Effect of ursodeoxycholic acid on inflammatory infiltrate in gallbladder muscle of cholesterol gallstone patients

Carotti, Simone; Guarino, Michele Pier Luca; Cicala, Michele; Perrone, Giuseppe; Alloni, Rossana; Segreto, Francesco; Rabitti, Carla; Morini, Sergio

doi:10.1111/j.1365-2982.2010.01510.x

Cited by 26 publications

(18 citation statements)

References 39 publications

(60 reference statements)

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“…Similar to our previous report, MC density was increased in the lamina propria and the muscularis mucosae in both CL and BD patients. For CL patients, this is similar to findings previously reported in adults with CL which demonstrated increased MC density and % of degranulated MCs in the muscular layer of adults with gallstones as compared to controls [16]. Unlike our previous report, MCs in the lamina propria were significantly increased in patients with CL as compared to patients with BD.…”

Section: Discussionsupporting

confidence: 90%

Mast cell activation and clinical outcome in pediatric cholelithiasis and biliary dyskinesia

et al. 2011

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BackgroundThe current study was undertaken to determine the degree of activation of gallbladder mucosal mast cells, whether mast cell (MC) density or activation differ between patients with and without a positive clinical response to cholecystectomy, and whether either density or activation correlate with gallbladder emptying.ResultsFifteen biliary dyskinesia (BD) and 13 symptomatic cholelithiasis (CL) patients undergoing cholecystectomy were prospectively enrolled. Gallbladder wall MC density (by immunohistochemistry) and activation (by electron microscopy) were determined. Clinical response was evaluated 30 days post-cholecystectomy on a 5-point Likert-type scale. A complete or nearly complete clinical response was seen in 100% of CL and in 87% of BD patients. The overall degranulation indices were 49.4 ± 18.7% for CL patients and 44.2 ± 16.8% for BD patients. Neither MC density nor activation correlated with the gallbladder ejection fraction. A complete clinical response was associated with lower epithelial MC density.ConclusionCholecystectomy is efficacious in relieving pain in both CL and BD patients. BD and CL are associated not only with increased MC density but a moderate to high degree of MC activation. A possible relationship between MC density and outcome for BD warrants further investigation.

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Section: Discussionsupporting

confidence: 90%

Mast cell activation and clinical outcome in pediatric cholelithiasis and biliary dyskinesia

et al. 2011

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“…[1] Carotti and coworkers showed higher number of CD68-positive monocytes/macrophages, granulocytes, mast cells, and inducible nitric oxide synthase positive cells and higher level of cyclooxygenase-2 enzyme in the muscle layer of gallstone patients compared with controls. [35]…”

Section: Discussionmentioning

confidence: 99%

Evaluation of paraoxonase, malondialdehyde, and lipoprotein levels in patients with asymptomatic cholelithiasis

Atamer

Kurdaş-Övünç

Yeşıl

et al. 2014

Saudi J Gastroenterol

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Background/Aim:To compare lipoprotein and malondialdehyde levels and paraoxonase-1 activity between subjects with asymptomatic cholelithiasis and controls.Patients and Methods:Eighty subjects with asymptomatic cholelithiasis (55 women, 25 men, mean age: 51, SD 14 years) and 40 control subjects without cholelithiasis (25 women, 25 men, mean age: 51, SD 12 years) were enrolled to the study. Serum paraoxonase activity, lipoproteins, and malondialdehyde were measured.Results:In the cholelithiasis group, serum total cholesterol, low-density lipoprotein cholesterol, and malondialdehyde were significantly higher and high-density lipoprotein cholesterol (HDL-C) and paraoxonase-1 were significantly lower than the controls. In cholelithiasis patients with serum glucose level > 100 mg/dL, body mass index, serum total cholesterol, triglyceride (TG), and malondialdehyde levels were significantly higher than cholelithiasis patients with serum glucose level < 100 mg/dL. Paraoxonase-1 activity was significantly lower in patients with serum glucose level > 100 mg/dL. In cholelithiasis patients with TG > 150 mg/dL, mean age, body mass index, glucose, total cholesterol, and malondialdehyde were significantly higher than in cholelithiasis patients with TG < 150 mg/dL. In cholelithiasis subgroup with TG > 150 mg/dL, HDL-C level and paraoxonase-1 activity were lower than in the cholelithiasis subgroup with TG < 150 mg/dL. All of the above comparisons were statistically significant (P < 0.05).Conclusions:Patients with asymptomatic cholelithiasis have evidence of increased lipid peroxidation and decreased antioxidant capacity. Patients with asymptomatic cholelithiasis with components of the metabolic syndrome have more lipid peroxidation and less antioxidant capacity than patients with asymptomatic cholelithiasis but without the components of the metabolic syndrome.

