Effect of Vaccine, Route, and Schedule on Antibody Response of Rabbits to Pasteurella tularensis

Nutter, John E.

doi:10.1128/aem.17.3.355-359.1969

Cited by 6 publications

(4 citation statements)

References 3 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Two studies evaluated aspects of LVS inoculation of the New Zealand white rabbit 71, 72 . Rabbits survived LVS inoculation given percutaneously or subcutaneously (10 5 organisms) and extended survival after SCHU S4 challenge by 3 days compared to nonvaccinated SCHU S4 challenged rabbits.…”

Section: Rabbit Modelsmentioning

confidence: 99%

Animal Models of Francisella tularensis Infection

Lyons

2007

Annals of the New York Academy of Sciences

View full text Add to dashboard Cite

The increased incidence of emerging infections has caused a resurgence in the development of animal models in order to study their pathophysiology and develop therapeutics against them. Optimizing these models and improving our ability to extrapolate information from animals to humans is critical because in many cases the animal model will represent the only modality for efficacy testing. Francisella tularensis (F. tularensis) is an emerging pathogen that fits this category. While there is a significant body of literature that has examined infections with F. tularensis in a variety of species, the optimal small animal model has yet to be defined. A vast majority of studies have used two strains of F. tularensis, the more virulent type A strain commonly found in North America and the less virulent type B strain common to Europe. None of the small animal models described in the literature thus far behave in a fashion identical to humans with respect to their sensitivity to SCHU S4 (type A) or live vaccine strains (LVS) (attenuated type B) and an ability of LVS vaccination to consistently protect against a SCHU S4 aerosol challenge, suggesting that significant work on animal model development still remains. This report briefly describes the parameters important for animal model development and reviews the literature related to animal models of F. tularensis, including the human model, and the characterization performed for those models.

show abstract

Section: Rabbit Modelsmentioning

confidence: 99%

Animal Models of Francisella tularensis Infection

Lyons

2007

Annals of the New York Academy of Sciences

View full text Add to dashboard Cite

show abstract

“…F *A comparative study of antigens extracted from Pasteurella tularensis by various chemical procedures has not been reported. Viable and killed whole-cell vaccines usually have been employed for producing antisera in animals (2,4,6). A few studies on antigenic extracts have been reported, but the emphasis was on immunogenicity (5,7) or their utility as a serological reagent for assessing preformed antibody (1,2,6,12).…”

mentioning

confidence: 99%

Antigens of Pasteurella tularensis: Preparative Procedures

Nutter

1971

Applied Microbiology

Self Cite

View full text Add to dashboard Cite

Ether-water (EW) extraction of Pasteurella tularensis produced better antigens than five other chemical procedures. EW extracts produced from stationary-phase, liquid-grown, saline suspensions of strain SCHU S4 cells regularly induced agglutinin and precipitin formation in rabbits. Mice, guinea pigs, and monkeys also responded to EW extracts but with lower antibody levels. The use of strains of lower virulence, acetone-dried cells, organisms grown on a solid medium, and abbreviated extraction conditions all resulted in extracts with a diminished antigenicity, but logarithmicphase and stationary-phase cells yielded equivalent EW extracts. The use of adjuvant, hyperimmunization, and large doses of antigen increased the precipitin responses of rabbits without appreciably altering the agglutinin response. By the appropriate combination of centrifugal fractionation of EW extracts, use of adjuvant, and vaccination schedule, rabbit antisera with either predominantly agglutinating or precipitating activities were obtained.

show abstract

“…Previous studies (4) demonstrated that shortterm hyperimmunization with P. tularensis LVS would increase the P. tularensis agglutinin titers of rabbits. This procedure was combined with that of sequential immunization used in the current studies.…”

Section: Resultsmentioning

confidence: 95%

Agglutinin Response of Rabbits to Combined Pasteurella tularensis-Brucella abortus Vaccination 1

Nutter¹

1970

Applied Microbiology

Self Cite

View full text Add to dashboard Cite

A schedule was developed for the simultaneous production of a Pasteurella tularensis-Brucella abortus antiserum in rabbits. Three doses of 101 viable P. tularensis LVS organisms were given intravenously at weekly intervals. One day prior to the final dose of P. tularensis, the rabbits received 109 viable cells of B. abortus strain 19 intravenously. The use of live vaccines, administered in this sequence, resulted in high agglutinin titers within 3 weeks. The maximal agglutinin titer to either organism was observed 1 week after the final injection. A single antiserum capable of reacting with more than one bacterium could facilitate serological testing and studies by serving as a screening reagent. A procedure was developed for the production of anti-Pasteurella tularensis antisera in rabbits by using the attenuated vaccine strain LVS (4). The purpose of the present study was to determine the feasibility of producing a bivalent antiserum by combined vaccination of rabbits with viable Brucella abortus strain 19 and P. tularensis LVS. MATERIALS AND METHODS Animals. New Zealand white rabbits weighing between 1.8 and 2.5 kg were used. Except where noted, all experimental groups contained five animals. Vaccines. The production and administration of viable tularemia vaccine have been reported (4). Desiccated B. abortus strain 19 vaccine was obtained

show abstract

Effect of Vaccine, Route, and Schedule on Antibody Response of Rabbits to Pasteurella tularensis

Cited by 6 publications

References 3 publications

Animal Models of Francisella tularensis Infection

Animal Models of Francisella tularensis Infection

Antigens of Pasteurella tularensis: Preparative Procedures

Agglutinin Response of Rabbits to Combined Pasteurella tularensis-Brucella abortus Vaccination 1

Contact Info

Product

Resources

About