Effect of various concentrations of lead nitrate on the induction of micronuclei in mouse bone marrow

Jagetia, Ganesh Chandra; Aruna, R

doi:10.1016/s1383-5718(98)00052-7

Cited by 24 publications

(7 citation statements)

References 29 publications

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“…The metallic elements found in the soils used in this study, a higher concentration of all the metals analyzed was found in soils influenced by the presence of the industrial complex compared with the control soil (As, Cd, Cr, Cu, Ni, Pb and Zn), Lead (Jagetia & Aruna, 1998) and nickel (Dhir, Agarwal, Sharma, & Talukder, 1991) are clastogenic agents that induce micronucleus formation. However, these are not the only metals that generate mutagenic effects because the studied soil presents a variety of metallic elements in varying degrees of contamination that mix in complex ways (SilvaJunior, Rocha, & Vargas, 2009).…”

Section: Resultsmentioning

confidence: 67%

<b>Mutagenic effect of contaminated soil on the offspring of exposed rats

Garcia

Silva

Muccillo-Baisch

2016

Acta Sci. Health Sci.

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ABSTRACT. This study aimed to measure irreversible DNA damage in the offspring of rats exposed to contaminated soil. Bone marrow cells were removed from the femurs of the offspring of rats that were exposed to contaminated soil via gavage during three different periods: 21 days prior to detection of pregnancy, during pregnancy and 21 days after birth, during the lactation period. For each group, we also had a control group under the same experimental conditions but exposed to uncontaminated soil. The results showed that pups borne of rats exposed to contaminated soil had an increase in the number of micronuclei in the different exposure groups compared with their respective control groups. This suggests a potential risk of mutagenic damage to the offspring of pregnant women who live at the sampling site.Keywords: soil contamination, reproduction, wistar rats, metals, micronucleus.Efeito mutagênico da exposição a solos contaminados sobre a prole RESUMO. Este estudo objetivou medir os danos irreversíveis de DNA na prole de ratas expostas a um solo contaminado, através do teste do micronúcleo. O mesmo foi realizado na medula óssea retirada do fêmur de filhotes de ratas expostas, via gavagem, em três diferentes períodos, ratas expostas durante 21 dias antes da detecção da prenhez, durante a prenhez e 21 dias após o nascimento, durante o período de aleitamento. Para cada grupo foi utilizado um grupo controle, mantido nas mesmas condições experimentais e expostos a um solo não contaminado. Os resultados obtidos nos filhotes expostos ao solo contaminado mostraram um aumento no número de micronúcleos nos três diferentes grupos expostos quando comparados aos seus respectivos grupos controle. Assim, os dados sugerem um potencial risco de dano mutagênico aos descendentes das gestantes residentes no local de amostragem.Palavras-chave: contaminação terrestre. reprodução. ratas wistar. elementos metálicos. micronúcleo.

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Section: Resultsmentioning

confidence: 67%

<b>Mutagenic effect of contaminated soil on the offspring of exposed rats

Garcia

Silva

Muccillo-Baisch

2016

Acta Sci. Health Sci.

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“…2 . In another study, Swiss albino rats were treated with 0.625-80 mg kg -1 bw Pb(NO 3 ) 2 and a significant increase in MN frequency of polychromatic erythrocyte cells was reported [20]. MN formation is an effective parameter used to evaluate the cytotoxic effects of chemicals and indicates cell division errors, spindle damages and chromosome breaks [21].…”

Section: A Effects On Body and Organ Weightmentioning

confidence: 99%

In Vivo Protective Role of Nigella sativa L. Against Pb(NO3)2 Induced Toxicity

Yapar

Çavuşoğlu

Yalçın

et al. 2019

Düzce Üniversitesi Bilim Ve Teknoloji Dergisi

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In this study, the protective role of Nigella sativa L. seed extract (NSSE) against Pb(NO 3 ) 2 toxicity in albino mice was investigated. For this purpose, the mice were randomly divided to six groups. In control group, mice were treated with tap water and in the treatment groups 500 mg kg -1 bw Pb(NO 3 ) 2 , 300 and 600 mg kg -1 bw doses of NSSE were exposed to mice. The effects of all treatments on organism development were investigated by determining the changes in body, liver and kidney weights of each group. Genotoxic effects were determined by investigating the chromosomal abnormalities (CAs) in bone marrow cells, micronucleus (MN) frequency in erythrocyte and buccal mucosa cells. As a result, it was observed that Pb(NO 3 ) 2 treatment resulted in a 6.33-fold decrease in body weight gain and 2.08 and 1.65-fold increase in liver and kidney weights compared to control group, respectively. In addition, it was determined that these alterations in weights were positively changed after NSSE treatment. From MN assays it was observed that MN frequencies of erythrocyte and buccal mucosa cells in 500 mg kg -1 bw Pb(NO 3 ) 2 treated group were found as 58.17±5.46 and 19.00±2.76 while in 600 mg kg -1 bw NSSE+Pb(NO 3 ) 2 treated group, the frequencies were determined as 32.67±3.78 and 6.50±1.87, respectively. A similar protective role was also observed against CAs formation, and 600 mg kg -1 NSSE treatment was found to reduce the chromatid breaks by 44%. As a result, it has been determined that NSSE can be used as a protective nutrient against the harmful effects of chemicals such as heavy metals especially Pb.

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“…Jagetia and Aruna (1998) reported that lead induced micronuclei were observed even at very low doses. After i.p.…”

Section: Pb (No 3 ) 2 Clastogenicitymentioning

confidence: 99%