Effect of very long chain fatty acids on peroxisomal β‐oxidation in primary rat hepatocyte cultures

Christiansen, Erling N.; Gray, Tim J.B.; Lake, Brian G.

doi:10.1007/bf02534779

Cited by 10 publications

(2 citation statements)

References 17 publications

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“…The high rate of oxidation of laurate in liver homogenates may be explained in part by its high peroxisomal b-oxidation as observed in our study but also by a high carnitineindependent mitochondrial b-oxidation as observed in isolated rat hepatocytes (Christensen et al 1989). The high peroxisomal oxidation rate of laurate may represent a metabolic adaptation of hepatocytes in response to an excessive uptake of FA by the liver (Christiansen et al 1985). The latter may be due, at least in part, to the higher digestibility and hence, metabolizable energy value of a milk-substitute rich in CO compared with that rich in TA (Table 1; Aurousseau et al 1984b).…”

Section: Hepatic Fatty Acid Oxidationsupporting

confidence: 68%

Effects of dietary coconut oil on fatty acid oxidation capacity of the liver, the heart and skeletal muscles in the preruminant calf

et al. 1999

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The oxidative capacity of the liver, the heart and skeletal muscles for fatty acids were investigated in preruminant calves fed for 19 d on a milk-replacer containing either coconut oil (CO, rich in 12:0) or tallow (rich in 16:0 and 18:1). Weights of the total body and tissues did not differ significantly between the two groups of animals but plasma glucose and insulin concentrations were lower in the CO group. Feeding on the CO diet induced an 18-fold increase in the hepatic concentration of triacylglycerols. Rates of total and peroxisomal oxidation of [1-14C]laurate, [1-14C]palmitate and [1-14C]oleate were measured in fresh tissue homogenates. Higher rates of total oxidation in liver homogenate and of peroxisomal oxidation in liver, heart and rectus abdominis muscle homogenates were observed with laurate used as substrate. Furthermore, the relative contribution of peroxisomes to total oxidation was 1·9-fold higher in the liver and in the heart with laurate than with oleate or palmitate. Finally, the peroxisomal oxidation rate of oleate was 1·5-fold higher in the hearts of calves fed on the CO diet. Whatever the tissue, citrate synthase (CS, EC 4.1.3.7) and cytochrome c oxidase (COX, EC 1.9.3.1) activities were similar between the two groups of calves but the COX : CS activity ratio was lower in the liver of the CO group. In conclusion, laurate is better catabolized by peroxisomes than long-chain fatty acids, especially in the liver. Elongation of lauric acid after partial oxidation might explain the hepatic triacylglycerol accumulation in calves fed on the CO diet.

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Section: Hepatic Fatty Acid Oxidationsupporting

confidence: 68%

Effects of dietary coconut oil on fatty acid oxidation capacity of the liver, the heart and skeletal muscles in the preruminant calf

et al. 1999

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“…Moreover, the increase in lactate dehydrogenase release was observed after 24 h incubation of hepatocytes with 0.32 mM of EA. Interestingly, a lower dose of EA (0.1 mM) revealed no effect on the activity of peroxisomal β-oxidation, carnitine acetyltransferase, or palmitoyltransferase [ 17 ]. Similarly, the compound not only increased β-oxidation activity in rat hepatocytes but was also cytotoxic to the cells, causing higher LDH leakage than in the untreated control cells [ 18 ].…”

Section: Resultsmentioning

confidence: 99%

Erucic Acid—Both Sides of the Story: A Concise Review on Its Beneficial and Toxic Properties

et al. 2023

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Erucic acid (EA) is monounsaturated fatty acid (22:1 n-9), synthesized in the seeds of many plants from the Brassicaceae family, with Brassica napus, B. rapa, or B. carinata considered as its richest source. As the compound has been blamed for the poisoning effect in Toxic Oil Syndrome, and some data indicated its cardiotoxicity to rats, EA has been for decades classified as toxic substance, the use of which should be avoided. However, the cardiac adverse effects of EA have not been confirmed in humans, and the experiments in animal models had many limitations. Thus, the aim of this review was to present the results of the so far published studies on both toxic, and pharmacological properties of EA, trying to answer the question on its future medicinal use. Despite the ambiguous and relatively small data on toxic and beneficial effects of EA it seems that the compound is worth investigating. Further research should be particularly directed at the verification EA toxicity, more in-depth studies on its neuroprotective and cytotoxic properties, but also its use in combination with other drugs, as well as its role as a drug carrier.

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Mechanisms of regulation of liver fatty acid-binding protein

Kaikaus¹,

Chan²,

Montellano³

et al. 1993

Cellular Fatty Acid-Binding Proteins II

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Effect of very long chain fatty acids on peroxisomal β‐oxidation in primary rat hepatocyte cultures

Cited by 10 publications

References 17 publications

Effects of dietary coconut oil on fatty acid oxidation capacity of the liver, the heart and skeletal muscles in the preruminant calf

Effects of dietary coconut oil on fatty acid oxidation capacity of the liver, the heart and skeletal muscles in the preruminant calf

Erucic Acid—Both Sides of the Story: A Concise Review on Its Beneficial and Toxic Properties

Mechanisms of regulation of liver fatty acid-binding protein

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