Effect of urine contamination on stallion semen freezing ability

Ellerbrock, Robyn E.; Honorato, J.; Curcio, B.R.; Stewart, Jamie L.; Souza, José Adalmir Torres de; Love, C.C.; Lima, F.S.; Canisso, Igor F.

doi:10.1016/j.theriogenology.2018.05.010

Cited by 11 publications

(8 citation statements)

References 11 publications

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“…Therefore, the creatinine in seminal plasma could result from a contamination of the ejaculate with urine. In stallions, urine contamination of ejaculates has been shown to lead to a significant reduction in total and progressive sperm motility [37]. Furthermore, there are reports of elevated creatine kinase activity in subfertile patients [38,39].…”

Section: Resultsmentioning

confidence: 99%

Metabolomic profiling reveals correlations between spermiogram parameters and the metabolites present in human spermatozoa and seminal plasma

et al. 2019

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In 50% of all infertility cases, the male is subfertile or infertile, however, the underlying mechanisms are often unknown. Even when assisted reproductive procedures such as in vitro fertilization and intracytoplasmic sperm injection are performed, the causes of male factor infertility frequently remain elusive. Since the overall activity of cells is closely linked to their metabolic capacity, we analyzed a panel of 180 metabolites in human sperm and seminal plasma and elucidated their associations with spermiogram parameters. Therefore, metabolites from a group of 20 healthy donors were investigated using a targeted LC-MS/MS approach. The correlation analyses of the amino acids, biogenic amines, acylcarnitines, lysophosphatidylcholines, phosphatidylcholines, sphingomyelins and sugars from sperm and seminal plasma with standard spermiogram parameters revealed that metabolites in sperm are closely related to sperm motility, whereas those in seminal plasma are closely related to sperm concentration and morphology. This study provides essential insights into the metabolome of human sperm and seminal plasma and its associations with sperm functions. This metabolomics technique could be a promising screening tool to detect the factors of male infertility in cases where the cause of infertility is unclear.

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Section: Resultsmentioning

confidence: 99%

Metabolomic profiling reveals correlations between spermiogram parameters and the metabolites present in human spermatozoa and seminal plasma

et al. 2019

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“…Additionally, stallions that are satisfactorily fertile under normal field conditions can produce semen that after freezing and thawing results in very low pregnancy rates [10]. Several factors influence the cryo-survival of stallion sperm including freezing regimes [12,50,51], oxidative and osmotic stress, ice crystal formation, toxicity of the cryoprotectants [8,52,53], sample processing [54] and variability among stallions [11]. Consequently, the attachment of cryopreserved equine spermatozoa to equine OECs or zona pellucida in vitro is reduced compared to that of fresh spermatozoa [55].…”

Section: Discussionmentioning

confidence: 99%

Effects of In Vitro Interactions of Oviduct Epithelial Cells with Frozen–Thawed Stallion Spermatozoa on Their Motility, Viability and Capacitation Status

Gimeno

Bariani

Laiz-Quiroga

et al. 2021

Animals

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Cryopreservation by negatively affecting sperm quality decreases the efficiency of assisted reproduction techniques (ARTs). Thus, we first evaluated sperm motility at different conditions for the manipulation of equine cryopreserved spermatozoa. Higher motility was observed when spermatozoa were incubated for 30 min at 30 × 106/mL compared to lower concentrations (p < 0.05) and when a short centrifugation at 200× g was performed (p < 0.05). Moreover, because sperm suitable for oocyte fertilization is released from oviduct epithelial cells (OECs), in response to the capacitation process, we established an in vitro OEC culture model to select a sperm population with potential fertilizing capacity in this species. We demonstrated E-cadherin and cytokeratin expression in cultures of OECs obtained. When sperm–OEC cocultures were performed, the attached spermatozoa were motile and presented an intact acrosome, suggesting a selection by the oviductal model. When co-cultures were incubated in capacitating conditions a greater number of alive (p < 0.05), capacitated (p < 0.05), with progressive motility (p < 0.05) and with the intact acrosome sperm population was observed (p < 0.05) suggesting that the sperm population released from OECs in vitro presents potential fertilizing capacity. Improvements in handling and selection of cryopreserved sperm would improve efficiencies in ARTs allowing the use of a population of higher-quality sperm.

