Effect of volatile citral on the development of blue mould, green mould and sour rot on navel orange

Wuryatmo, Erminawati; Able, Amanda J.; Ford, Christopher M.; Scott, Eileen S.

doi:10.1007/s13313-014-0281-z

Cited by 41 publications

(34 citation statements)

References 25 publications

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“…The antimicrobial effect of citral was also reported in fruit as shown in Table 1.1. Wuryatmo et al (2014) demonstrated that citral delayed the development of sour rot on oranges inoculated with Geotrichum citri-aurantii at room temperature and 5 C, supporting the effect previously observed in vitro. Siroli et al (2015a) and Belletti et al (2008) showed the antimicrobial properties of citral on fresh cut apples and fruit salad, reducing the viability of the spoilage microorganisms of the fruit such as lactic acid bacteria (LAB) and yeasts.…”

Section: Citralsupporting

confidence: 86%

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Antimicrobial packaging system for minimally processed fruit

Lledó¹,

Inés²

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Certifican que:La memoria titulada "Antimicrobial packaging system for minimally processed fruit" que presenta Dña. Marta Inés Lara Lledó para optar al grado de Doctor por la Universidad Politécnica de Valencia, ha sido realizada en el Instituto Tecnológico del Embalaje, Transporte y Logística (ITENE) bajo su dirección y que reúne las condiciones para ser defendida por su autora. antimicrobiano, a escala laboratorio y a escala semi-industrial, con el objetivo de reducir la proliferación de la flora natural de la fruta pelada y cortada y extender su vida útil. Se han desarrollado distintos prototipos para su posterior aplicación industrial.Previo al desarrollo de los materiales de envase, se llevó a cabo una selección de agentes activos más idóneos. Para ello se estudiaron mediante ensayos in vitro las propiedades antimicrobianas de agentes activos volátiles, citral, hexanal y linalool y diferentes mezclas de los mismos, frente a distintos microorganismos típicos del deterioro de las frutas, mohos y levaduras, concluyendo que la efectividad de la mezcla de los tres es superior a la suma de la efectividad de los agentes activos de forma individual. Así mismo, también se seleccionaron agentes antimicrobianos no volátiles como el sorbato potásico y benzoato sódico, los cuáles son ampliamente empleados en la industria alimentaria debido principalmente a sus propiedades antifúngicas.Con los agentes activos seleccionados, se desarrollaron películas monocapa de polipropileno (PP) con distintas concentraciones de la mezcla activa, citral, hexanal y linalool, a escala laboratorio, mediante técnicas de extrusión, y películas bicapa a escala semi-industrial mediante coextrusión. Por otra parte, se desarrollaron bandejas activas a escala semiindustrial mediante termoconformado de láminas obtenidas por coextrusión de compuestos de PP y etilvinilaceteto (EVA) con sorbato potásico o benzoato sódico como agentes antimicrobianos.Se evaluaron las propiedades mecánicas, barreras y térmicas de los materiales activos desarrollados, así como su sellabilidad y transparencia. En general, las propiedades de los polímeros no se vieron afectadas de manera relevante. Sin embargo, las bandejas activas perdieron su carácter transparente debido a la incorporación de los agentes activos no volátiles.Se estudió la cinética de liberación de los compuestos activos volátiles y no volátiles a distintas temperaturas, determinando los coeficientes de difusión de los agentes activos RESUMEN mediante el ajuste a modelos matemáticos de difusión basados en la Segunda Ley de Fick.Entre los agentes volátiles, el hexanal mostró un mayor coeficiente de difusión seguido de citral y linalool. Por otra parte, no hubo apenas diferencia en los coeficientes de difusión del sorbato potásico y benzoato sódico, siendo éstos del mismo orden de magnitud.Igualmente, se realizaron diferentes experimentos in vitro a distintas temperaturas para determinar las propiedades antimicrobianas de los materiales desarrollados. En general, los materiales activos presentaron una elev...

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Section: Citralsupporting

confidence: 86%

“…The antimicrobial activity of EOs and their components have been widely demonstrated in vitro against common spoilage and pathogenic microorganisms of fruit (Belletti et al, 2004;Combrinck et al, 2011;Wuryatmo et al, 2014;Wuryatmo et al, 2003;Zheng et al, 2015).…”

Section: Essential Oils and Their Pure Compoundsmentioning

confidence: 99%

Antimicrobial packaging system for minimally processed fruit

Lledó¹,

Inés²

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“…Plant essential oils or their volatile compositions have strong antifungal activity against Penicillium digitatum, P. italicum , and Galactomyces citriaurantii (Droby et al, 2008; Wuryatmo et al, 2003, 2014). The cell membrane, mitochondrion, and genetic material have also been considered major targets of volatile compound toxicity (Bakkali et al, 2008; Shao et al, 2013).…”

