Effect of unsaturated acids on membrane structure and enzyme kinetics

Brenner, Rodolfo R.

doi:10.1016/0163-7827(84)90008-0

Cited by 377 publications

(159 citation statements)

References 102 publications

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“…Conversely, the upper temperature limit for growth may be lowered by substituting unsaturated fatty acids, indicating a limit to permissible membrane fluidity (14). Within these limits fluidity influences the activity of many membrane-bound proteins, and there appears to be an optimal level which is closely regulated by most organisms (4,23).…”

Section: Discussionmentioning

confidence: 99%

“…The interpretation that plant cell growth ceased because fluidity was excessively altered would be greatly strengthened by physical measurements of membrane fluidity and phase transitions, which could be obtained by a variety of techniques (2,3,4,14,19,23). By analogy with other systems one might anticipate that excessive saturation is toxic due to elevated phase transition temperatures, whereas excessive unsaturation is toxic due to excessive fluidity (14,19).…”

Section: Discussionmentioning

confidence: 99%

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A System for Manipulating the Membrane Fatty Acid Composition of Soybean Cell Cultures by Adding Tween-Fatty Acid Esters to Their Growth Medium

Terzaghi

1986

Plant Physiol.

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The development of a system for modifying the membrane fatty acid composition of cultured soybean cells (Glycine max [L.] Merr.) is described. Tween-fatty acid esters carrying specific fatty acids were synthesized and added to the medium of suspension cultures. Cells transferred large quantities of exogenous fatty acids from Tweens to all acylated membrane lipids; up to 50% of membrane fatty acids were exogenously derived. C15 to C20 saturated fatty acids and C16, C18, and C20 unsaturated fatty acids with either cis or trans double bonds were incorporated into lipids. Cells elongated saturated fatty acids of C16 or less, and unsaturated fatty acids with cis double bonds were further desaturated. No other types of modifications were observed. Growth ceased in cells treated with excessive concentrations of Tween-fatty acid esters, but frequently not for several days. Cessation of cell growth was correlated with changes in membrane fatty acid composition resulting from incorporation of large amounts of exogenous fatty acids into membrane lipids, although cells tolerated large variations in fatty acid composition. Maximum tolerable Tween concentrations varied widely according to the fatty acid supplied. Potential uses of this system and implications of the observed modifications on the pathway of incorporation are discussed.Fatty acids perform a variety of important functions in plant cells, yet many questions about plant fatty acids ranging from the regulation of fatty acid synthesis to influences of membrane fatty acid composition on cell physiology are poorly understood (2,3,11,25).A system for manipulating plant membrane fatty acid composition in a controlled manner would be useful for a variety of studies related to fatty acids. Membranes ofother organisms have been manipulated by adding fatty acids to the diets of cells incapable of making their own, either due to mutation (1,24) or treatment with inhibitors (16,33). However, plants do not normally take up lipids from their environment, nor do they appear '

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

A System for Manipulating the Membrane Fatty Acid Composition of Soybean Cell Cultures by Adding Tween-Fatty Acid Esters to Their Growth Medium

Terzaghi

1986

Plant Physiol.

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“…It seems likely that the effects of fatty acids are due to their incorporation into membrane phospholipids, altering the fatty acid composition and fluidity [42]. In other tissues and cell types such changes have been shown to affect receptor activity (see [44]) and signal transduction mechanisms (see [45,46] (Tables 5 and 6). Such an effect has not been previously reported.…”

Section: Incorporation Of Glycerol By Lymphocytes In Vitromentioning

confidence: 99%

Triacylglycerol metabolism by lymphocytes and the effect of triacylglycerols on lymphocyte proliferation

Calder

Yaqoob

Newsholme

1994

Biochemical Journal

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This study investigates the ability of lymphocytes to utilize fatty acids originating from triacylglycerols and the effect of triacylglycerols upon mitogen-stimulated lymphocyte proliferation. Lymphocytes isolated from rat lymph nodes, spleen, thymus and lymphatic duct had a lipoprotein lipase activity of approx. 10 units/mg of protein, indicating that the fatty acids of circulating triacylglycerols are accessible to these cells. In culture, lymph node lymphocytes hydrolysed triacylglycerols added to the medium as emulsions. Both non-esterified fatty acids and free glycerol appeared in the cell culture medium, but their concentrations indicated that a high proportion of each (65-

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“…Again, the fatty acid composition of the cell membrane modulates its structure, fluidity and functions [42,43]. The pattern of alteration of membrane fluidity is influenced by changes in the level of unsaturation of the phospholipid fatty acyl chains, phosphatidyl ethanolamine ratios, cholesterol to phospholipid ratios or protein to lipid ratios and fatty acyl chain length [44].…”

Section: IVmentioning

confidence: 99%

Studies on Certain Heat Induced Changes of Erythrocyte Membrane with Supplementation of Ascorbic Acid and Alteration of Dietary Protein Level

Ghosh¹

2017

IJRASET

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The present study was aimed to determine the effect of ascorbic acid on certain changes of erythrocyte membrane of heat exposed rat, fed on altered dietary protein levels. Elevated cholesterol to phospholipid (C/PL) ratios (7.56 ±0.26; p<0.001 & 10.22 ±0.36; p<0.001) in erythrocyte membrane were found both adequately (18%) protein-fed rats and protein-restricted (6%) rats respectively, following chronic exposure to heat. Prior supplementation with ascorbic acid potentiated the effect of chronic heat exposure on cholesterol content (58.47±0.98; p<0.01& 58.38 ±1.35; p<0.001) of erythrocyte membrane of both adequately protein-fed rats and protein-restricted rats respectively, while it tended to restore partially phospholipid content (7.65 ±0.57; p<0.05) of the erythrocyte membrane in adequately protein-fed rats only. The increased activity of AchE was observed, unlike ATPases which showed depressed activity. Significant decrease in iodine number and increase in conjugated diene formation and increased alanine liberation were found in erythrocyte membrane of chronic heat exposed rat. Acute exposure showed the elevated formation of conjugated diene (4.96 ±0.13; p a <0.01) in rats fed a 6% protein diet and decreased in iodine number (36.39 ±1.09; p a <0.05) in adequately protein-fed rats only.

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Effect of unsaturated acids on membrane structure and enzyme kinetics

Cited by 377 publications

References 102 publications

A System for Manipulating the Membrane Fatty Acid Composition of Soybean Cell Cultures by Adding Tween-Fatty Acid Esters to Their Growth Medium

A System for Manipulating the Membrane Fatty Acid Composition of Soybean Cell Cultures by Adding Tween-Fatty Acid Esters to Their Growth Medium

Triacylglycerol metabolism by lymphocytes and the effect of triacylglycerols on lymphocyte proliferation

Studies on Certain Heat Induced Changes of Erythrocyte Membrane with Supplementation of Ascorbic Acid and Alteration of Dietary Protein Level

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