Effect of voluntary exercise on H2O2 release by subsarcolemmal and intermyofibrillar mitochondria

Servais, Stéphane; Couturier, Karine; Koubi, Harry; Rouanet, Jean‐Louis; Desplanches, Dominique; Sornay-Mayet, Marie-Hélène; Semporé, B.; Lavoie, Julie L.; Favier, R.

doi:10.1016/s0891-5849(03)00177-1

Cited by 94 publications

(82 citation statements)

References 43 publications

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“…Durante o exercício físico ocorre aumento significativo na produção de ATP, o qual está associado a aumento do fluxo mitocondrial (3) . Nossos achados demonstram que o treinamento físico regular de oito semanas aumenta a atividade da SDH e dos quatro complexos da CTE.…”

Section: Discussionunclassified

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Comparação do treinamento físico de quatro e oito semanas sobre atividade da cadeia transportadora de elétrons e marcadores de estresse oxidativo em fígado de camundongos

Silva

Ronsani

Souza

et al. 2010

Rev Bras Med Esporte

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RESUMOO presente estudo investigou o efeito de quatro e oito semanas de treinamento físico sobre a atividade dos complexos da cadeia transportadora de elétrons (CTE) e os marcadores de estresse oxidativo em fígado de camundongos. Vinte e um camundongos (CF1, 30-35g) foram distribuídos nos seguintes grupos: não treinado (NT); treinado quatro semanas (T4); treinado oito semanas (T8). Quarenta e oito horas após a última sessão de treinamento os animais foram mortos por decapitação e o fígado foi retirado e estocado em -70ºC para posterior análise. Atividade da succinato desidrogenase (SDH), dos complexos I,II,III e IV da CTE, carbonilação de proteína, conteúdo total de tióis e a atividade da superóxido dismutase foram mensurados. Os resultados demonstram que apenas oito semanas de treinamento aumentam a atividade da SDH, dos quatro complexos da CTE, da superóxido dismutase, e o conteúdo total de tióis em relação ao grupo não treinado. Houve ainda diminuição na carbonilação de proteína no respectivo grupo em relação ao NT. Em conclusão, são necessárias oito semanas de treinamento para que ocorram aumento no funcionamento mitocondrial e melhora nos marcadores de estresse oxidativo em fígado de camundongos. Palavras-chave: treinamento físico, cadeia transportadora de elétrons, estresse oxidativo ABSTRACTThe present study investigated mitochondrial adaptations and oxidative stress markers after four and eight weeks of running training in liver of mice. Twenty-one male mice (CF1, 30-35g) were distributed into the following groups (n=7): untrained (UT); trained -four weeks (T4); trained -eight weeks (T8). Forty-eight hours after the last training session the animals were killed by decapitation and livers were removed and stored at -70ºC. Succinate dehydrogenase (SDH), complexes I, II, II-III and IV, protein carbonyls (PC), total thiol content and superoxide dismutase activity were measured. The results show that endurance training (8-wk) increases the SDH activity and complexes (I, II, III, IV), superoxide dismutase and total thiol content in liver when compared to untrained animals. Decrease in protein carbonylation in the respective group in relation to UT was also observed. It could be concluded that eight weeks of running training are necessary for mitochondrial respiratory chain enzyme activities increase and improvement in oxidative stress markers in liver of mice.Keywords: physical training, electron transport chain, oxidative stress INTRODUÇÃOEstudos têm reportado que o exercício físico aumenta a produção das espécies reativas de oxigênio (ERO) e, consequentemente, estresse oxidativo em diversos órgãos e tecidos (1,2) . Tem sido sugerido que a elevação do consumo de oxigênio induzida pelos exercícios aumenta a atividade mitocondrial, gerando altas concentrações de ERO (3) . O estresse oxidativo (EO) altera a integridade de constituintes celulares e compromete todo o funcionamento celular (1) . Células hepáticas precisam de energia para realizar diversas funções. A alta taxa metabólica do fígado (200kcal/kg/tecido...

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Section: Discussionunclassified

“…Tem sido sugerido que a elevação do consumo de oxigênio induzida pelos exercícios aumenta a atividade mitocondrial, gerando altas concentrações de ERO (3) . O estresse oxidativo (EO) altera a integridade de constituintes celulares e compromete todo o funcionamento celular (1) .…”

Section: Introductionunclassified

Comparação do treinamento físico de quatro e oito semanas sobre atividade da cadeia transportadora de elétrons e marcadores de estresse oxidativo em fígado de camundongos

Silva

Ronsani

Souza

et al. 2010

Rev Bras Med Esporte

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“…ROS are potent activators of mitochondrially mediated apoptosis, and their generation has been shown to be inversely related to the rates of mitochondrial respiration (2,24). As a result, we assessed ROS production and the rate of mitochondrial respiration in SS and IMF mitochondria during state 3 (active) and state 4 (passive) respiration.…”

Section: Discussionmentioning

confidence: 99%

Effect of prior chronic contractile activity on mitochondrial function and apoptotic protein expression in denervated muscle

