A fuel additive has been believed to raise engine performance by increasing fuel efficiency. However, a study about the influence of fuel additives on CO and CO2 emission has not been clearly understood yet. In this study, we investigate the influence of fuel additives on CO and CO2 motorcycle emission. Ten different additives: Bio additives and synthetic additives mixed with gasoline with RON of 90 used to operate a standard 100 cc motor engine. The additive concentration was determined by using the factory recommended dose. The engine was activated for 100 seconds during the smoke collection procedure. The smoke was collected into a measurement chamber with a dimension of (20 × 30 × 20) cm3. The CO and CO2 concentrations were measured by using Q-Trak Indoor Air Quality Monitor 7575. The remaining fuel was drained after the measurement to calculate the consumed one. In the result, the bio-additive produced CO2 of (0.44 × 103 - 2.76 × 103) ppm and synthetic additive of (0.12 × 103 - 1.48 × 103) ppm. On the other hand, the bio additive produced CO of (0.12 × 103-1.11 × 103) ppm while the synthetic produces (0.12 × 103-1.11 × 103) ppm. The bio and synthetic additive CO2 emission factors were calculated of (0.28–8.01) ppm/ml fuel and (0.88-13.17) ppm/ml. For CO, the emission factor was obtained of (0.48–8.93) ppm/ml and 0.29 ppm/ml -4.08 ppm/ml for bio and synthetic additive, respectively. In conclusion, using bio-additives produced more CO2 and CO emissions on the motorcycle.