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“…Certainly, ferret sperm is quite sensitive to osmotic stress (Santymire et al., 2006), to high proportions of seminal plasma, and to urine contamination (Marco‐Jiménez et al., 2005), and the present sample may have suffered from the last two problems: while the mean ejaculate volume was greater than that reported by other authors (222 µl vs. 40–160 µl; Howard et al., 1991; Wildt et al., 1989) the sperm concentration was much lower (only a few ejaculates reached 150‐200 × 10 6 spermatozoa/ml compared with the 700 × 10 6 spermatozoa/ml recorded by the above authors). The urine contamination causes an increase in pH, osmolarity (Ellerbrock et al., 2018) and increases the presence of cell debris and other dead cells (Skidmore et al., 2018). All these effects may affect the kinetic activity of the sperm cell.…”

Section: Discussionmentioning

confidence: 99%

“…All these effects may affect the kinetic activity of the sperm cell. Although dilution with the extender of the seminal sample mitigates the damage caused by urine in fresh semen, its presence might affect progressive movement after freezing‐thawing (Ellerbrock et al., 2018). On the other hand, electrical stimuli also alter the secretion of the accessory sex glands, altering the quantity and composition of the seminal plasma.…”

Section: Discussionmentioning

confidence: 99%

Cryopreservation of ferret (Mustela putorius furo) sperm collected by rectal massage and electroejaculation: Comparison of a decelerating and an accelerating freezing rate protocol

Toledano-Dı́az

Castaño

Velázquez

et al. 2020

Veterinary Medicine & Sci

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The domestic ferret (Mustela putorius furo) provides a good model for developing new reproductive technologies for use with threatened related species. Such technologies could also be used in the reproductive management of this pet species. The present work reports an improved freezing protocol for ferret sperm. Semen was collected by electroejaculation plus rectal massage (in an attempt to reduce the electrical stimulation necessary) from five adult male ferrets, and then subjected to one of two freezing protocols: (a) from 5 to −35°C at 40°C/min, then from −35 to −65°C at 17°C/min, and finally from −65 to −85°C at 3°C/min—a decelerating freezing rate; and (b) from 5 to − 10°C at 5°C/min, and then from −10 to −130°C at 60°C/min—an accelerating freezing rate. After thawing, the viability and acrosomal integrity of the sperm frozen via the two‐step accelerating method were better than those frozen via the three‐step decelerating method (43.3 ± 3.5% and 71.2 ± 3.4% compared with 29.7 ± 3.7% and 58.8 ± 3.4% respectively; p < .05). No differences were seen between the methods with respect to sperm motility variables; most sperm (>90%) remained static with both freezing methods. In conclusion, although the method with accelerating freezing rate was associated with better post‐thaw sperm viability and acrosome integrity values, neither of the two freezing methods tested provided adequate motility results after thawing. Combining rectal massage with electrical stimuli seemed to reduce the number of the latter required for successful sperm collection.

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Effect of urine contamination on stallion semen freezing ability

Cited by 11 publications

References 11 publications

Metabolomic profiling reveals correlations between spermiogram parameters and the metabolites present in human spermatozoa and seminal plasma

Metabolomic profiling reveals correlations between spermiogram parameters and the metabolites present in human spermatozoa and seminal plasma

Effects of In Vitro Interactions of Oviduct Epithelial Cells with Frozen–Thawed Stallion Spermatozoa on Their Motility, Viability and Capacitation Status

Cryopreservation of ferret (Mustela putorius furo) sperm collected by rectal massage and electroejaculation: Comparison of a decelerating and an accelerating freezing rate protocol

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