O'Leary

Hood²

2008

Journal of Applied Physiology

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O'Leary MF, Hood DA. Effect of prior chronic contractile activity on mitochondrial function and apoptotic protein expression in denervated muscle. J Appl Physiol 105: 114 -120, 2008. First published May 1, 2008 doi:10.1152/japplphysiol.00724.2007.-Skeletal muscle is highly adaptable in response to increases and decreases in contractile activity. The purpose of this study was to determine whether the preconditioning of skeletal muscle has a protective effect against subsequent denervation-induced apoptotic protein expression. To investigate this, we chronically stimulated the tibialis anterior and extensor digitorum longus muscles for 7 days (10 Hz, 3 h/day) before 7 days of denervation. Denervation reduced total cytochrome-c oxidase activity by 39%, which was likely a consequence of a decrease in subsarcolemmal (SS) mitochondria. This decrease in the SS subfraction was prevented by prior chronic stimulation and, as a result, maintained total mitochondrial content at control levels. The expression of Bax was elevated 2.2-fold by denervation, and prior chronic stimulation did not attenuate this increase. This produced a increase in the Bax-to-Bcl-2 ratio, indicating greater muscle apoptotic susceptibility. Denervation also decreased state 3 respiration in SS and intermyofibrillar mitochondria and elevated state 4 reactive oxygen species production within both mitochondrial subfractions. These changes were not prevented by prior chronic stimulation. Furthermore, the antioxidant protein MnSOD was also reduced by denervation, whereas Beclin-1 was markedly elevated. This suggests that autophagic cell death could also play a significant part in denervationinduced muscle atrophy. Thus, despite prior chronic stimulation, denervation increases the apoptotic susceptibility of skeletal muscle by altering the Bax-to-Bcl-2 ratio, by increasing reactive oxygen species production, and by reducing the expression of MnSOD. Whether a more extensive stimulation paradigm would be more effective in attenuating apoptosis before muscle disuse remains to be determined. mitochondrial biogenesis; muscle atrophy; reactive oxygen species; autophagy; protein degradation SKELETAL MUSCLE IS AN ADAPTABLE tissue that exhibits a remarkable range of plasticity in response to different levels of contractile activity (15). In particular, chronic contractile activity is known to alter the expression of a variety of proteins from the nuclear and mitochondrial genomes (15). These changes in protein expression result in an increase in mitochondrial volume and density (6, 16). In skeletal muscle, two distinct subfractions of mitochondria exist that are characterized by their anatomic localization. Subsarcolemmal (SS) mitochondria are located just beneath the sarcolemmal membrane, whereas intermyofibrillar (IMF) mitochondria are intertwined between skeletal muscle myofibrils (8). SS and IMF mitochondrial subfractions are significantly increased in response to chronic contractile activity. However, SS mitochondria are more sensitive to endurance training a...

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“…Ochi et al (26) reported that β-titin, one of the strong potential candidates for change induced by eccentric contraction, was not affected by eccentric contraction. Studies showed that HSP27, αB-crystallin, HSP70, and HSP72 have different expression patterns following eccentric contraction (21,24,27,32). These results suggest that eccentric contraction probably induces individually different responses in each chaperone protein.…”

Section: Discussionmentioning

confidence: 99%

Muscle plasticity related to changes in tubulin and αB-crystallin levels induced by eccentric contraction in rat skeletal muscles

Eisuke

Sakurai

et al. 2016

Physiology International

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We used the model of eccentric contraction of the hindlimb muscle by Ochi et al. to examine the role of eccentric contraction in muscle plasticity. This model aims to focus on stimulated skeletal muscle responses by measuring tissue weights and tracing the quantities of αB-crystallin and tubulin. The medial gastrocnemius muscle (GCM) responded to electrically induced eccentric contraction (EIEC) with significant increases in tissue weight (p < 0.01) and the ratio of tissue weight to body weight (p < 0.05); however, there was a decrease in soleus muscle weight after EIEC. EIEC in the GCM caused contractile-induced sustenance of the traced proteins, but the soleus muscle exhibited a remarkable decrease in α-tubulin and a 19% decrease in αB-crystallin. EIEC caused fast-to-slow myosin heavy chain (MHC) isoform type-oriented shift within both the GCM and soleus muscle. These results have shown that different MHC isoform type-expressing slow and fast muscles commonly undergo fast-to-slow type MHC isoform transformation. This suggests that different levels of EIEC affected each of the slow and fast muscles to induce different quantitative changes in the expression of αB-crystallin and α-tubulin.

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Effect of voluntary exercise on H2O2 release by subsarcolemmal and intermyofibrillar mitochondria

Cited by 94 publications

References 43 publications

Comparação do treinamento físico de quatro e oito semanas sobre atividade da cadeia transportadora de elétrons e marcadores de estresse oxidativo em fígado de camundongos

Comparação do treinamento físico de quatro e oito semanas sobre atividade da cadeia transportadora de elétrons e marcadores de estresse oxidativo em fígado de camundongos

Effect of prior chronic contractile activity on mitochondrial function and apoptotic protein expression in denervated muscle

Muscle plasticity related to changes in tubulin and αB-crystallin levels induced by eccentric contraction in rat skeletal muscles

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