Effect of Vitamin E Acetate Supplementation on Thyroid Hormone-Sensitive Organs Following Exogenous L- Thyroxine Treatment

Abstract

Objective: L-thyroxine is used for control and prevention of many thyroidal diseases, though it may cause damages in thyroid hormone-sensitive organs, namely, liver and kidney. Reports on the protective effects of any antioxidants in L-thyroxine induced oxidative stress are scanty. Thus, L-thyroxine induced oxidative stress and its prevention by Vitamin E supplementation have been studied in the present investigation.Methods: Adult, male Wister rats were divided into four groups of six animals each, and L-thyroxine (T4) (0.3 mg/kg body weight) was administered intraperitoneally in the treated group. Similarly, L-thyroxine (T4), at the above-mentioned dose, and Vitamin E acetate (100 mg/kg of body weight/ day orally) coadministered simultaneously (T4+VE) in the next group. Third group was administered only with Vitamin E, and the remaining group kept as control. Treatment continued regularly for 15 and 30 days. Animals were sacrificed after completion of treatment. Lipid peroxidation (LPO) level, superoxide dismutase (SOD), catalase, and glutathione peroxidase (GPx) activities were assayed in liver and kidney along with their histology. Obtained results were interpreted statistically against their respective control groups.Results: Body weight was significantly decreased, and relative kidney weight was increased after L-thyroxine administration as compared to control (p<0.05). LPO level, SOD and catalase activities were significantly enhanced in L-thyroxine treated groups, whereas GPx activity was decreased. However, LPO level and the activities of those enzymes along with body weight and organ weights were almost restored their normal in L-thyroxine and Vitamin E coadministered group treated for 15 days and 30 days, respectively.Conclusion: Exogenously administered L-thyroxine causes oxidative stress in liver and kidney that in turn generates reactive oxygen species resulting cell damages. Vitamin E acetate supplementation reduces these adverse effects on liver and kidney and thus acts as a beneficial health management agent.

Morphometric parameters of thyroid gland, liver, and kidney on administration of thyroxine followed by Bauhinia Purpurea and Withania somnifera in female albino rats

“…The increased weight of the kidney on thyroxine administration stimulates the activity of adrenals indirectly and stimulates the adrenocorticosteroids on kidney function and growth of the kidney [14].The findings in our study agree with previous literature [13,14]. Majority of the studies suggest that thyroxine induces oxidative stress in different organs like kidney, liver led to increased weight of the organs, the same was noted in our study [4].The Bauhinia purpurea and withania somnifera at different doses against thyroxine administration showed remarkable changes in regulation of body weight of the animals, thyroid gland weight, liver ,and kidney weights may be due their anti-thyroid, antioxidant properties in management of thyroid health [4,15,16].…”

“…The hyperthyroidism occurs because of thyroid dysfunction due to over production of thyroid hormones [2]. The hormonal disorders affect the metabolic rate of the animals and lead to changes and disturbances in body weight of the animals directly and indirectly acts on thyroid sensitive organs [3].The long-term administration of thyroxine on thyroid and its sensitive organs produces free radicals due to oxidative stress [4]. Bauhinia purpurea leaf extract has anti-oxidant and anti-thyroid properties with its different flavonoids play a significant role against hyperthyroidism [5,6].The Combined effects of Bauhinia purpurea and ashwagandha extracts induced an increase in hepatic catalase activity, and suggested the hepatoprotective nature of the extracts in association with thyroid health [7].…”

Eltroxin and Hesperidin mitigate testicular and renal damage in hypothyroid rats: amelioration of oxidative stress through PPARγ and Nrf2/HO-1 signaling pathway

“…This synthetic drug elevates serum T3 and T4 levels to regulate the thyroid gland activity [ 8 ]. Prolonged use of a synthetic drug can treat HPO, but it may have adverse effects [ 9 ]. Therefore, researchers are interested in flavonoids because of their abundance in food, possible health benefits, and relatively low toxicity when compared to other phytochemicals [ 10 ].…”

Eltroxin Like Mimic Action of Withania Somnifera Leaf Extract in Hypothyroid-Induced Rats

“…Long duration usage of the synthetic drug can cure the hypothyroidism, but they cause side effects. L-thyroxin can damage the vital organs such as the liver and kidney, and it also causes the reduction in body weight and an increase in the relative body weight of the kidney [9]. Thus, there is a need to develop a drug from plants origin which can work for the hypothyroid treatment.…”