Section: Discussionmentioning

confidence: 99%

RNA Sequencing Reveals that Endoplasmic Reticulum Stress and Disruption of Membrane Integrity Underlie Dimethyl Trisulfide Toxicity against Fusarium oxysporum f. sp. cubense Tropical Race 4

et al. 2017

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Fusarium wilt of banana, a destructive disease that affects banana production, is caused by Fusarium oxysporum f. sp. cubense tropical race 4 (Foc TR4). In a previous study, we confirmed the strong inhibitory effects of Chinese leek (Allium tuberosum) on the incidence of this disease. Sulfur compounds are the primary antifungal constituents of Chinese leek. Among these, dimethyl trisulfide (DT) was the most abundant and exhibited the strongest inhibition of Foc TR4 growth and development. In the present study, the global gene expression profiles of Foc TR4 isolates treated with DT at 4,000-folds dilution (concentration of 1/4,000, v/v) for 1.5, 6, and 12 h were investigated by using RNA sequencing. The expression patterns of 15 DEGs were validated based on quantitative real-time PCR (qRT-PCR) assay. Untreated sample presented 2,556, 1,691, and 1,150 differentially expressed genes (DEGs) at 1.5, 6, and 12 h after the onset of the experiment, respectively, whereas DT-treated isolates presented 2,823, 3,546, and 6,197 DEGs. Based on Gene Ontology (GO) annotation and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis, DEGs involved in endoplasmic reticulum (ER), glycosylation, and steroid biosynthesis were significantly inhibited by DT exposure. The similar expressional patterns of 15 DEGs between RNA-seq and qRT-PCR assays indicated the reliability of the RNA-seq data. In conclusion, ER stress related to glycosylation inhibition and damage to cell membrane integrity might contribute to the toxicity of DT against Foc TR4. As the results presented here evidenced changes in gene expression associated with DT exposure, which might be used to develop new approaches for controlling FWB.

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“…Some essential oils (EOs) have shown antimicrobial effects against some postharvest pathogens using in vitro and in vivo tests (Bouchra, Mohamed, Mina, & Hmamouchi, ). In fact, thymol and carvacrol, either obtained from plant extracts or chemically synthesized, have been used to reduce decay caused by P. digitatum and P. italicum in lemon fruit (Castillo et al., ; Pérez‐Alfonso et al., ), and to control sour rot caused by G. citri‐aurantii in orange and lemon fruits (Boubaker et al., ; Regnier, Combrinck, Veldman, & Du Plooy, ; Wuryatmo, Able, Ford, & Scott, ; Xu et al., ). It has been observed that EOs from thyme and oregano applied to lemon fruit at concentrations above 1000 µL/L prevented arthroconidiale spore germination and mycelial growth of G. citri‐aurantii (Regnier et al., ).…”

Section: Introductionmentioning

confidence: 99%

Effect of Thymol and Carvacrol Encapsulated in Hp‐Β‐Cyclodextrin by Two Inclusion Methods against Geotrichum citri‐aurantii

Serna‐Escolano

Serrano

Valero

et al. 2019

Journal of Food Science

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Geotrichum citri‐aurantii causes sour rot in citrus fruits and is responsible for important economic losses during storage. However, the availability of chemical fungicides for the control of this pathogen is limited. Thus, the aim of this research was to evaluate the antifungal efficacy of thymol and carvacrol encapsulated in 2‐hydroxylpropyl‐beta‐cyclodextrin (HP‐β‐CD) (prepared by the microwave irradiation method [MW] and solubility method [S]) for inhibition of G. citri‐aurantii using in vitro bioassays broth (micro and macrodilutions methods) and inoculated food testing. Both encapsulated thymol and carvacrol were shown to be effective for inhibiting G. citri‐aurantii growth in in vitro assays. Thymol was more effective in inhibiting G. citri‐aurantii, while better encapsulation was provided by MW. HP‐β‐CD‐thymol encapsulated by MW (HP‐β‐CD‐thymol‐MW) showed the lowest 50% effective dose (ED50 = 1.16 mM), minimum inhibitory concentration (MIC = 5.06 mM), and minimum fungicide concentration (MFC = 52.6 mM). HP‐β‐CD‐thymol‐MW was found highly effective in reducing the growth rate and mycelial growth inhibition. Finally, HP‐β‐CD‐thymol‐MW and HP‐β‐CD‐carvacrol‐MW showed a higher persistent effect than thymol and carvacrol in their natural form in inhibiting this fungus. Therefore, HP‐β‐CD‐thymol‐MW could be a promising alternative to synthetic fungicides for controlling G. citri‐aurantii, the causal agent of citrus sour rot. Practical Application Encapsulated thymol and carvacrol in HP‐β‐Cyclodextrins are effective for controlling G. citri‐aurantii in in vitro experiments. Encapsulation of thymol and carvacrol by microwave irradiation method (MW) was more effective than the solubility (S) method. Thymol was more effective than carvacrol, and the best results on G. citri‐auriantii inhibition were achieved using the HP‐β‐CD‐thymol‐MW method (which gave the lowest ED50, MIC, and MFC).

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Effect of volatile citral on the development of blue mould, green mould and sour rot on navel orange

Cited by 41 publications

References 25 publications

Antimicrobial packaging system for minimally processed fruit

Antimicrobial packaging system for minimally processed fruit

RNA Sequencing Reveals that Endoplasmic Reticulum Stress and Disruption of Membrane Integrity Underlie Dimethyl Trisulfide Toxicity against Fusarium oxysporum f. sp. cubense Tropical Race 4

Effect of Thymol and Carvacrol Encapsulated in Hp‐Β‐Cyclodextrin by Two Inclusion Methods against Geotrichum citri‐aurantii

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