Effect of various antagonists on theChanna striatusfillet extract antinociception in mice

Abstract

The effects of an aqueous supernatant of haruan (ASH) (Channa striatus) fillet extract on various antinociception receptor system activities were examined using a mouse abdominal-constriction model. Mice that were pretreated with distilled water, s.c., followed 10 min later by administration of 25%, 50%, and 100% concentration ASH, s.c., produced a significant concentration-dependent antinociceptive activity (p < 0.001). Pretreatment with naloxone (0.3, 1.0, and 3.0 mg/kg body mass), 10 min before ASH administration, failed to block the extract antinociception. Pretreatment of the 100% concentration ASH with mecamylamine (5 mg/kg), pindolol (10 mg/kg), and haloperidol (1 mg/kg) also did not cause any significant change in its antinociception. However, pretreatment with atropine (5 mg/kg), bicuculline (10 mg/kg), phenoxybenzamine (10 mg/kg), and methysergide (5 mg/kg) were found to reverse ASH antinociception. Based on the above findings, the ASH is suggested to contain different types of bioactive compounds that act synergistically on muscarinic, GABAA, alpha-adrenergic, and serotonergic receptor systems to produce the observed antinociception.

Antinociceptive, Anti-inflammatory and Antipyretic Effects of <i>Solanum nigrum</i> Chloroform Extract in Animal Models

“…27,28) Experimental Animals Male Balb/C mice (20 -30 g) and Sprague-Dawley rats (200-300 g) were obtained from the Institute of Medical Research, Kuala Lumpur, Malaysia. The animals were transported to the Animal Unit of Faculty of Veterinary, Universiti Putra Malaysia (UPM), Serdang, Selangor, Malaysia and acclimatized for 1 week before used.…”

Effects of various receptor antagonists on the peripheral antinociceptive activity of aqueous extracts of Dicranopteris linearis, Melastoma malabathricum and Bauhinia purpurea leaves in mice

“…The abdominal constrictions seen are due to the acetic acid-induced irritation of the peritoneal cavity (Deraedt et al, 1980). In order to elucidate the mechanisms of antinociceptive activities of compounds/extracts, particularly the types of receptors that modulate, various receptor antagonists were pre-challenged with the compounds/extracts (Omar et al, 2006;Zakaria et al, 2005). A receptor antagonist is a drug that does not incite a biological response itself once it binds to a receptor, but blocks agonist-mediated responses (Katzung et al, 2012).…”

“…The three extracts (300, 500, and 1000 mg/kg), DH 2 O (distilled water; vehicle) and acetylsalicylic acid (ASA; positive control) were administered subcutaneously (s.c.) into the mice (n = 11) and 30 min later, the animals were subjected to the 0.6% acetic acid-induced abdominal constriction test (Zakaria et al, 2008(Zakaria et al, , 2006(Zakaria et al, , 2007. To determine the possible mechanisms of antinociception, thirteen groups of animals were used and each extract, only at 500 mg/kg, was pre-challenged with various receptors' antagonists or mediators' blockers as described elsewhere (Abdel-Salam, 2007;Alhaider, 1991;Ghelardini et al, 2000;Zakaria et al, 2005;Zarrindast et al, 1994).…”

“…To determine the role of opioid and non-opioid receptors and pain mediator blockers in the modulation of the antinociceptive activities of AEDL, AEMC and AEBP, a separate procedure as described by Zakaria et al (2005) was adopted with slight modifications. Thirteen groups of animals (n = 11) were pretreated (i.p.)…”

Gastroprotective Effect of Freeze Dried Stripped Snakehead Fish (Channa striataBloch.) Aqueous Extract against Aspirin Induced Ulcerogenesis in Pylorus Ligated Rats

“…Mohd Shafri and Mat Jais reviewed in detail the description, habitat, traditional therapeutic uses, chemical composition, and scientifically proven medicinal properties [6]. The fish is reported to possess antinociceptive [7, 8], antipyretic [9], antidepressant [10, 11], anti-inflammatory [12], antifungal [13], antimicrobial [14], antiosteoarthritic [15], neuroregenerative, and restorative [16] properties and enhances wound healing process [17–19]. In view of traditional and scientific reports on wound healing potential of C. striata and presence of wide range of bioactive components, the present study was undertaken to investigate antiulcer activity.…”