Effect of urogastrone on gastric secretion and plasma gastrin levels in normal subjects.

Elder, J. B.; Ganguli, P. C.; Gillespie, Iain; Gerring, E. L.; Gregory, H.

doi:10.1136/gut.16.11.887

Cited by 101 publications

(28 citation statements)

References 9 publications

(7 reference statements)

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“…A transformant E. coli strain, YK537(pTA1522), was cultivated in 1.2 liters of TG+1 medium for 6 hr at 37°C. Cells were harvested and treated with 100 ml of osmotic-shock buffer and then with 100 ml of cold water (16). (a) Lyophilized products were dissolved in 6 ml of 25 mM ammonium acetate (pH 5.8), applied to a Sephadex G-50 column (2.5 x 90 cm; particle size, 50-150 ,um), and eluted with the same buffer.…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Synthesis and secretion of human epidermal growth factor by Escherichia coli.

Oka¹,

Sakamoto²,

Miyoshi³

et al. 1985

Proc. Natl. Acad. Sci. U.S.A.

145

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A synthetic gene for human epidermal growth factor (hEGF) was joined to a sequence encoding the signal peptide of Escherichia coli alkaline phosphatase. This hybrid gene was placed under the control of the alkaline phosphatase gene (phoA) promoter in a recombinant plasmid, which was used to transfect E. coli. The hybrid protein that was expressed in host cells under conditions of phosphate limitation was processed accurately during the secretion process, and mature hEGF was recovered in the periplasmic fraction. On the other hand, no EGF was detected in the periplasmic space when the synthetic hEGF gene was not accompanied by the phoA signal sequence.

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Section: Resultsmentioning

confidence: 99%

“…It is a potent inhibitor of gastric acid secretion (15) and promotes epithelial cell proliferation both in vitro and in vivo (16 (18). Synthetic oligodeoxynucleotides were prepared by a solid-phase method (19).…”

mentioning

confidence: 99%

Synthesis and secretion of human epidermal growth factor by Escherichia coli.

Oka¹,

Sakamoto²,

Miyoshi³

et al. 1985

Proc. Natl. Acad. Sci. U.S.A.

145

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“…Elder et al, suggested that EGF acts at the parietal cell in the epithelium of the stomach (29), and Forgue-Lafitte et al demonstrated biochemically that there was high 1251 binding in gastric glands isolated from antral and fundic parts of the guinea pig stomach (34). Because gastric glands have heterogeneous cell types, more precise morphologic study is needed.…”

Section: Discussionmentioning

confidence: 99%

“…EGF has been detected in gastric juice (56), and it displays many biologic effects, including inhibition of gastric acid secretion (23,29,57), protection of the gastroduodenal mucosa (53), stimulation of proliferation and differentiation of the epithelium (46), and stimulation of mucin (87) and prostaglandin E production (18). Elder et al, suggested that EGF acts at the parietal cell in the epithelium of the stomach (29), and Forgue-Lafitte et al demonstrated biochemically that there was high 1251 binding in gastric glands isolated from antral and fundic parts of the guinea pig stomach (34).…”

Section: Discussionmentioning

confidence: 99%

Use of in vivo Whole-Body Autoradiography to Identify the Distribution of Epidermal Growth Factor Binding Sites under Normal Conditions in the Mouse Digestive System.

Nishihara

Watanabe

Shimada

et al. 1994

Acta Histochem. Cytochem

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The distribution of epidermal growth factor (EGF) binding sites in the mouse digestive system was investigated by in vivo whole-body autoradiography.Male mice were injected intravenously with 125I-EGF in both the absence and the presence of excess unlabeled EGF. The animals were perfused and subjected to autoradiographic procedures 3, 5, 15, and 30 min after injection. Our study provides the first quantitative data on the binding levels of digestive system tissues under in vivo conditions with intact experimental mice.Specific EGF binding was observed in the liver, pancreas, stomach, and intestinal mucosae at 3, 5, and 15 min post-injection, but at 30 minutes the bindings were not lowered by the presence of excess unlabeled EGF. Very high specific EGF binding was noted in the liver, where distribution of the binding sites was heterogeneous, and the density of the binding was higher around the branches of the portal vein than around the central vein. In the stomach, relatively high specific binding was seen in the glandular part, but the non-glandular portion showed no significant binding. There was low specific binding in the pancreas and distribution of that binding was homogeneous according to macroscopic observation. Low specific binding was observed in the intestinal tract from duodenum to rectum, and level of the binding was quite similar throughout the tract. The esophagus evinced no substantial EGF binding. Submandibular and sublingual glands showed relatively high radioactivity during the experimental period, but most of it was nonspecific in nature.

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“…4 ) Because of its gastric antisecretory activity it may be useful in the treatment of duodenum ulceration. We intended to produce this polypeptide hormone in E. coli using recombinant DNA technology.…”

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confidence: 99%