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“…This possible paradigm shift needs to be explored further by comparing smooth muscle function in control mice to Rag2 -/- mice fed the LD or to mice fed a lithogenic diet while being treated with an inhibitor of acute sterile inflammation such as a COX-2 antagonist. Interestingly, treatment of patients with symptomatic cholesterol gallstone with the hydrophilic bile salt ursodeoxycholic acid (UDCA) prior to cholecystectomy improves both contractility and inflammatory scores, possibly by decreasing cholesterol content of the plasma membrane (44-46). Furthermore, UDCA can prevent the muscle dysfunction caused by the hydrophobilic bile chenodeoxycholic acid (CDCA) in animal model and isolated cells (47, 48).…”

Section: Discussionmentioning

confidence: 99%

Disruption of gallbladder smooth muscle function is an early feature in the development of cholesterol gallstone disease

Lavoie

Nausch

Zane

et al. 2012

Neurogastroenterology Motil

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Background Decreased gallbladder smooth muscle (GBSM) contractility is a hallmark of cholesterol gallstone disease, but the interrelationship between lithogenicity, biliary stasis and inflammation are poorly understood. We studied a mouse model of gallstone disease to evaluate the development of GBSM dysfunction relative to changes in bile composition and the onset of sterile cholecystitis. Methods BALB/cJ mice were fed a lithogenic diet for up to 8 weeks, and tension generated by gallbladder muscle strips was measured. Smooth muscle Ca2+ transients were imaged in intact gallbladder. Key Results Lipid composition of bile was altered lithogenically as early as one week, with increased hydrophobicity and cholesterol saturation indexes; however, inflammation was not detectable until the fourth week. Agonist-induced contractility was reduced from weeks 2 through 8. GBSM normally exhibits rhythmic synchronized Ca2+ flashes, and their frequency is increased by carbachol (3μM). After one week, lithogenic diet-fed mice exhibited disrupted Ca2+ flash activity, manifesting as clustered flashes, asynchronous flashes or prolonged quiescent periods. These changes could lead to a depletion of intracellular Ca2+ stores, which are required for agonist-induced contraction, and diminished basal tone of the organ. Responsiveness of Ca2+ transients to carbachol was reduced in mice on the lithogenic diet, particularly after 4-8 weeks, concomitant with appearance of mucosal inflammatory changes. Conclusions & Inferences These observations demonstrate that GBSM dysfunction is an early event in the progression of cholesterol gallstone disease and that it precedes mucosal inflammation.

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Effect of ursodeoxycholic acid on inflammatory infiltrate in gallbladder muscle of cholesterol gallstone patients

Cited by 26 publications

References 39 publications

Mast cell activation and clinical outcome in pediatric cholelithiasis and biliary dyskinesia

Mast cell activation and clinical outcome in pediatric cholelithiasis and biliary dyskinesia

Evaluation of paraoxonase, malondialdehyde, and lipoprotein levels in patients with asymptomatic cholelithiasis

Disruption of gallbladder smooth muscle function is an early feature in the development of cholesterol gallstone disease

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