Increased leaf ABA levels, which were independent of water stress or leaf water potential, appear to be involved in the stomatal closure response. It is suggested that the mechanism of increased leaf ABA levels following translocation-obstruction may be due to an interference with normal translocation of ABA out of leaves. in photosynthetic CO2 fixation rates, in part or entirely, by inducing stomatal closure (5,9,15,16,18,26). In soybeans we found, within 48 h after depodding or petiole girdling treatment, reductions of 70 to 90%o in CER3 due to partial stomatal closure (26). Such treatments can also cause reduced rates of CER by decreasing the gm to CO2 diffusion (nonstomatal factors). Some investigators have suggested that as a result of these decreases in gm, leaf intercellular space CO2 concentrations increase, thus causing stomatal closure (4, 9). This explanation cannot hold, however, in short-term (c 24 h) studies where no effect was found on CER apart from stomatal closure (16,26).Data from source/sink manipulation studies, where experimentally reduced translocation fluxes are associated with reduced rates of photosynthesis, ...

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Section: Setter Brun and Brennersupporting

confidence: 59%

Effect of Obstructed Translocation on Leaf Abscisic Acid, and Associated Stomatal Closure and Photosynthesis Decline

Setter¹,

Brun

Brenner³

1980

Plant Physiol.

111

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“…In detached leaves the rise in [ABA] in response to water stress results largely from an increased rate of synthesis (Pierce & Raschke, 1981;Zeevaart, 1980;Murphy, 1984), while during rehydration synthesis is decreased and the rate of metabolism increased (Pierce & Raschke, 1981) leading to a rapid fall in [ABA]. In intact plants there is evidence for significant export of ABA from mature leaves (Goldbach & Goldbach, 1977;Zeevaart, 1977;Setter, Brun & Brenner, 1981;Zeevaart & Boyer, 1984) via phloem-mediated translocation (Hoad, 1973(Hoad, ,1978Zeevart, 1977;Hoad & Gaskin, 1980;Setter, Brun & Brenner, 1980;Weiler & Ziegler, 1981;Everat-Bourbouloux, 1982;Zeevaart & Boyer, 1984). However, both Hartung (1976) and Hoad (1978) have suggested that the export of ABA may be reduced during water stress.…”

Section: Introductionmentioning

confidence: 99%

“…In intact plants there is evidence for significant export of ABA from mature leaves (Goldbach & Goldbach, 1977;Zeevaart, 1977;Setter, Brun & Brenner, 1981;Zeevaart & Boyer, 1984) via phloem-mediated translocation (Hoad, 1973(Hoad, ,1978Zeevart, 1977;Hoad & Gaskin, 1980;Setter, Brun & Brenner, 1980;Weiler & Ziegler, 1981;Everat-Bourbouloux, 1982;Zeevaart & Boyer, 1984). However, both Hartung (1976) and Hoad (1978) have suggested that the export of ABA may be reduced during water stress.…”

Section: Introductionmentioning

confidence: 99%

REGULATION OF ABSCISIC ACID CONCENTRATION IN LEAVES OF FIELD‐GROWN PEARL MILLET (PENNISETUM AMERICANUM [L.] LEEKE): THE ROLE OF ABSCISIC ACID EXPORT ^*

