Effect of vitamin C and E supplementation on susceptibility of plasma lipoproteins to peroxidation induced by acute smoking

Harats, Dror; Ben-Naim, M.; Dabach, Y.; Hollander, G.; Havivi, E; Stein, O.; Stein, Y.

doi:10.1016/0021-9150(90)90181-h

Cited by 187 publications

(85 citation statements)

References 30 publications

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“…This way, vitamin C may function as an antioxidant even when the oxidation proceeds in the lipid domain, including the arterial wall. Vitamin C supplementation did not increase plasma a-tocopherol concentration, which is in agreement with previous supplementation studies in smokers (Harats et al, 1990;Fuller et al, 1996;Diaz et al, 1997;Neuzil et al, 1997). However, even then, the prooxidant/antioxidant balance in arterial tissue may be favourably influenced and protect LDL against oxidation (Mezzitti et al, 1995).…”

Section: Discussionsupporting

confidence: 91%

“…Further, the bioavailability was found to be incomplete for doses over 200 mg of vitamin C (Levine et al, 1996). The previous studies in healthy smokers on LDL susceptibility for oxidation applied daily doses of 500 mg (Harats et al, 1998), 1000 mg (Harats et al, 1990;Fuller et al, 1996) and 1500 mg (Harats et al, 1990) vitamin C, which resulted in 2.0-2.3-fold (Harats et al, 1990), 3.8-fold (Harats et al, 1998) to 3.9-fold (Fuller et al, 1996) increase in plasma ascorbate levels. In our study, the increase in mean plasma ascorbate was similar to the study of Harats et al (1990).…”

Section: Discussionmentioning

confidence: 94%

“…A number of 4-week supplementation studies in healthy smokers have indicated that vitamin C effectively reduced the in vitro susceptibility of LDL for oxidation (Fuller et al, 1996;Harats et al, 1998) and the concentration of plasma lipid peroxidation products such as conjugated dienes and TBARS (Harats et al, 1990(Harats et al, , 1998Fuller et al, 1996). A possible mechanism explaining why vitamin C might reduce the oxidative modification of LDL is by scavenging aqueous free radicals before they can attack and oxidize LDL.…”

Section: Discussionmentioning

confidence: 99%

“…The previous studies in healthy smokers on LDL susceptibility for oxidation applied daily doses of 500 mg (Harats et al, 1998), 1000 mg (Harats et al, 1990;Fuller et al, 1996) and 1500 mg (Harats et al, 1990) vitamin C, which resulted in 2.0-2.3-fold (Harats et al, 1990), 3.8-fold (Harats et al, 1998) to 3.9-fold (Fuller et al, 1996) increase in plasma ascorbate levels. In our study, the increase in mean plasma ascorbate was similar to the study of Harats et al (1990). The stronger increase in the other studies (Fuller et al, 1996;Harats et al, 1998) may partly be due to a low vitamin C diet and related low plasma ascorbate concentrations.…”

Section: Discussionmentioning

confidence: 99%

“…Vitamin C supplementation (500-2000 mg per day) has been shown to reduce the in vitro LDL oxidation susceptibility and plasma lipid peroxidation products thiobarbituric acid-reactive substrates (TBARS) (Harats et al, 1990(Harats et al, , 1998Fuller et al, 1996). In addition, trials in which an infusion of 10-18 mg vitamin C/min (or total dose about 1000 mg) was given to chronic smokers provided promising results for improved endothelial-dependent vasodilation (Heitzer et al, 1996;Motoyama et al, 1997).…”

Section: Introductionmentioning

confidence: 99%

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Does vitamin C supplementation influence the levels of circulating oxidized LDL, sICAM-1, sVCAM-1 and vWF-antigen in healthy male smokers?

