Effect of vibration on osteoblastic and osteoclastic activities: Analysis of bone metabolism using goldfish scale as a model for bone

Suzuki, Nobuo; Kitamura, Kei-ichiro; Nemoto, Tetsu; Shimizu, Nobuaki; Wada, Shigehito; Kondo, Takashi; Tabata, M.; Sodeyama, F.; Ijiri, Kenichi; Hattori, Atsuhiko

doi:10.1016/j.asr.2007.04.104

Cited by 45 publications

(45 citation statements)

References 47 publications

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“…In osteoblasts, also, both InHg and MeHg inhibited the ALP enzyme activity at 18 and 36 hrs after incubation. It is known that the scales are calcified tissue that contains osteoclasts and osteoblasts (Bereiter-Hahn and Zylberberg, 1993;Yoshikubo et al, 2005;Suzuki et al, 2007;Suzuki et al, 2008;de Vrieze et al, 2010;Suzuki et al, 2011a;Thamamongood et al, 2012, Yano et al, 2013. Additionally, it has been reported that scales are a better potential internal calcium reservoir than body skeletons, jaws, and otoliths examined by the 45 Calabeling study of calcified tissues of goldfish and killifish (Mugiya and Watabe, 1977).…”

Section: Discussionmentioning

confidence: 99%

Effects of Inorganic Mercury and Methylmercury on Osteoclasts and Osteoblasts in the Scales of the Marine Teleost as a Model System of Bone

et al. 2014

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To evaluate the effects of inorganic mercury (InHg) and methylmercury (MeHg) on bone metabolism in a marine teleost, the activity of tartrate-resistant acid phosphatase (TRAP) and alkaline phosphatase (ALP) as indicators of such activity in osteoclasts and osteoblasts, respectively, were examined in scales of nibbler fish (Girella punctata). We found several lines of scales with nearly the same TRAP and ALP activity levels. Using these scales, we evaluated the influence of InHg and MeHg. TRAP activity in the scales treated with InHg (10 -5 and 10 -4 M) and MeHg (10 -6 to 10 -4 M) during 6 hrs of incubation decreased significantly. In contrast, ALP activity decreased after exposure to InHg (10 -5 and 10 -4 M) and MeHg (10 -6 to 10 -4 M) for 18 and 36 hrs, although its activity did not change after 6 hrs of incubation. As in enzyme activity 6 hrs after incubation, mRNA expression of TRAP (osteoclastic marker) decreased significantly with InHg and MeHg treatment, while that of collagen (osteoblastic marker) did not change significantly. At 6 hrs after incubation, the mRNA expression of metallothionein, which is a metal-binding protein in osteoblasts, was significantly increased following treatment with InHg or MeHg, suggesting that it may be involved in the protection of osteoblasts against mercury exposure up to 6 hrs after incubation. To our knowledge, this is the first report of the effects of mercury on osteoclasts and osteoblasts using marine teleost scale as a model system of bone.

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Section: Discussionmentioning

confidence: 99%

Effects of Inorganic Mercury and Methylmercury on Osteoclasts and Osteoblasts in the Scales of the Marine Teleost as a Model System of Bone

et al. 2014

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“…Our previous studies (Suzuki et al, 2007;Suzuki et al, 2008) using teleost scale as a bone model showed that osteoclastic activity significantly decreased to less than 2G under dynamic (vibration-driven) and static (centrifugally driven) acceleration loading. These results suggest that an acceleration of even 2G or less in the lumbar region caused by walking shows probability of suppressing bone absorption.…”

Section: Discussionmentioning

confidence: 99%

Acceleration-Based Study of Optimum Exercise for Human Weight-Bearing Bones Enhancement

