Effect of Vitamin D3 on the Calcium Binding Factor in Rat Intestinal Mucosa

Moriuchi, Sachiko; Ooizumi, Kumiko; Hosoya, Norimasa

doi:10.5925/jnsv1954.15.178

Cited by 8 publications

(5 citation statements)

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“…Much work has been done on the role of vitamin D3 in the stimulation of calcium absorp tion (1-3) and synthesis of calcium binding protein (4)(5)(6)(7) in the intestine, possibly being effected through RNA metabolism. In contrast, very few attempts have been made to explore the biological action of vitamin D on mito chondrial membrane in mammals.…”

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confidence: 99%

Effect of Vitamin D3 on Mitochondrial Membrane of Rat Liver

1971

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Effect of Vitamin D3 on Mitochondrial Membrane of Rat Liver

1971

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“…The vitamin in nuclei would act on the synthesis (12,27). On the other hand, membrane bound vitamin D3 would act on the membrane permeability for calcium, and this process did not involve protein syn thesis, and was not influenced by actinomycin D. Zull et al (10) and Norman (28) reported that actinomycin D treatment did not alter the tissue distribution of a did of 2000 and 350IU of 3H-vitamin D3 in vitamin D deficient rats and chicks, respectively.…”

Section: Discussionmentioning

confidence: 98%

“…In the previous paper, it was reported that calcium binding protein was mainly found in the distal part of small intestine, and this protein was in creased with vitamin D3 (12). It was known generally that calcium was absorbed by diffu sion in the distal, and it would be assumed that the calcium absorption in the distal would be a facilitated diffusion which required carrier protein.…”

Section: Discussionmentioning

confidence: 99%

Distribution of Orally Administered 45Ca in Rat Intestinal Tissues and Vitamin D3 Effect

Moriuchi¹,

Watanabe²,

Hosoya³

1970

J. Vitaminol

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The effects of vitamin D3 and actinomycin D on the radioactive calcium distribution were studied in the rat intestinal mucosa and muscle 5 and 30 minutes after the oral administration of 45Ca.1. Serum 45Ca concentration at 30min was increased significantly in the vitamin D3 treated rat, and this vitamin D3 effect was inhibited by actinomycin D. The action of vitamin D3 and actinomycin D was not observed at 5min. 2. 45Ca was observed in the proximal parts of intestinal mucosa (from pyloric end to 30cm) at 5min, and at 30min it came to be observed in the distal (40-60cm). 45Ca in the proximal parts at 5min was reduced with vitamin D3, and actinomycin D did not seem to be influenced on this vitamin D3 action. 45Ca in the distal (at 30min) was enhanced with vitamin D3 and this action was partially inhibited by actinomycin D. 3. 45Ca in muscle was lower than in mucosa. Observed pattern of 45Ca distribution at 5min in muscle was not so different in vitamin D deficient and D3 treated rats. Actinomycin D did not exert its action on muscle at 5min. Distribution patterns of 45Ca in muscle at 30min were almost similar to the mucosal patterns.4. Subcellular distribution of 45Ca was observed in the duodenal mucosa. Nuclei and cell debris (brush borders) have relative amounts of 45Ca 5min after 45Ca administration. At 30 minutes, 45Ca of subcellular fraction was significantly decreased, especially in nuclei, cell debris and mitochondria.This reduction was moderated by vitamin D3. Actinomycin D inhibited this vitamin D3 action in microsomes and supernatants.From these results, it was suggested that calcium transport would be carried out in two ways, membrane permeable system (actinomycin D insensitive) and the system involving calcium carrier protein (actinomycin D sensitive).

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“…In at least the chick (3,4), rat (6)(7)(8), dog (14), and monkey (16) CaBP is vitamin D-dependent, i.e., in the absence of vitamin D, synthesis of CaBP is reduced or absent.…”

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confidence: 99%

“…Intestinal calcium-binding protein (CaBP) has been detected in a wide variety of animals including frog, carp, and pike (1,2), chick (1,(3)(4)(5), rat (5)(6)(7)(8)(9), hamster ( l o ) , guinea pig2 (13), dog (14), pig (1, 5 , 13), bovine (15), horse (13), squirrel and cebus monkeys (16), and human (17)(18)(19)(20). In at least the chick (3,4), rat (6)(7)(8), dog (14), and monkey (16) CaBP is vitamin D-dependent, i.e., in the absence of vitamin D, synthesis of CaBP is reduced or absent.…”

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confidence: 99%