Effect of vitrification of in vitro matured prepubertal goat oocytes on embryo development after parthenogenic activation and intracytoplasmic sperm injection

Menéndez-Blanco, Irene; Soto-Heras, Sandra; Catalá, M. G.; Piras, Anna-Rita; Izquierdo, Dolors; Paramio, M. T.

doi:10.1016/j.cryobiol.2020.02.011

Cited by 12 publications

(9 citation statements)

References 29 publications

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“…At the end of IVF, the presumptive zygotes were fixed in ethanol absolute with Hoechst 33258 (10 μg/mL) at 4 °C [35], mounted on microscope slides covered with cover slips and observed under an inverted fluorescence microscope (Olympus IX70, Italy). Fertilization assessment was performed as follows [4]: -normal fertilization: male and female pronuclear formation (2PN) and extrusion of two polar bodies (PBs); -polyspermic fertilization: more than two pronuclei (>2PN) and two PBs; -asynchronous fertilization: one PN and a sperm head (SH) that failed chromatin decondensation and two PBs or one PN and MII plate and two PBs; -unfertilized oocytes: MII plate and first PB.…”

Section: Fertilization Assessmentmentioning

confidence: 99%

Protective effect of resveratrol against cadmium-induced toxicity on ovine oocyte in vitro maturation and fertilization

Piras

Ariu

Maltana

et al. 2022

J Animal Sci Biotechnol

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Background Heavy metal cadmium (Cd) is a widespread environmental contaminant with a potential toxicity that might negatively affect female reproduction and fertility. It has been reported that Cd exposure impaired the quality of oocytes and led to a defective maturation and fertilization, through oxidative stress induction. Resveratrol (Res) is a natural polyphenol with strong antioxidant properties that exhibited protective role in preventing oocyte redox homeostasis disruption and quality decline. Here, we explored whether the addition of Res to in vitro maturation (IVM) medium might act as a protection against Cd-induced toxicity on ovine oocyte maturation and fertilization. Firstly, we evaluated the effect of supplementing IVM medium with two different Res concentrations (1 and 2 μmol/L) on nuclear maturation and fertilization of oocytes matured under CdCl2 (2 μmol/L) exposure. Therefore, the concentration of 1 μmol/L Res was selected to analyse the effects of this compound on intracellular ROS levels, mitochondrial (mt) distribution and activity, chromatin configuration, cytoskeleton morphology, cortical granules (CGs) distribution and mRNA expression of genes associated with cellular response to oxidative stress (i.e. SIRT1, SOD 1, GPX1, GSR, CAT) in Cd-exposed in vitro matured oocytes. Results We found that 1 μmol/L Res restored the reduced oocyte meiotic competence induced by Cd exposure as well as, Res sustained oocyte ability to be normally fertilized and decreased polyspermic fertilization at both tested concentrations. Moreover, we demonstrated that 1 μmol/L Res mitigated Cd-induced alterations of oocyte cytoplasmic maturation by reducing reactive oxygen species (ROS) accumulation, preventing mt dysfunction, maintaining the correct meiotic spindle and cortical F-actin assembly and the normal cortical granule distribution as well as up-regulating SIRT1, SOD1 and GPX1 genes. Conclusions Taken together, our findings highlighted the beneficial influence exerted by Res in preventing Cd-induced disturbance of nuclear and cytoplasmic maturation and subsequent fertilization in ovine oocytes. Res treatment may help to establish defence strategies counteracting Cd-induced toxicity on the female gamete.

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Section: Fertilization Assessmentmentioning

confidence: 99%

Protective effect of resveratrol against cadmium-induced toxicity on ovine oocyte in vitro maturation and fertilization

Piras

Ariu

Maltana

et al. 2022

J Animal Sci Biotechnol

View full text Add to dashboard Cite

show abstract

“…At the end of IVF, the presumptive zygotes were fixed in ethanol absolute with Hoechst 33258 (10 μg/mL) at 4 °C [41], mounted on microscope slides covered with cover slips and observed under an inverted fluorescence microscope (Olympus IX70, Italy). Fertilization assessment was performed as follows [8]: -normal fertilization: male and female pronuclear formation (2PN) and extrusion of two polar bodies (PBs); -polyspermic fertilization: more than two pronuclei (>2PN) and two PBs;-asynchronous fertilization: one PN and a sperm head (SH) that failed chromatin decondensation and two PBs or one PN and MII plate and two PBs; -unfertilized oocytes : MII plate and first PB.…”

