Effect of Water Load, Saline Load and Lipton Tea on Urine Output and Urine Ph in Young Healthy Male and Female Human Subjects

Abstract

The study was aimed at comparing the diuretic effect of saline load, water load and Lipton tea in between healthy male and female undergraduate medical students (18-26yrs). They were randomly grouped into five groups having both male and female subjects : control(A),water(B), normal saline(C), Hypertonic saline,1%(D), Lipton tea(E). The day before the experiment, instructions were given; the experimental groups drank their solution 12ml/kg body weight then the urine output was measured at 30mins interval. Paired t-test was used to analyse result between male and female intra group difference in urine output and pH with significant level having p value ≤ 0.05. The data are presented as mean±SEM. In water group at 30mins showed diuretic effect in both male and female (132.6±31.44 and 223.8±76) respectively and peaked at 60mins (202.4±38.96 and 304±36.78) in male and female. Lipton tea also showed diuretic effect which started in the females at 30mins (68.2±8.92) while it started at 60mins(185.6±34.24) in the males. It is therefore concluded that male and female subjects have same response to volume loading, however they were little variations in their pattern of response to this volume loading.

Bioequivalence studies of cetirizine tablets using the urine excretion data of healthy Ghanaian male volunteers

“…The choice of male volunteers was because of the nature of the sample (urine samples) needed for the study. According to a study conducted by Omolaso et al, [ 27 ] studying the effect of Lipton tea, saline load and water load on both male and female urine, it was discovered that the male urine contained fewer biological impurities as compared to the female urine. Moreover, the convenience associated with obtaining urine samples from male volunteers as compared to female volunteers further informed the choice of all male selection.…”