et al. 2010

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Physical activity is known to enhance the mechanical competence of bones. However, p r e v i o u s r e l a t e d s t u d i e s p r o v i d e l i t t l e information regarding the optimum exercisesfor the prevention of osteoporosis in the lumbar region, including the hip joints. Physical activities can be evaluated by simultaneously c o l l e c t i n g a c c e l e r a t i o n d a t a f r o m accelerometers worn on different parts of the body. We attached three triaxial accelerometers to the ankle, knee, and lumbar points of 20 young women subjects in order to perform acceleration measurements while walking (a flat surface and a stairway) and jumping rope. The acceleration at the lumbar point while walking a flat surface, ascending stairs, and descending stairs were 1.96 ± 0.28, 1.92 ± 0.29, and 2.88 ± 0.66G, respectively. On the other hand, jumping rope provided an acceleration of 5.4G at the lumbar point. This value is higher than the threshold acceleration necessary to induce an osteogenic response in the lumbar region of young subjects, namely 3.5G. Descending stairs provided the second-highest intensity of acceleration in the lumbar region; however, it also produced excess impact acceleration at the ankle and knee points. Jumping rope, in contrast, provided sufficient intensity of acceleration in the lumbar region, but the acceleration imparted to the ankle and knee points remained at a tolerable level. Moreover, correlation analyses of the acceleration at the ankle, knee, and lumbar points during each physical activity showed that the knee joints solely regulated the strong landing impact force while the subjects were descending the flight of stairs. In comparison, while jumping rope, the ankle and hip joints regulated the large landing impact force cooperatively, without overloading themselves. Our results suggest that jumping rope can provide sufficient acceleration to induce an osteogenic response in the lumbar region without causing an overload to any joints. Thus, jumping rope is one of the most optimum exercises for bone mineral gain in the proximal femur and lumbar vertebrae regions in young subjects.

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“…The absorbance was converted into the amount of produced para-nitrophenol (pNP) using a standard curve for pNP. Detail methods were described in Suzuki et al [11].…”

Section: Effects Of Ohpahs and E 2 On Osteoclastic Activity In Culturmentioning

confidence: 99%

“…Then, the direct effect of 4-hydroxybenz[a]anthracene (4-OHBaA) on osteoclasts and osteoblasts in teleosts was examined using the assay system with fish scale, which contains osteoclasts, osteoblasts, and the bone matrix of two layers (bony layer: a thin, well-calcified external layer; a fibrillary layer: a thick, partially calcified layer) [11,12]. Using the scale assay, furthermore, we compared the results with those of 1-hydroxypyrene (1-OHPy), which had no binding activity to ER as a negative control.…”

Section: Introductionmentioning

confidence: 99%

Toxic effect of polycyclic aromatic hydrocarbon metabolites on fish bone metabolism

Hayakawa

Suzuki

Kitamura

et al. 2010

WIT Transactions on Ecology and the Environment

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Marine water is contaminated with polycyclic aromatic hydrocarbons (PAHs) from rivers and the atmosphere. Oil spilled from tankers or petroleum factories also causes marine pollution with PAHs. It is well known that PAHs, such as benzo [a]pyrene, show carcinogenicity and/or mutagenicity. In developing teleost fish, furthermore, it has been reported that spinal deformity was induced in Pacific herring and pink salmon by PAHs, although the detail mechanism of toxicity in teleosts is not elucidated yet. As a toxic pathway of PAHs in animals, the metabolic activation of PAHs in the presence of P450 is considered. In teleosts, as well as in mammals, it has been reported that PAHs are converted into monohydroxylated polycyclic aromatic hydrocarbons (OHPAHs) by an enzyme: cytochrome P4501A1. Thus, OHPAH might have a toxic effect in teleosts. In the present study, we examined the estrogenic activity of OHPAHs using the yeast-two hybrid assay system with human estrogen receptor ER) . As a result, we detected estrogenic activity in 4-hydroxybenz[a]anthracene (4-OHBaA). Then, the direct effect of 4-OHBaA on osteoclasts and osteoblasts in teleosts was examined using the assay system with fish scales, which contain osteoclasts, osteoblasts, and the bone matrix of two layers (bony layer: a thin, well-calcified external layer; a fibrillary layer: a thick, partially calcified layer). When the scales of goldfish and wrasse were incubated with 4-OHBaA, which showed agonistic activity in the yeast-two hybrid assay system described above, the suppressing effect was observed for both osteoclasts and osteoblasts. The osteoclastic and osteoblastic activities did not change with 1-hydroxypyrene, which has no binding activity to human ER. Thus, we conclude that PAH metabolites have a toxic effect on fish bone metabolism and that careful attention should be given to aquatic PAH contamination.

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Effect of vibration on osteoblastic and osteoclastic activities: Analysis of bone metabolism using goldfish scale as a model for bone

Cited by 45 publications

References 47 publications

Effects of Inorganic Mercury and Methylmercury on Osteoclasts and Osteoblasts in the Scales of the Marine Teleost as a Model System of Bone

Effects of Inorganic Mercury and Methylmercury on Osteoclasts and Osteoblasts in the Scales of the Marine Teleost as a Model System of Bone

Acceleration-Based Study of Optimum Exercise for Human Weight-Bearing Bones Enhancement

Toxic effect of polycyclic aromatic hydrocarbon metabolites on fish bone metabolism

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