Section: Fertilization Assessmentmentioning

confidence: 99%

Protective Effect of Resveratrol Against Cadmium-Induced Toxicity On Ovine Oocyte In Vitro Maturation And Fertilization

Piras

Ariu

Maltana

et al. 2022

Preprint

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Background: Heavy metal cadmium (Cd) is a widespread environmental contaminant with a potential toxicity that might negatively affect female reproduction and fertility. It has been reported that Cd exposure impaired the quality of oocytes and led to a defective maturation and fertilization, through oxidative stress induction. Resveratrol (Res) is a natural polyphenol with strong antioxidant properties that exhibited protective role in preventing oocyte redox homeostasis disruption and quality decline. Here, we explored whether the addition of Res to in vitro maturation (IVM) medium might act as a protection against Cd-induced toxicity on ovine oocyte maturation and fertilization. Firstly, we evaluated the effect of supplementing IVM medium with two different Res concentrations (1 and 2 μmol/L) on nuclear maturation and fertilization of oocytes matured under CdCl2 (2 μmol/L) exposure. Therefore, the concentration of 1 μmol/L Res was selected to analyse intracellular ROS levels, mitochondrial (mt) distribution and activity, chromatin configuration, cytoskeleton morphology, cortical granules (CGs) distribution and mRNA expression of genes associated with cellular response to oxidative stress (i.e. SIRT1, SOD 1, GPX1, GSR, CAT ) in Cd-exposed in vitro matured oocytes. Results: We found that 1μmol/L Res was effective to recover the reduced oocyte meiotic competence induced by Cd exposure, while treatment with Res, at both tested concentrations, sustained oocyte ability to be normally fertilized and decreased polyspermic fertilization. Moreover, we demonstrated that 1 μmol/L Res mitigated Cd-induced alterations of oocyte cytoplasmic maturation by reducing reactive oxygen species (ROS) accumulation, preventing mt dysfunction, maintaining the correct meiotic spindle and cortical F-actin assembly and the normal cortical granule distribution as well as up-regulating SIRT1 , SOD1 and GPX1 genes. Conclusions: Taken together, our findings highlighted the beneficial influence exerted by Res in preventing Cd-induced disturbance of nuclear and cytoplasmic maturation and subsequent fertilization in ovine oocytes. Res treatment may help to establish defence strategies counteracting Cd-induced toxicity on the female gamete.

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“…The vitrification-warming procedure may play a vital role in the decreased developmental potential of oocytes. Meanwhile, it has been shown that vitrification may reduce oocyte competence by increasing dead oocytes and ROS levels [ 142 ]. When the ROS level exceeds the physiological limit, the oocyte developmental competence is compromised [ 81 , 143 ], while the rate of apoptosis is increased [ 144 , 145 ].…”

Section: Cryopreservation and Other Physical Factorsmentioning

confidence: 99%

Factors Influencing the In Vitro Maturation (IVM) of Human Oocyte

et al. 2021

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In vitro maturation (IVM) of oocytes is a promising assisted reproductive technology (ART) deemed as a simple and safe procedure. It is mainly used in patients with impaired oocyte maturation and in fertility preservation for women facing the risk of losing fertility. However, to date, it is still not widely used in clinical practice because of its underperformance. The influencing factors, such as biphasic IVM system, culture medium, and the supplementation, have a marked effect on the outcomes of oocyte IVM. However, the role of different culture media, supplements, and follicular priming regimens in oocyte IVM have yet to be fully clarified and deserve further investigation.

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Effect of vitrification of in vitro matured prepubertal goat oocytes on embryo development after parthenogenic activation and intracytoplasmic sperm injection

Cited by 12 publications

References 29 publications

Protective effect of resveratrol against cadmium-induced toxicity on ovine oocyte in vitro maturation and fertilization

Protective effect of resveratrol against cadmium-induced toxicity on ovine oocyte in vitro maturation and fertilization

Protective Effect of Resveratrol Against Cadmium-Induced Toxicity On Ovine Oocyte In Vitro Maturation And Fertilization

Factors Influencing the In Vitro Maturation (IVM) of Human Oocyte

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