Effect of vitrification on human oocytes: a metabolic profiling study

Domı́nguez, Francisco; Castelló, D; Remohı́, J.; Simón, Carlos; Cobo, Ana

doi:10.1016/j.fertnstert.2012.09.034

Cited by 22 publications

(8 citation statements)

References 25 publications

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“…These data are comparable to those recently published regarding cycles with fresh and vitrified oocytes from our ovum donation program. Similar metabolomic profiles were also observed in embryos derived from fresh and vitrified oocytes, supporting the feasibility of accumulating oocytes or embryos for a single CCS cycle analysis [51]. …”

Section: Resultssupporting

confidence: 59%

New Tools for Embryo Selection: Comprehensive Chromosome Screening by Array Comparative Genomic Hybridization

Rodrigo

Mateu

Mercader

et al. 2014

BioMed Research International

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The objective of this study was to evaluate the usefulness of comprehensive chromosome screening (CCS) using array comparative genomic hybridization (aCGH). The study included 1420 CCS cycles for recurrent miscarriage (n = 203); repetitive implantation failure (n = 188); severe male factor (n = 116); previous trisomic pregnancy (n = 33); and advanced maternal age (n = 880). CCS was performed in cycles with fresh oocytes and embryos (n = 774); mixed cycles with fresh and vitrified oocytes (n = 320); mixed cycles with fresh and vitrified day-2 embryos (n = 235); and mixed cycles with fresh and vitrified day-3 embryos (n = 91). Day-3 embryo biopsy was performed and analyzed by aCGH followed by day-5 embryo transfer. Consistent implantation (range: 40.5–54.2%) and pregnancy rates per transfer (range: 46.0–62.9%) were obtained for all the indications and independently of the origin of the oocytes or embryos. However, a lower delivery rate per cycle was achieved in women aged over 40 years (18.1%) due to the higher percentage of aneuploid embryos (85.3%) and lower number of cycles with at least one euploid embryo available per transfer (40.3%). We concluded that aneuploidy is one of the major factors which affect embryo implantation.

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Section: Resultssupporting

confidence: 59%

New Tools for Embryo Selection: Comprehensive Chromosome Screening by Array Comparative Genomic Hybridization

Rodrigo

Mateu

Mercader

et al. 2014

BioMed Research International

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“…The information available on the neonatal outcome and the long-term follow-up of children conceived after oocyte cryopreservation is limited (Chian et al, 2008(Chian et al, , 2014Noyes et al, 2009;Wennerholm et al, 2009;Cobo et al, 2014) and only small studies have specifically addressed the safety of oocyte vitrification (Coticchio et al, 2009;Bonetti et al, 2011;Chen et al, 2012;Forman et al, 2012;Khalili et al, 2012;Monzo et al, 2012;Dominguez et al, 2013;Gugliemo et al, 2014;Konc et al, 2014;Nohales Có rcoles et al, 2014;Palmerini et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

Chromosomal meiotic segregation, embryonic developmental kinetics and DNA (hydroxy)methylation analysis consolidate the safety of human oocyte vitrification

Munck

Petrussa

Verheyen³

et al. 2015

MHR: Basic Science of Reproductive Medicine

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Oocyte vitrification has been introduced into clinical settings without extensive pre-clinical safety testing. In this study, we analysed major safetyaspects of human oocyte vitrification in a high security closed system: (i) chromosomal meiotic segregation, (ii) embryonic developmental kinetics and (iii) DNA (hydroxy)methylation status. Fresh and vitrified sibling oocytes from young donors after intracytoplasmic sperm injection (ICSI) were compared in three different assays. Firstly, the chromosomal constitution of the fertilized zygotes was deduced from array comparative genomic hybridization results obtained from both polar bodies biopsied at Day 1. Secondly, embryo development up to Day 3 was analysed by time-lapse imaging. Ten specific time points, six morphokinetic time intervals and the average cell number on Day 3 were recorded. Thirdly, global DNA methylation and hydroxymethylation patterns were analysed by immunostaining on Day 3 embryos. The nuclear fluorescence intensity was measured by Volocity imaging software. Comprehensive chromosomal screening of the polar bodies demonstrated that at least half of the zygotes obtained after ICSI of fresh and vitrified oocytes were euploid. Time-lapse analysis showed that there was no significant difference in cleavage timings, the predictive morphokinetic time intervals nor the average cell number between embryos developed from fresh and vitrified oocytes. Finally, global DNA (hydroxy)methylation patterns were not significantly different between Day 3 embryos obtained from fresh and from vitrified oocytes. Our data further consolidate the safety of the oocyte vitrification technique. Nevertheless, additional testing in young and older sub-fertile/infertile patients and sound follow-up studies of children born after oocyte cryopreservation remain mandatory.

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“…Last year, a metabolomic profiling study of egg donors (Dominguez et al, 2013) confirmed that oocyte vitrification does not disturb the metabolomic profile of day 3 embryos, based on a multivariate analysis of global metabolomics and amino acid profiles. Oocytes maintained good ultrastructural preservation before vitrification (e.g.…”

Section: Discussionmentioning

confidence: 97%

Oocyte versus embryo vitrification for delayed embryo transfer: an observational study

Herrero

Pareja

Aragonés

et al. 2014

Reproductive BioMedicine Online

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The aim of this observational prospective study was to compare multiple embryological and pregnancy outcomes for vitrified oocytes against the same outcomes using vitrified embryos in patients at risk for ovarian hyperstimulation syndrome. Ninety-six patients were included and allocated to vitrification of oocytes (Group 1) or embryos (Group 2). No statistical differences in baseline characteristics between groups were detected. Implantation rate was 30.6% versus 33.1%, and clinical pregnancy rate was 41.9% versus 7.1% in groups 1 and 2, respectively. A higher clinical spontaneous abortion rate occurred in group 2 (9.7% versus 21.9% for groups 1 and 2, respectively), but the same cumulative clinical pregnancy rate was observed after three embryo transfers (62.0% in group 1 and 69.6% in group 2). The ongoing pregnancy rate per patient was similar in both groups (56.0% and 54.3% in groups 1 and 2, respectively). Also, live birth rate per stimulation was similar (72.0% and 69.6% in groups 1 and 2, respectively). No differences were observed in outcomes according to vitrification timing. Oocyte vitrification achieved the same live birth rate as embryo vitrification.

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Effect of vitrification on human oocytes: a metabolic profiling study

Cited by 22 publications

References 25 publications

New Tools for Embryo Selection: Comprehensive Chromosome Screening by Array Comparative Genomic Hybridization

New Tools for Embryo Selection: Comprehensive Chromosome Screening by Array Comparative Genomic Hybridization

Chromosomal meiotic segregation, embryonic developmental kinetics and DNA (hydroxy)methylation analysis consolidate the safety of human oocyte vitrification

Oocyte versus embryo vitrification for delayed embryo transfer: an observational study

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