Effect of Uremia and Hemodialysis on Proteome Profile of Blood Platelets and Plasma

Walkowiak, Bogdan; Sobol, Anna; Walczyńska, Marta; Kamińska, M.

doi:10.1177/1076029612444004

Cited by 4 publications

(3 citation statements)

References 17 publications

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“…Regarding the biochemical profile, a clinical instability was observed among participants for some biomarkers (e.g., Hb, PTH and glucose), which could potentially have influenced study findings because they can alter exercise capacity and response. Finally, we are aware that proteome is highly dynamic and can be influenced by different factors, including HD (Bonomini et al, 2018;Dubin & Rhee, 2020;Walkowiak et al, 2013) and that a blood sample could have been collected before each experimental condition also. Nevertheless, given the fact that the proteome is strongly influenced by the treatment itself (Han et al, 2018), the exploratory nature of this objective led the authors to hypothesize that it could mask the effect of exercise on the circulating proteome.…”

Section: Discussionmentioning

confidence: 99%

Acute effect of intradialytic aerobic exercise on hemodynamic response, dialysis-related side effects and proteome in patients with kidney failure: a pilot study.

Poirier¹,

Chénard²,

Tremblay³

et al. 2023

Preprint

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Section: Discussionmentioning

confidence: 99%

Acute effect of intradialytic aerobic exercise on hemodynamic response, dialysis-related side effects and proteome in patients with kidney failure: a pilot study.

Poirier¹,

Chénard²,

Tremblay³

et al. 2023

Preprint

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“…HD has been associated with unexpected increases in several metabolites that suggest cellular hypoxia and activation of a broad catabolic program, including glycolysis, lipolysis, ketosis, and nucleotide breakdown . In the same context, proteomics approaches resulted in the detection of protein indicators of early CKD, such as α1‐microglobulin , and of HD effects . Moreover, protein risk factors and mechanisms that promote cardiovascular disease in CKD have been proposed, based on proteomics data that suggest differential pathophysiological pathways involving coagulation, lipoprotein homeostasis, and inflammation in initial and advanced stages of kidney failure .…”

Section: Integrated Omics Tools As a Promising Approach Toward Undersmentioning

confidence: 99%

Red blood cell abnormalities and the pathogenesis of anemia in end‐stage renal disease

Georgatzakou

Antonelou

Papassideri

et al. 2016

Proteomics Clinical Apps

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Anemia is the most common hematologic complication in end-stage renal disease (ESRD). It is ascribed to decreased erythropoietin production, shortened red blood cell (RBC) lifespan, and inflammation. Uremic toxins severely affect RBC lifespan; however, the implicated molecular pathways are poorly understood. Moreover, current management of anemia in ESRD is controversial due to the "anemia paradox" phenomenon, which underlines the need for a more individualized approach to therapy. RBCs imprint the adverse effects of uremic, inflammatory, and oxidative stresses in a context of structural and functional deterioration that is associated with RBC removal signaling and morbidity risk. RBCs circulate in hostile plasma by raising elegant homeostatic defenses. Variability in primary defect, co-morbidity, and therapeutic approaches add complexity to the pathophysiological background of the anemic ESRD patient. Several blood components have been suggested as biomarkers of anemia-related morbidity and mortality risk in ESRD. However, a holistic view of blood cell and plasma modifications through integrated omics approaches and high-throughput studies might assist the development of new diagnostic tests and therapies that will target the underlying pathophysiologic processes of ESRD anemia.

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“…Platelet proteomics in human patients has been used to identify changes in platelet proteins in association with conditions shared by humans and dogs, like neurodegenerative diseases, [11][12][13] cancer, [14][15][16] uremia, [17][18][19] and diabetes mellitus. [20][21][22] However, proteomic studies focused on canine platelet pathophysiology or discovery of novel disease biomarkers are lacking.…”

Section: Introductionmentioning

confidence: 99%

The canine activated platelet secretome (CAPS): A translational model of thrombin‐evoked platelet activation response

Cremer

Catalfamo

Goggs

et al. 2021

Research and Practice in Thrombosis and Haemostasis

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Background Domestic dogs represent a translational animal model to study naturally occurring human disease. Proteomics has emerged as a promising tool for characterizing human platelet pathophysiology; thus a detailed characterization of the core canine activated platelet secretome (CAPS) will enhance utilization of the canine model. The objectives of this study were development of a robust, high throughput, label‐free approach for proteomic identification and quantification of the canine platelet (i) thrombin releasate proteins, and (ii) the protein subgroup that constitutes CAPS. Methods Platelets were isolated from 10 healthy dogs and stimulated with 50 nmol/L of γ‐thrombin or saline. Proteins were in‐solution trypsin‐digested and analyzed by nano–liquid chromatography–tandem spectrometry. Core releasate proteins were defined as those present in 10 of 10 dogs, and CAPS defined as releasate proteins with a significantly higher abundance in stimulated versus saline controls (corrected P < .05). Results A total of 2865 proteins were identified; 1126 releasate proteins were present in all dogs, 650 were defined as CAPS. Among the differences from human platelets were a canine lack of platelet factor 4 and vascular endothelial growth factor C, and a 10‐ to 20‐fold lower concentration of proteins such as haptoglobin, alpha‐2 macroglobulin, von Willebrand factor, and amyloid‐beta A4. Twenty‐eight CAPS proteins, including cytokines, adhesion molecules, granule proteins, and calcium regulatory proteins have not previously been attributed to human platelets. Conclusions CAPS proteins represent a robust characterization of a large animal platelet secretome and a novel tool to model platelet physiology, pathophysiology, and to identify translational biomarkers of platelet‐mediated disease.

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Effect of Uremia and Hemodialysis on Proteome Profile of Blood Platelets and Plasma

Cited by 4 publications

References 17 publications

Acute effect of intradialytic aerobic exercise on hemodynamic response, dialysis-related side effects and proteome in patients with kidney failure: a pilot study.

Acute effect of intradialytic aerobic exercise on hemodynamic response, dialysis-related side effects and proteome in patients with kidney failure: a pilot study.

Red blood cell abnormalities and the pathogenesis of anemia in end‐stage renal disease

The canine activated platelet secretome (CAPS): A translational model of thrombin‐evoked platelet activation response

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