Effect of Vitamins on the Quality of Insemination Doses of Bulls

Abstract

To preserve and protect spermatozoa against any possible oxidative damage the addition of natural antioxidants might be the ideal solution which has been investigated worldwide. The composition of the diluents is as follows: 50 µM/L vitamin C with 0.5% DMSO (dimethyl sulfoxide, Sigma, St. Louis, USA); 50 µM/L vitamin E with 0.5% ethanol; each added separately to the spermatozoa with the aim to determine its effect on post-thaw quality of spermatozoa. 20 samples from each control and experimental group were analysed. Semen was thawed at 37°C for 70 seconds. The sperm motility parameters were analysed immediately after thawing used Sperm Vision CASA (Computer Assisted Semen Analysis) system. The viability of the cells was evaluated by metabolic activity MTT assay and the nitroblue-tetrazolium (NBT) test was used to assess the intracellular formation of the superoxide radicals. Motility was observed with highest statistical differences (P0.05) with the samples containing vitamin C, however, higher percentage (54%) of motility was observed in comparison to the control group (51.61%). Based on MTT assay, viability was also improved with the supplementation of vitamin C with the highest significance (P0.05). however, had higher percentage of viable spermatozoa (105.8%) when compare to the control group (100.1%) which did not contain vitamin E. All of the mentioned substances used – vitamin C, vitamin E – prevented the intracellular overproduction of free radicals within the sperm mitochondrial membrane with the statistical significance (P

Protective effect of vitamin C and lycopene on the in vitro fertilization capacity of sex‐sorted bull sperm by inhibiting the oxidative stress

“…As shown in Figure 5a, our results showed that VC protected mitochondrial function by improving Δψm levels in sex-sorted bull sperm, confirming the results in non-sorted sperm in bovine (Hashim, Tvrdá, Greifová, & Lukáč, 2017) and human (Fanaei et al, 2014). It has been proven that VC can prevent intracellular overproduction of ROS (Hashim et al, 2017) and protect mitochondrial function (Fanaei et al, 2014) in sperm, which contributes to explaining the higher Δψm levels in our experiments.…”

Therapeutic Role of Green Tea Polyphenols in Improving Fertility: A Review

“…It also deactivates free radicals, protects the cellular membrane against O 2 free radicals, and prevents the production of ROS [ 54 ]. During cryopreservation, when vitamin E reacts with cryoprotectants, it can efficiently preserve the semen [ 55 , 56 , 57 ]. Vitamins E and C collectively enhance the intra-cytoplasmic semen injection (ICSI) success rate in patients with semen DNA damage and decrease the level of DNA impairment [ 58 ].…”