et al. 1986

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SUMMARYDiurnal changes in the concentration of abscisic acid (ABA) which occur in leaves of droughted, field-grown plants of pearl millet {Pennisetum americanum [L.] Leeke) are not always correlated with changes in bulk leaf water potential (*P). A rapid decline in ABA content of the leaves following its rise to a peak level in mid-morning, was observed in several time-course studies despite continued water stress.The possibility that the reduction in ABA in leaves was due to an elevated rate of its export was examined in two ways: (i) by measuring ABA concentrations in developing panicles (possible sinks for leaf-produced ABA) and in leaves, and (ii) by comparing the amounts of ABA in ungirdled leaves and in leaves heat-girdled at the base of the lamina to block export. ABA concentrations in panicles generally paralleled those in leaves, though the peak level of ABA in the morning in panicles occurred later than in the leaves in some samplings. Although girdling initially increased ABA concentration, it did not prevent a subsequent fall which generally paralleled the decline observed in untreated leaves. The decrease in ABA that occurred despite the block to export and despite continuing stress was, therefore, attributed to changes in the synthesis or metabolism of ABA within the leaf.The probable rate of export of ABA from leaves, calculated from the changes in its concentration due to girdling, was highest at the time of most rapid ABA accumulation and declined thereafter. The percentage export of recently assimilated carbon declined similarly. However, the probable absolute rate of export of pbotosyntbate, computed from stomatal conductance and ["CJ-export measurements, was not uniquely related to tbat of ABA.

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“…Substantial export of leaf-applied [14C]AbA has been observed in Phaseolus (7,10) and wheat (3,5) and AbA has been identified in phloem diffusates of white lupin (8) and Ricinus (24). If AbA is normally exported from leaves through the phloem, obstruction of such translocation might result in AbA accumulation in the leaves.…”

mentioning

confidence: 99%

Abscisic Acid Translocation and Metabolism in Soybeans following Depodding and Petiole Girdling Treatments

Setter

Brun²,

Brenner³

1981

Plant Physiol.

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It was found earlier that depodding and girdling treatments which obstruct translocation, result in increased leaf AbA levels and partial stomatal closure. In the present work (±) 12-'4Clabscisic acid (AbA) was introduced into leaves and the mass, and radioactivity of AbA and AbAmetabolites were analyzed folowing translocation obstruction to determine whether the increased AbA was due to higher rates of synthesis, or lower rates of catabolism or export. The (±) 12-14CIAbA was introduced into soybean (Meff.) leaves by injection into the petiole region. AbA and AbAmetabolites (phaseic acid IPAI, dihydrophaseic acid IDPAI, AbA-conjugate, and an unknown metabolite) were separated with preparative high performance liquid chromatography. Methyl esters of AbA (free and that released after hydrolysis of AbA-conjugate), PA and DPA were determined with gas chromatography using electron capture detection.

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Effect of water stress on abscisic acid levels in white lupin (Lupinus albus L.) fruit, leaves and phloem exudate

Cited by 68 publications

References 11 publications

Effect of Obstructed Translocation on Leaf Abscisic Acid, and Associated Stomatal Closure and Photosynthesis Decline

Effect of Obstructed Translocation on Leaf Abscisic Acid, and Associated Stomatal Closure and Photosynthesis Decline

REGULATION OF ABSCISIC ACID CONCENTRATION IN LEAVES OF FIELD‐GROWN PEARL MILLET (PENNISETUM AMERICANUM [L.] LEEKE): THE ROLE OF ABSCISIC ACID EXPORT ^*

Abscisic Acid Translocation and Metabolism in Soybeans following Depodding and Petiole Girdling Treatments

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Effect of water stress on abscisic acid levels in white lupin (Lupinus albus L.) fruit, leaves and phloem exudate

Cited by 68 publications

References 11 publications

Effect of Obstructed Translocation on Leaf Abscisic Acid, and Associated Stomatal Closure and Photosynthesis Decline

Effect of Obstructed Translocation on Leaf Abscisic Acid, and Associated Stomatal Closure and Photosynthesis Decline

REGULATION OF ABSCISIC ACID CONCENTRATION IN LEAVES OF FIELD‐GROWN PEARL MILLET (PENNISETUM AMERICANUM [L.] LEEKE): THE ROLE OF ABSCISIC ACID EXPORT *

Abscisic Acid Translocation and Metabolism in Soybeans following Depodding and Petiole Girdling Treatments

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REGULATION OF ABSCISIC ACID CONCENTRATION IN LEAVES OF FIELD‐GROWN PEARL MILLET (PENNISETUM AMERICANUM [L.] LEEKE): THE ROLE OF ABSCISIC ACID EXPORT ^*