Effect of vacuum sealing drainage on the expression of VEGF and miRNA-17-5p in seawater-immersed blast-injury wounds

Abstract

Abstract. The aim of the present study was to compare and observe the expression levels of vascular endothelial growth factor (VEGF) and miRNA-17-5p during the treatment of seawater-immersed blast-injury wounds (SIBIW) under different conditions of vacuum sealing drainage (VSD), and to identify the optimized range of VSD treatment and partially explain its mechanisms. The bilateral hips and scapulae of experimental pigs (weight, 25-30 kg) were subjected to blast-injury wounds, followed by the seawater immersion. The animals then underwent conventional dressing treatment under 120, 180 and 240 mmHg VSD. Visual observation, in addition to histological, immunohistochemical and molecular biological techniques were applied to compare and observe the extent of wound healing and expression levels of VEGF and miRNA-17-5p. The wound healing of the VSD treatment group was improved compared with the control group, with 120 mmHg negative pressure producing the most marked effect. miR-17-5p expression was detected in the SIBIW granulation tissues. There was significant difference between each VSD treatment group and control group at each time point (P<0.05). Thus, the present results show that miR-17-5p can be expressed in SIBIW granulation tissues, and this effect is most evident under 120 mmHg negative pressure, which may inform the optimized negative range for the treatment of SIBIW.

Effect of whole course seamless nursing mode on patients with chronic infectious wounds

“…It is clinically recommended to perform active therapies for chronic infectious wounds, such as dressing change treatment and vacuum sealing drainage 13 . However, insufficient knowledge and poor psychological state of patients may cause poor therapeutic effects 14 - 16 . Therefore, it is crucial to perform reasonable nursing interventions for such patients.…”

A meta‐analysis on the efficacy of vacuum sealing drainage combined with autologous platelet‐rich plasma in the treatment of Grade 2 and Grade 3 diabetic foot ulcers

“…It can also promote the production and release of growth‐promoting factors and accelerate the growth of granulation tissue. 31 , 32 , 33 In recent years, autologous PRP has been gradually used in the treatment of DFU and has achieved good results. 34 Autologous platelet‐rich gel belongs to the natural biological preparation of platelets, which is derived from the patient's blood and therefore has the advantage of low immunogenicity.…”

“…Directed VSD could effectively reduce wound tissue oedema, improve wound microcirculation, and remove harmful inflammatory molecules in time. It can also promote the production and release of growth‐promoting factors and accelerate the growth of granulation tissue 31‐33 . In recent years, autologous PRP has been gradually used in the treatment of DFU and has achieved good results 34 .…”

Human Adipose-Derived Stem Cells Promote Seawater-Immersed Wound Healing by Activating Skin Stem Cells via the EGFR/MEK/ERK Pathway

“…First, we established a full-thickness skin defect model and immersed the wound completely in artificial seawater. During the healing period on days 7 and 14, the reepithelialization and closure of the seawater-immersed wounds were slower than what was observed in control wounds, demonstrating that seawater impedes wound healing [21]. Subsequently, hADSCs or an equal volume of DMEM were injected around the wounds, and it was observed that wounds with the hADSC injection had a better closure efficiency.…”