Effect of various bud induction treatments on elongation and rooting of adventitious shoots of Canary Island pine (Pinus canariensis)

Pulido, Carolina Martínez; Harry, Indra S.; Thorpe, Trevor A.

doi:10.1007/bf00035974

Cited by 15 publications

(2 citation statements)

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“…Further testing on the performance of the rooted explants in the greenhouse will determine if the best treatments for in vitro rooting lead to the best acclimatisation rates or, on the contrary, the explants rooted with IBA show a better ex vitro performance as observed in radiata pine zygotic embryos (Montalbán 2011). IBA is the auxin currently used for rooting of cuttings of P.radiata and has been used in in vitro rooting protocols of P. canariensis (Martínez Pulido et al 1994) and P. heldreichii (Stojičić et al 1999).…”

Section: Discussionmentioning

confidence: 99%

A combined pathway of somatic embryogenesis and organogenesis to regenerate radiata pine plants

Montalbán

Diego

Igartua

et al. 2011

Plant Biotechnol Rep

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This study describes for the first time in Pinus genus a plant regeneration system via a combined pathway of somatic embryogenesis and organogenesis from immature seeds of radiata pine. Somatic embryos were obtained from embryogenic line 2162 of Pinus radiata D. Don on EDM basal medium containing 60 lM ABA and 6% sucrose. The explants used for organogenesis experiments were either freshly collected somatic embryos or somatic embryos germinated for 1 week. Germination medium was half-strength LP medium, supplemented with 0.2% activated charcoal. Different induction periods and BA concentrations were assayed for shoot induction. After induction treatments, explants were elongated on the same medium used for germination stage. Rooting medium was quarter-strength LP medium supplemented with three different auxin treatments: 1.5 mg L -1 1-naphthalene acetic acid (NAA), 1.5 mg L -1 indole-3-butyric acid (IBA) and 1 mg L -1 IBA with 0.5 mg L -1 NAA (MIX). The effect of the photon flux (120 mmol m -2 s -1 and darkness) in the first week of the explants in the rooting media was also tested. This methodology could offer an alternative to overcome some problems associated with somatic embryogenesis such as the seasonality of embryogenic tissue (ET) initiation or a low embryo production from the ET, a particularly important issue in the case of genetically transformed ETs.

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Section: Discussionmentioning

confidence: 99%

A combined pathway of somatic embryogenesis and organogenesis to regenerate radiata pine plants

Montalbán

Diego

Igartua

et al. 2011

Plant Biotechnol Rep

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“…The results were in accordance with that observed in Pinus canariensis C.Sm. (Pinaceae) by Pulido et al [15], wherein kinetin influenced shoot elongation. Different concentrations and combinations of cytokinins (BAP, TDZ, and Kn) were attempted to observe the synergistic effect on multiple shoot production.…”

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confidence: 99%

In Vitro Rapid Multiplication Protocol for Ex Situ Conservation of the Rare, Endemic Medicinal Plant Achyranthes coynei

et al. 2016

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!Achyranthes coynei is a rare, endemic species with potential medicinal properties. The present study aimed to develop an efficient in vitro rapid multiplication protocol for A. coynei. It was successfully propagated in vitro using apical shoot tips as explants. A combination of 6-benzylaminopurine (3.0 mg · L −1 ), thidiazuron (0.5 mg · L −1 ), and kinetin (0.5 mg · L −1 ) in MS basal proved best for multiple shoot induction, yielding 4.8 shoots per explant after 60 days of culture. The shoots were rooted with a frequency of 80% after 30 days of culture on MS agar medium fortified with IBA/NAA (0.5 mg · L −1 ) before being acclimatized in the greenhouse. The developed protocol will serve as a tool to restore the rare status of the plant and will append its sustainable utilization. Key wordsAchyranthes coynei · Amaranthaceae · in vitro · rapid multiplication · kinetin , 3-5]. The plant is used in the treatment of various ailments by traditional practitioners of the region [1,6]. The plant has been scientifically studied for its antimicrobial and antioxidant activities, correlating its medicinal properties with the phytoconstituents present [6][7][8][9][10][11].A. coynei is a perennial, profusely branching shrub growing uneven on road sides and on canal bunds along with Achyranthes aspera L. (l " Fig. 1 a) [1]. Fast erosion of medicinal plants has been reported from the study region [12,13]. Parameters such has deforestation, reduced rainfall, dam construction, grazing, fire wood collection, and expansion of cultivation land are hindering the natural habitat and population of medicinal plants including A. coynei [1, 12, 13]. The plant needs immediate attention to retain its medicinal properties displayed by the presence of richer triterpenoids over A. aspera [2,6,11]. The endemic and rare status of the plant by virtue of its assorted distribution makes it a vulnerable component of the community. And as quoted, endemic species confined to threatened ecosystems will certainly hit by extinction episodes and are most in need of rapid and effective conservation action [1,14]. Under these circumstances there is a need to facilitate an alternative strategy for its conservation and sustainable use. An in vitro system provides such an alternative tool. To the best of the authors knowledge, no work has been carried out on the in vitro multiplication of this rare, medicinal plant. Thus, the study was taken up to develop an efficient protocol for its rapid multiplication and ex situ conservation. Germination studies (1 to 30 day) showed 70% seed germination, and the first seed germinated on 7th day. The germination was higher in seeds deprived of a persistent calyx (80%) compared to seeds bearing a calyx (60 %).Higher numbers of multiple shoots were observed after 4 weeks of inoculation on BAP (3.0 mg · L −1 ), TDZ (0.5 mg · L −1 ), and Kn (1.5 mg · L −1 ) added individually to MS basal (l " Table 1). The shoot length was below 1 cm in BAP 3.0 mg · L −1 and TDZ 0.5 mg · L −1 (l " Fig. 1 b, c), whereas Kn 1.5 mg · L...

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Aseptic ectomycorrhizal synthesis between Abies firma and Cenococcum geophilum in artificial culture

et al. 2000

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A simple in vitro system is described for the synthesis of Abies firma-Cenococcum geophilum ectomycorrhizas. Sterilized A. firma seedlings on both MMN and FH media were inoculated with hyphal discs from actively growing margins of C. geophilum colonies. Typical ectomycorrhizas formed on seedlings on FH medium after 3 mo of incubation. By light microscopy, the synthesized mycorrhizas were seen to possess a thin mantle from which emanated extraradicle hyphae and highly branched, rarely septate intracortical Hartig net mycelium, characteristic ectomycorrhizal features. This is the first report of aseptic ectomycorrhization of A. firma seedlings by C. geophilum. This model system will facilitate detailed studies on ectomycorrhizal development of Abies species.

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Effect of various bud induction treatments on elongation and rooting of adventitious shoots of Canary Island pine (Pinus canariensis)

Cited by 15 publications

References 10 publications

A combined pathway of somatic embryogenesis and organogenesis to regenerate radiata pine plants

A combined pathway of somatic embryogenesis and organogenesis to regenerate radiata pine plants

In Vitro Rapid Multiplication Protocol for Ex Situ Conservation of the Rare, Endemic Medicinal Plant Achyranthes coynei

Aseptic ectomycorrhizal synthesis between Abies firma and Cenococcum geophilum in artificial culture

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