Effect of vascular endothelial growth factor C (VEGF-C) gene transfer in rat model of secondary lymphedema

Li, Yanli; Fang, Yunhai; Dong, Ping; Gao, Jie; Liu, Rong; Hua, Tian; Ding, Zhaoxi; Bi, Yushun; Liu, Zhiyu

doi:10.1016/j.vph.2008.05.003

Cited by 21 publications

(20 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Previous small animal models have been described and investigated. 12,14–17 But, inconsistencies in results and methodology make these difficult to implement in the experimental setting. In addition, the quantification methods were inconsistent and inaccurate in previous studies.…”

Section: Discussionmentioning

confidence: 99%

“…In a previously reported lymphedema model, volume estimations were made by limb circumference measurements and water displacement volumetry, 12,15,17 but the studies showed high variability between control and the lymphedema areas. In another study, T2-weighted magnetic resonance imaging was also used for measuring the volume of the fluid accumulation in the subcutaneous tissue, but this methodology has significant associated costs.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Developing a Lower Limb Lymphedema Animal Model with Combined Lymphadenectomy and Low-dose Radiation

Yang¹,

Nguyen²,

Wu³

et al. 2014

Plastic and Reconstructive Surgery Global Open

View full text Add to dashboard Cite

Background:This study was aimed to establish a consistent lower limb lymphedema animal model for further investigation of the mechanism and treatment of lymphedema.Methods:Lymphedema in the lower extremity was created by removing unilateral inguinal lymph nodes followed by 20, 30, and 40 Gy (groups IA, IB, and IC, respectively) radiation or by removing both inguinal lymph nodes and popliteal lymph nodes followed by 20 Gy (group II) radiation in Sprague-Dawley rats (350–400 g). Tc99 lymphoscintigraphy was used to monitor lymphatic flow patterns. Volume differentiation was assessed by microcomputed tomography and defined as the percentage change of the lesioned limb compared to the healthy limb.Results:At 4 weeks postoperatively, 0% in group IA (n = 3), 37.5% in group IB (n = 16), and 50% in group IC (n = 26) developed lymphedema in the lower limb with total mortality and morbidity rate of 0%, 56.3%, and 50%, respectively. As a result of the high morbidity and mortality rates, 20 Gy was selected, and the success rate for development of lymphedema in the lower limb in group II was 81.5% (n = 27). The mean volume differentiation of the lymphedematous limb compared to the health limb was 7.76% ± 1.94% in group II, which was statistically significant compared to group I (P < 0.01).Conclusions:Removal of both inguinal and popliteal lymph nodes followed by radiation of 20 Gy can successfully develop lymphedema in the lower limb with minimal morbidity in 4 months.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Developing a Lower Limb Lymphedema Animal Model with Combined Lymphadenectomy and Low-dose Radiation

Yang¹,

Nguyen²,

Wu³

et al. 2014

Plastic and Reconstructive Surgery Global Open

View full text Add to dashboard Cite

show abstract

“…Commonly, a significant tissue mass is removed with attendant dissection of lymphatic vessels and nodes. Popular models include the hind limb models in dogs [12] and rats [13,14] , rabbit ear models [3,15] and rodent tail models [16][17][18] . In some animal models, radiation is applied in addition to surgical tissue resection [19,20] .…”

Section: Animal Models Of Lymphedemamentioning

confidence: 99%

Lymphedema Development and Lymphatic Function following Lymph Node Excision in Sheep

et al. 2009

View full text Add to dashboard Cite

show abstract

“…Anteriormente utilizados, modelos caninos eram complexos e seriam considerados antiéticos nos dias atuais (LÄHTEENVUO et al, 2011 (LIU et al, 2008;OASHI et al, 2012). Já em alguns cortes histológicos das regiões do membro pélvico dos animais do grupo tratado com VEGFC observa-se de maneira qualitativa, no tecido intermuscular, alguns vasos linfáticos de calibre maior que os encontrados nos grupos controle e cirurgia, sugerindo o efeito positivo da utilização do VEGFC na linfangiogênese (CHEUNG et al, 2006;LIU et al, 2008;OASHI et al, 2012;KUWAHARA et al, 2013).…”

Section: Transcrição Reversaunclassified

“…Já em alguns cortes histológicos das regiões do membro pélvico dos animais do grupo tratado com VEGFC observa-se de maneira qualitativa, no tecido intermuscular, alguns vasos linfáticos de calibre maior que os encontrados nos grupos controle e cirurgia, sugerindo o efeito positivo da utilização do VEGFC na linfangiogênese (CHEUNG et al, 2006;LIU et al, 2008;OASHI et al, 2012;KUWAHARA et al, 2013). Nakao et al (2013) JIN et al, 2009;OASHI et al, 2012;HÄGERLING et al, 2013;KUWAHARA et al, 2013).…”

Section: Transcrição Reversaunclassified

Tratamento com VEGFC para revascularização linfática em membros pélvicos de camundongos

Ferrão¹

View full text Add to dashboard Cite

Effect of vascular endothelial growth factor C (VEGF-C) gene transfer in rat model of secondary lymphedema

Cited by 21 publications

References 27 publications

Developing a Lower Limb Lymphedema Animal Model with Combined Lymphadenectomy and Low-dose Radiation

Developing a Lower Limb Lymphedema Animal Model with Combined Lymphadenectomy and Low-dose Radiation

Lymphedema Development and Lymphatic Function following Lymph Node Excision in Sheep

Tratamento com VEGFC para revascularização linfática em membros pélvicos de camundongos

Contact Info

Product

Resources

About