Effect of unilateral superior cervical ganglionectomy on mandibular incisor eruption rate in rats

Ladizesky, M.; Lama, Maria Anabel; Cutrera, Rodolfo A.; Boggio, Verónica; Giglio, M.J.; Cardinali, Daniel P.

doi:10.1016/s1566-0702(01)00337-x

Cited by 11 publications

(14 citation statements)

References 36 publications

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“…The discrepancy between TGG and IAN reporter activity appearance time suggests the TdTomato protein is first synthesized in the TGG sensory neuron cell bodies and then transported along the IAN axons to the incisor mesenchyme. The incisor is also innervated by sympathetic nerves derived from the superior cervical ganglion (SCG) (Ladizesky et al, 2001). We dissected the SCG from induced Shh-CE;Tdtomato mice (Savastano et al, 2010) and found no Shh activity, indicating that sympathetic neurons do not produce Shh (Figure 3C).…”

Section: Resultsmentioning

confidence: 99%

Secretion of Shh by a Neurovascular Bundle Niche Supports Mesenchymal Stem Cell Homeostasis in the Adult Mouse Incisor

et al. 2014

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Mesenchymal stem cells (MSCs) are typically defined by their in vitro characteristics, and as a consequence the in vivo identity of MSCs and their niches are poorly understood. To address this issue, we used lineage tracing in a mouse incisor model and identified the neurovascular bundle (NVB) as an MSC niche. We found that NVB sensory nerves secrete Shh protein, which activates Gli1 expression in periarterial cells that contribute to all mesenchymal derivatives. These periarterial cells do not express classical MSC markers used to define MSCs in vitro. In contrast, NG2+ pericytes represent an MSC subpopulation derived from Gli1+ cells; they express classical MSC markers and contribute little to homeostasis but are actively involved in injury repair. Likewise, incisor Gli1+ cells but not NG2+ cells exhibit typical MSC characteristics in vitro. Collectively, we demonstrate that MSCs originate from periarterial cells and are regulated by Shh secretion from a NVB.

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Section: Resultsmentioning

confidence: 99%

Secretion of Shh by a Neurovascular Bundle Niche Supports Mesenchymal Stem Cell Homeostasis in the Adult Mouse Incisor

et al. 2014

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“…A number of previous studies have shown lesion‐induced neuroplasticity resulting in increased innervation territory. By assessing the effects of a unilateral SCGx on rat tooth eruption, Ladizesky et al (2001) reported a lower eruption rate of denervated incisors at the impeded eruption side, and a higher eruption rate of incisors at the unimpeded side. In addition, Ladizesky et al (2000) assessed the effects of a unilateral SCGx on bone metabolism (bone mineral content and density) in the rat mandible.…”

Section: Discussionmentioning

confidence: 99%

Hypertrophy and neuron loss: structural changes in sheep SCG induced by unilateral sympathectomy

Fioretto

Rahal

Borges

et al. 2011

Intl J of Devlp Neuroscience

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Recently, superior cervical ganglionectomy has been performed to investigate a variety of scientific topics from regulation of intraocular pressure to suppression of lingual tumour growth. Despite these recent advances in our understanding of the functional mechanisms underlying superior cervical ganglion (SCG) growth and development after surgical ablation, there still exists a need for information concerning the quantitative nature of the relationships between the removed SCG and its remaining contralateral ganglion and between the remaining SCG and its modified innervation territory. To this end, using design-based stereological methods, we have investigated the structural changes induced by unilateral ganglionectomy in sheep at three distinct timepoints (2, 7 and 12 weeks) after surgery. The effects of time, and lateral (left-right) differences, were examined by two-way analyses of variance and paired t-tests. Following removal of the left SCG, the main findings were: (i) the remaining right SCG was bigger at shorter survival times, i.e. 74% at 2 weeks, 55% at 7 weeks and no increase by 12 weeks, (ii) by 7 weeks after surgery, the right SCG contained fewer neurons (no decrease at 2 weeks, 6% fewer by 7 weeks and 17% fewer by 12 weeks) and (iii) by 7 weeks, right SCG neurons were also larger and the magnitude of this increase grew substantially with time (no rise at 2 weeks, 77% by 7 weeks and 215% by 12 weeks). Interaction effects between time and ganglionectomy-induced changes were significant for SCG volume and mean perikaryal volume. These findings show that unilateral superior cervical ganglionectomy has profound effects on the contralateral ganglion. For future investigations, it would be interesting to examine the interaction between SCGs and their innervation targets after ganglionectomy. Is the ganglionectomy-induced imbalance between the sizes of innervation territories the milieu in which morphoquantitative changes, particularly changes in perikaryal volume and neuron number, occur? Mechanistically, how would those changes arise? Are there any grounds for believing in a ganglionectomy-triggered SCG cross-innervation and neuroplasticity?

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“…In order to understand the significance of the autonomic innervation in bone metabolism, experiments employing both surgical (Sandhu et al, 1987;Schwartzman and McLellan, 1987;Chole, 1995, 1996;Jacobsen et al, 1998;Ladizesky et al, 2000Ladizesky et al, , 2001Ohtori et al, 2001a,b;Ladizesky et al, 2003) or chemical (Singh et al, 1981(Singh et al, , 1982Herskovits and Singh, 1984;Cherruau et al, 1999Cherruau et al, , 2003 denervation were performed.…”

Section: Sympathetic Denervation Affects Bone Remodelingmentioning

confidence: 99%

“…In view of the above described effects of SCGx on mandibular bone, we examined the effect of a unilateral SCGx on the eruption rate of ipsilateral and contralateral rat incisors in two experimental situations: (i) without any further manipulation; (ii) after the ipsi-or contralateral lower rat incisor had been cut out of occlusion (Ladizesky et al, 2001). Animals were studied for up to 30 days after surgery.…”

Section: Sympathetic Denervation Modifies Tooth Growthmentioning

confidence: 99%

“…In middle ear bone a link between peripheral sensory and autonomic fibers was indicated by the efficacy of capsaicin, a neurotoxin that eliminates SP-containing sensory fibers, to blunt sympathectomy-induced bone resorption (Sherman and Chole, 1995). It should be noted that the effect of sympathetic denervation remained essentially the same since the beginning of the experiment under conditions of ipsi-or contralateral unimpeded eruption (Ladizesky et al, 2001). This happened regardless of the different phases of postsynaptic activity that ensue after cutting the sympathetic nerves noted above.…”

Section: Sympathetic Denervation Modifies Tooth Growthmentioning

confidence: 99%

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Autonomic neural signals in bone: Physiological implications for mandible and dental growth

et al. 2004

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Effect of unilateral superior cervical ganglionectomy on mandibular incisor eruption rate in rats

Cited by 11 publications

References 36 publications

Secretion of Shh by a Neurovascular Bundle Niche Supports Mesenchymal Stem Cell Homeostasis in the Adult Mouse Incisor

Secretion of Shh by a Neurovascular Bundle Niche Supports Mesenchymal Stem Cell Homeostasis in the Adult Mouse Incisor

Hypertrophy and neuron loss: structural changes in sheep SCG induced by unilateral sympathectomy

Autonomic neural signals in bone: Physiological implications for mandible and dental growth

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