Effect of water activity and temperature on growth ofAspergillus carbonarius andAspergillus tubingensis and their interactions on ochratoxin A production

Chiotta, María Laura; Sosa, Débora María; Ponsone, M.L.; Chulze, Sofía Noemí

doi:10.3920/wmj2013.1692

Cited by 12 publications

(6 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Changes in water and temperature stress may impact on Aspergillus section Nigri species and influence OTA contamination of grapes and grape-based products. Also, there may be changes in mycotoxin prevalence among the same species (Chiotta et al, 2014;Garcia-Cela et al, 2014;Gil-Serna et al, 2014).…”

mentioning

confidence: 99%

Climate change, food security and mycotoxins: Do we know enough?

Medina

Akbar

Baazeem

et al. 2017

Fungal Biology Reviews

201

151

View full text Add to dashboard Cite

Climate change (CC) scenarios are predicted to have significant effects on the security of staple commodities. A key component of this impact is the infection of such crops by mycotoxigenic moulds and contamination with mycotoxins. The impacts of CC on mycotoxigenic fungi requires examination of the impacts of the threeway interactions between elevated CO2 (350-400 vs 650-1200 ppm), temperature increases (+2-5 o C) and drought stress on growth/mycotoxin production by key spoilage fungi in cereals and nuts. This review examines the available evidence on the impacts of interacting CC factors on growth and mycotoxin production by key mycotoxigenic fungi including Alternaria, Aspergillus, Fusarium and Penicillium species. Aspergillus flavus responsible for producing aflatoxin B1 (AFB1) is a class 1A carcinogen and its growth appears to be unaffected by CC factors. However, there is a significant stimulation of AFB1 production both in vitro and in vivo in maize. In contrast, studies on Aspergillus section Circumdati and Nigri species responsible for ochratoxin A contamination of a range of commodities and F. verticillioides and fumonisins suggest that some species are more resilient than others, especially in terms of mycotoxin production. Acclimatisation of mycotoxigenic fungal pathogens to CC factors may result in increased disease and perhaps mycotoxin contamination of staple cereals. Predictive modelling approaches to help identify regions where maximum impact may occur in terms of infection by mycotoxigenic fungi and toxin contamination of staple crops is hindered by the lack of reliable inputs on effects of the interacting CC factors. The present available knowledge is discussed in the context of the resilience of staple food chains and the impact that interacting CC factors may have on the availability of food in the future.

show abstract

mentioning

confidence: 99%

Climate change, food security and mycotoxins: Do we know enough?

Medina

Akbar

Baazeem

et al. 2017

Fungal Biology Reviews

201

151

View full text Add to dashboard Cite

show abstract

“…The relatively high temperature might have induced the germination of spores. After the germination, fungi started to grow and produced OTA (27,28). Surprisingly, the control group was not contaminated until six months later than the dipping pretreated sultanas, and the content of OTA in the control sultanas was significantly lower (p≤0.05) under the same storage conditions.…”

Section: Resultsmentioning

confidence: 72%

The effect of dipping pretreatment on ochratoxin A accumulation in sultanas and currants

Zhang

Wang

et al. 2016

Food Sci Biotechnol

View full text Add to dashboard Cite

The dipping pretreatment on the occurrence of ochratoxin A (OTA) in sultanas and currants was investigated. Grape samples were divided into two groups before a drying process. One group was dipped with potassium carbonate-ethyl oleate, and the other group was left without treatment (the control). OTA were detected using solid-phase extraction clean-up and a high-performance liquid chromatography fluorescence detector. Results showed that OTA content was below the limit of detection in the fresh grapes of both varieties. OTA were not detected in dried vine fruits before storage. However, a real naturally environmental storage revealed that the dipping pretreated samples were contaminated with OTA earlier and to a greater extent than the control. The OTA concentration in the treated and control samples after one-year storage was 0.22 and 0.19 μg/kg for sultanas and 0.34 and 0.21 μg/kg for currants, respectively. These results indicated that dipping pretreatment might increase the toxin contamination and safety risk.

show abstract

“…Notably, vineyard A contained samples with higher proportions of A. carbonarius, the major OTA-producing Aspergillus species in California vineyards; and vineyard A also had higher incidence of OTA contamination than vineyard B, which showed comparatively low A. carbonarius populations and minimal OTA contamination. In in vitro studies of A. tubingensis and A. carbonarius in co-culture, production of OTA by A. carbonarius was not affected by the presence of A. tubingensis at 15 to 288C, but OTA production was reduced when the two species were in co-culture at 358C (7,13). Because vineyard temperatures in Fresno, CA, typically exceed 358C during much of the growing season, it would be interesting to test whether this phenomenon is observable in the field.…”

Section: Resultsmentioning

confidence: 92%

Population Dynamics of Aspergillus Section Nigri Species on Vineyard Samples of Grapes and Raisins

Palumbo

O’Keeffe

et al. 2016

Journal of Food Protection

View full text Add to dashboard Cite

Several species of Aspergillus section Nigri, including potential mycotoxin producers, are common residents of grape vineyards, but the relative population size of individual species throughout the growing season is difficult to determine using traditional isolation and identification methods. Using a quantitative droplet digital PCR (ddPCR) method in combination with dilution plating, total Aspergillus section Nigri populations and relative proportions of A. niger, A. welwitschiae, A. carbonarius, and A. tubingensis were measured from vineyard samples without the need for identifying individual fungal isolates. Grapes were sampled from two raisin vineyards (vineyards A and B) at berry set, veraison, harvest, and raisin stages in two consecutive years. Plate counts showed that the total population of Aspergillus section Nigri present on the fruit increased from berry set to raisin and became a larger component of the total recovered fungal population in both vineyards in both years. Results from ddPCR analysis showed that the relative proportion of A. carbonarius among the four species assayed increased later in the season (harvest and raisin) in comparison to earlier in the season (berry set and veraison). Total fungal and Aspergillus section Nigri plate counts were not significantly different between vineyards in either year. However, vineyard A generally showed higher proportions of A. carbonarius in harvest and raisin samples than vineyard B. This coincided with higher incidence and levels of ochratoxin A in vineyard A harvest and raisin fruit than in vineyard B fruit. This work demonstrates that this ddPCR method is a useful tool for culture-independent monitoring of populations of mycotoxigenic Aspergillus species during grape and raisin production.

show abstract

Effect of water activity and temperature on growth ofAspergillus carbonarius andAspergillus tubingensis and their interactions on ochratoxin A production

Cited by 12 publications

References 29 publications

Climate change, food security and mycotoxins: Do we know enough?

Climate change, food security and mycotoxins: Do we know enough?

The effect of dipping pretreatment on ochratoxin A accumulation in sultanas and currants

Population Dynamics of Aspergillus Section Nigri Species on Vineyard Samples of Grapes and Raisins

Contact Info

Product

Resources

About