Effect of Vasoactive Intestinal Peptide (VIP) and Gastric Inhibitory Peptide (GIP) on Insulin and Glucagon Release by Perifused Newborn Rat Pancreas

Bataille, D.; Jarrousse, C.; Vauclin, N.; Gespach, Christian; Rosselin, G

doi:10.1007/978-1-4612-6366-1_19

Cited by 11 publications

(7 citation statements)

References 33 publications

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“…VIP has been localized in nerve terminals of the pancreatic islets of several species, including rats and humans (4, 5), and within intrapancreatic ganglia (5). VIP has also been shown to be insulinotropic both in vivo (20) and in vitro in the rat (8)(9)(10). Based on these findings, the present study was undertaken to ascertain the role of VIP in the development of the glucose-induced biphasic insulin release response.…”

Section: Resultsmentioning

confidence: 94%

“…In view of the incomplete effects of glucose on islet maturation, it should be noted that digestive and neural reflexes are initiated during the neonatal period, suggesting possible effects by gut hormones and/or neuropeptides. One such neuropeptide, vasoactive intestinal peptide (VIP), has been immunolocalized in nerves of rat islets (5-7) and has been found to stimulate insulin and glucagon release from both the adult (8,9) and newborn rat pancreas (10). It was therefore decided to investigate the effects of VIP on the glucose-induced insulin release pattern of cultured fetal rat islets.…”

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confidence: 99%

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Vasoactive Intestinal Polypeptide Enhances Hormone Content and Insulin Release in Cultured Fetal Rat Islets

Rowley

Fletcher

Dudek

et al. 1987

Experimental Biology and Medicine

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Section: Resultsmentioning

confidence: 94%

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confidence: 99%

Vasoactive Intestinal Polypeptide Enhances Hormone Content and Insulin Release in Cultured Fetal Rat Islets

Rowley

Fletcher

Dudek

et al. 1987

Experimental Biology and Medicine

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“…The role of GIP as physiologic incretin factor appears firmly established (1 1,12). GIP can potentiate the release of insulin from the perfused newborn and adult rat pancreas (13,14), monolayer cultures of neonatal rat pancreatic islet cells (15), and from isolated adult rat islets (16). Receptors for GIP have been characterized in membrane preparations from hamster p-cell tumors (17).…”

Section: Discussionmentioning

confidence: 99%

A Comparison of the Insulinotropic and the Insulin-Inhibitory Actions of Gut Peptides on Newborn and Adult Rat Islet Cells

Ishizuka

Singh²,

Greeley

et al. 1988

Pancreas

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The objective of this study was to examine the release of insulin from cultured islet cells, taken from the pancreas of newborn and adult rats, in response to gastric inhibitory polypeptide (GIP), cholecystokinin-8 (CCK-g), calcitonin gene-related peptide (CGRP), and pancreastatin. GIP ( lo-' M) potentiated glucose-stimulated release of insulin in a dose-dependent fashion from both newborn and adult islet cells. CCK-8 (>lo-* M) also increased glucose-stimulated release of insulin from newborn islet cells, however its effect was not significant and not as strong as that observed with adult islet cells. Culture of newborn islet cells for 3 weeks with media containing high concentrations of glucose (16.7 mM) enhanced insulin release in response to CCK-8. CGRP did not affect the release of insulin from newborn islet cells, whereas at M, it reduced the release of insulin from adult islet cells by 66 _t 4%. Pancreastatin ( loe9-M) did not affect the release of insulin from newborn islet cells when cells were incubated with 4.2 mM glucose, whereas it stimulated the release of insulin from adult islet cells in a dose-dependent fashion. When incubated with 16.7 mM glucose, pancreastatin inhibited the release of insulin from both newborn and adult islet cells. These results indicate that newborn islet cells experience developmental changes which render them responsive to enteric peptides. Key Words: Insulin-Islet cell-Gastric inhibitory peptide (GIP)-Cholecystokinin-8 (CCK-8)-Calcitonin gene-related peptide (CGRP)-Pancreastatin.The effects of aging on the release of insulin from the pancreas have been studied before, but most earlier studies have concentrated on glucosestimulated release of insulin and on a comparison of insulin secretion in young adult rodents with that of senescent rodents (1-3). Several gut peptides have either insulinotropic or insulin-inhibitory actions (4), however, whether their actions are modified by aging is not known. In this study, we compared the effect of the following gut peptides on the release of Manuscript received May 27, 1987; revised manuscript acinsulin from cultured newborn and adult rat islet cystokinin-8 (CCK-8), calcitonin gene-related peptide (CGRP), and pancreastatin. Pancreastatin is a new peptide that was extracted from the porcine pancreas by Tatemoto and colleagues (5,6); it con-

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“…We already mentioned these limitations of our procedure, in which blood is used as perfusate and recirculation is prohibited. 8 VIP CONTROL VIP was previously reported to stimulate the release of glucagon from dog, 16>17 cat, 18 and rat 19 pancreas, but the physiologic relevance of these observations remains unknown. The present investigation has shown that VIP, administered intra-arterially, induced its expected marked vasodilation in the isolated, perfused, canine stomach as in many other organs.…”

Section: Cholinergic Controlmentioning

confidence: 99%

Neurotransmitters and Glucagon Release From the Isolated, Perfused Canine Stomach

Lefèbvre

Luyckx

1980

Diabetes

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The isolated, perfused, canine stomach was used to investigate the effect of three neurotransmitters--norepinephrine, acetylcholine (or its analogue carbamylcholine), and VIP (vasoactive intestinal peptide)--on gastric glucagon release. Norepinephrine at the two concentrations tested (3.10-8 and 7.10-7 M) did not influence gastric glucagon release. In contrast, acetylcholine or carbamylcholine (5.10-6 M) as well as VIP (46-60 ng/ml) unequivocally stimulated gastric glucagon release, an effect apparently independent of the changes in blood flow. These results are in sharp contrast with the previously reported lack of effect of an electric stimulation of the vagus nerves on the release of glucagon from the dog stomach. An absence of innervation of the canine gastric A-cell would probably best explain this situation.

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Effect of Vasoactive Intestinal Peptide (VIP) and Gastric Inhibitory Peptide (GIP) on Insulin and Glucagon Release by Perifused Newborn Rat Pancreas

Cited by 11 publications

References 33 publications

Vasoactive Intestinal Polypeptide Enhances Hormone Content and Insulin Release in Cultured Fetal Rat Islets

Vasoactive Intestinal Polypeptide Enhances Hormone Content and Insulin Release in Cultured Fetal Rat Islets

A Comparison of the Insulinotropic and the Insulin-Inhibitory Actions of Gut Peptides on Newborn and Adult Rat Islet Cells

Neurotransmitters and Glucagon Release From the Isolated, Perfused Canine Stomach

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