et al. 2004

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Objective: To examine the effects of vitamin C supplementation on the concentration of oxidation markers, in particular, circulating oxidized LDL (OxLDL) and on endothelial activation markers. Design: Randomized double-blind, placebo-controlled crossover trial. Setting: Belgian population of the city of Leuven. Subjects: A total of 34 healthy male smokers aged 26-73 y. Intervention: Smokers were randomly assigned to receive either vitamin C (250 mg twice daily) or placebo capsules, each to be taken for 4 weeks. After a 1-week washout period, participants then crossed over to the alternative capsules for further 4 weeks. Mean outcome measures: Markers of oxidation (bilirubin, uric acid, a-tocopherol, retinol, malondialdehyde, circulating Oxidized LDL (OxLDL)) and markers of endothelial activation (sICAM-1, sVCAM-1, vWF-antigen) were analysed. Results: Plasma ascorbate concentrations significantly increased from 46.6717.6 to 70.1721.2 mmol/l after a 4-week treatment with 500 mg vitamin C per day. The other plasma antioxidants concentrations, including bilirubin, uric acid, a-tocopherol and retinol, were similar in both treatment periods. Vitamin C did not change plasma malondialdehyde and circulating OxLDL compared with placebo (vitamin C 0.7370.25 mg/dl OxLDL; placebo 0.7270.21 mg/dl OxLDL). After vitamin C supplementation, neither sICAM-1 and sVCAM-1 levels nor the concentration of vWF-antigen significantly differed from placebo condition. Conclusions: Oral supplementation of vitamin C is not associated with changes in markers of oxidation or endothelial activation in healthy male smokers.

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Section: Discussionsupporting

confidence: 91%

Section: Discussionmentioning

confidence: 94%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Does vitamin C supplementation influence the levels of circulating oxidized LDL, sICAM-1, sVCAM-1 and vWF-antigen in healthy male smokers?

et al. 2004

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Lipid peroxidationin vivo is induced by exercise on a bicycle ergometer in athletes

Wilson

Mansour

Stewart

et al. 2001

Eur. J. Lipid Sci. Technol.

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Lipid peroxidation in vivo is induced by exercise on a bicycle ergometer in athletesThere is a widely held belief that exercise leads to lipid peroxidation. Plasma hydroxy fatty acids originate from the diet and possibly from lipid peroxidation in vivo. We examined whether acute exercise (1 h at ~76% of predicted maximal aerobic capacity) leads to an increase in plasma hydroxy fatty acids without the confounding influence of the diet in 7 male athletes. In addition, using a random-sequence cross-over design, we examined whether vitamin C supplementation of 1 g per day for 10 days prior to exercise could prevent exercise-induced lipid peroxidation.Supplementation doubled the plasma vitamin C from 56.9 ± 3.4 to 112.7 ± 14.6 µM (P < 0.001) and lowered pre-exercise plasma hydroxy fatty acids from 1.30 ± 0.18 to 1.03 ± 0.14 µM (P < 0.05). Plasma hydroxy fatty acid levels rose up to 3-fold during exercise in some athletes on placebo and peak levels were reached after 20 min. The rise in plasma hydroxy fatty acid levels was delayed and remained lower after supplementation with vitamin C compared to placebo: 0.01 ± 0.07 vs. 0.63 ± 0.16 µM (P < 0.05). Vitamin C supplementation reduced pre-exercise creatine kinase levels (193 ± 52 vs. 277 ± 68 U/L; P < 0.05) but its effect on exercise-induced changes in creatine kinase did not reach statistical significance. In conclusion, large supplements of vitamin C reduced exercise-induced lipid peroxidation in athletes. This small, but carefully controlled study illustrates the usefulness of plasma hydroxy fatty acid levels to monitor lipid peroxidation in vivo in acute studies.

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Oxidized LDL and antioxidants

Jialal

Fuller

1993

Clin Cardiol

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Summary:Several studies have shown that the oxidative modification of low-density lipoprotein (LDL) may promote fattystreak formation, the early lesion of atherosclerosis. Epidemiologic data suggest that decreased levels of micronutrient antioxidants are associated with an increased frequency of cardiovascular disease. Micronutrients that can act as antioxidants-amrbic acid, a-tocopherol, and pCmtene-have been shown to prevent LDL oxidation in vitro and retard the progression of atherosclerosis in animal models. In addition, sup plementation of human subjects with these compounds have been shown to increase the resistance of their LDL to oxidation without producing side effects. Thus, these antioxidant micronutrients have the potential to become an additional treatment modality in the therapeutic arsenal against atherosclerosis.

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Effect of vitamin C and E supplementation on susceptibility of plasma lipoproteins to peroxidation induced by acute smoking

Cited by 187 publications

References 30 publications

Does vitamin C supplementation influence the levels of circulating oxidized LDL, sICAM-1, sVCAM-1 and vWF-antigen in healthy male smokers?

Does vitamin C supplementation influence the levels of circulating oxidized LDL, sICAM-1, sVCAM-1 and vWF-antigen in healthy male smokers?

Lipid peroxidationin vivo is induced by exercise on a bicycle ergometer in athletes

Oxidized LDL and antioxidants

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