Effect of Vit E on Secretion of HSP-70 in Testes of Broilers Exposed to Heat Stress

Abstract

The objective of this experiment was to investigate the ameliorative effect of vitamin E (Vit E) on tissue damage caused by heat stress in the testes of broilers, and to correlate this effect with the secretion of the HSP-70 protein. In the study totally 30 broilers (breed Ross 308) were used. Animals were divided into three groups, including the control group. Group Control (C); these broilers (24 o C) were not administered with Vit E in the diet and not subjected to heat stress. Group Stress (S); these broilers not administered with Vit E in the diet but subjected to heat stress (34 o C). Group Stress +Vitamin E (Group S + vit E); broilers subjected to heat stress (34 o C) and administered with vitamin E. Histopathological examination demonstrated the presence of degenerative alterations in testes tissue of the group S. However, there has been seen diminishing the severity of degeneration in the group S+Vit E. While secretion of HSP-70 was determined in the testes of all groups in varying degree, the highest secretion level of HSP-70 was in the group C, the lowest secretion level of HSP-70 was in the group S. It is concluded that heat stress reduces the secretion of HSP-70 in testes of broilers, when vitamin E is administered, the secretion of HSP-70 increases again and reduces tissues damage. Keywords: Heat stress, Broiler, Testis, Vit E, HSP-70 Sıcaklık Stresine Maruz Bırakılan Broilerlerin Testislerinde Vit E' in HSP-70 Sekresyonu Üzerine Etkisi ÖzetBu çalışmanın amacı broilerlerin testislerinde ısı stresinin sebep olduğu doku hasarı üzerine vitamin E (Vit E)'in iyileştirici etkisini araştırmak ve bu etkinin HSP-70 proteininin sekresyonu ile ilişkisini ortaya koymaktır. Çalışmada toplam olarak 30 adet (Ross 308 ırkı) broiler kullanıldı. Hayvanlar kontrol grubu da dahil olmak üzere üç gruba ayrıldı. Grup kontrol (C); sıcaklık stresine maruz bırakılmayan (24 o C) ve diyetlerine Vit E eklenmeyen broiler. Grup stress (S); diyetlerine Vit E eklenmeyen ancak sıcaklık stresine maruz bırakılan (34 o C) broiler. Grup Stres +Vitamin E (Grup S + vit E); sıcaklık stresine maruz bırakılan (34 o C) ve diyetlerine vitamin E eklenen broiler. Histopatolojik inceleme S grubunun testislerinde dejeneratif değişiklikler gösterdi. Ancak S + Vit E grubunda dejenerasyonun şiddetinin azaldığı gözlendi. Grupların tamamının testislerinde farklı seviyede HSP-70 sekresyonu saptanırken, HSP-70'in en yüksek seviyesi grup C'de en düşük seviyesi grup S'deydi. Isı stresinin broilerlerin testislerinde HSP-70 sekresyonunu azaltığı, vitamin E uygulandığında, HSP-70 sekresyonunun yeniden artığı ve doku hasarını azalttığı sonucuna varıldı.

Effect of cinnamon ( Cinnamomum zeylanicum ) bark oil on heat stress-induced changes in sperm production, testicular lipid peroxidation, testicular apoptosis, and androgenic receptor density in developing Japanese quails

“…Peritubular myoid cells, surrounding the seminiferous tubules [41], are smooth muscle cells [42]. It has been reported that cyclic guanosine monophosphatemediated phosphorylation of heat shock proteins [43], of which levels may increase or decrease under HS-induced oxidative stress [29,44], may cause smooth muscle relaxation [43]. One possible reason for the increased DST observed in this study after HS may be the relaxation of peritubular myoid cells.…”

“…Therefore, the findings obtained in this study are the first results regarding the effect of HS on testicular AR density in quails. Heat stress has been reported to cause the loss of spermatogenic cells and degenerative alterations in testis of broilers [32], the vacuolization of germinal epithelium, multinucleated giant cell formations in mice [33], the degeneration in seminiferous tubules, and the spermatogenic arrest in developing lambs [34] and adult llamas [35]. In this study, HS caused severe histopathologic lesions in the testes of quails such as increase in DST, decrease in GCLT along with disorganization and degeneration in germinal cells, spermatogenic arrest, poured immature spermatids, and spermatogonia in the lumen of some seminiferous tubules.…”

Effects of thermal manipulations during embryogenesis of broiler chickens on developmental stability, hatchability and chick quality

“…However, this development has led to an increase in sensitivity of the broilers to environmental factors, and has led to the weakening of their adaptability to adverse conditions (Uni and Yahav, 2010). The main environmental factors that cause loss of productivity and high mortality rates in poultry is heat stress, which is caused by high environmental temperature and humidity (Terim Kapakin et al, 2013). The feed consumption decreases in birds that are exposed to heat stress, and yield losses are experienced accordingly (Yalçin et al, 1997).…”

Dietary rosemary oil alleviates heat stress-induced structural and functional damage through lipid peroxidation in the testes of growing Japanese quail

“…Loss of spermatogenic cells, germ cell degeneration in seminiferous tubules, cessation of spermatogenesis in broilers (Terim Kapakın et al, 2013) and quail (Türk et al, 2015) as well as an increase in the DST, decrease in the GCLT along with germ cell disorganization in quail (Türk et al, 2015) have been reported to be HS-related outcomes along with an increase in some testicular histopathologic lesions. Similar histopathologic lesions including the increase in the DST, reduction in the GCLT, disorganization, and degeneration in germinal cells, cessation of spermatogenesis, existence of immature spermatids and spermatogonia in the lumen of some seminiferous tubules were detected in the testes of quail where HS was imposed on the birds in the present study.…”

“…Temperatures outside of the animal's thermo-neutral (TN) zone are often not optimal for testicular function and can potentially disrupt spermatogenesis (Durairajanayagam et al, 2014). Reductions have been reported for testis weight (McDaniel et al, 2004), semen volume, numbers of normal-shaped spermatozoa (Joshi et al, 1980), spermatozoa count, spermatozoa motility, and there is an increase in number of dead spermatozoa (McDaniel et al, 2004;Ebeid, 2012) as well as testicular degenerative disorders (Terim Kapakın et al, 2013;Türk et al, 2015) in different poultry species in response to HS.…”