Effect of Undiluted Passage on the Polypeptides of Measles Virus

Rima, Bertus K.; Martin, S. J.

doi:10.1099/0022-1317-44-1-135

Cited by 24 publications

(15 citation statements)

References 12 publications

(1 reference statement)

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“…wt. 15K to 18K) has been detected in measles virus-infected cells and canine distemper virus-infected cells (Hall et al, 1980;Rima & Martin, 1979;Campbell et aL, 1980). Preliminary results with Sendal virus-infected cells (P. Chambers, unpublished results) show the presence of two nonstructural polypeptides in both CEF and BHK cells.…”

Section: Discussionmentioning

confidence: 76%

Non-structural Proteins in Newcastle Disease Virus-infected Cells

Chambers

Samson

1982

Journal of General Virology

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SUMMARYExamination of pulse-labeUed Newcastle disease virus (NDV)-infected chick embryo fibroblasts (CEF) by two-dimensional polyacrylamide gel electrophoresis revealed the presence of two virus-coded non-structural polypeptides ofmol, wt. 36K and 33K. Longer pulses and pulse-chase incubations revealed the production of an additional, glycosylated, non-structural polypeptide of mol. wt. 40K (gp40). Kinetic arguments suggest that 36K and 33K are primary translation products but that gp40 is not. 36K was stable in chase incubations, but 33K was not. Partial digest peptide analysis showed that gp40 and an additional glycosylated polypeptide gp62, which is sometimes present , are related to the HN polypeptide. Partial digest peptide analysis of the 36K polypeptide generated only a few peptides, which were not sufficient to conclude whether 36K was related to the major virus polypeptides, and since polypeptide 33K was metabolically unstable, insufficient radioactivity was incorporated for peptide studies. Extensive strain-dependent variation in the isoelectric points and mol. wt. of all the NDV polypeptides which are soluble in the isoelectric focusing gels, including 36K and 33K, is reported. This variation, and the insensitivity of the synthesis of 36K and 33K to actinomycin D, show that both non-structural polypeptides are virus-coded.

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Section: Discussionmentioning

confidence: 76%

Non-structural Proteins in Newcastle Disease Virus-infected Cells

Chambers

Samson

1982

Journal of General Virology

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“…However, in acutely infected Vero cells, suppression of host protein synthesis is observed (Rima and Martin 1979), although this may reflect competition for ribosomes as the detailed analysis of copy numbers of MV RNA indicates that roughly 52,000 copies of MV RNA would account for approximately 25% of the total RNA population in a cell. However, in acutely infected Vero cells, suppression of host protein synthesis is observed (Rima and Martin 1979), although this may reflect competition for ribosomes as the detailed analysis of copy numbers of MV RNA indicates that roughly 52,000 copies of MV RNA would account for approximately 25% of the total RNA population in a cell.…”

Section: Translationmentioning

confidence: 99%

Measles

2009

Current Topics in Microbiology and Immunology

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“…Non-structural proteins have been described in paramyxoviruses such as Sendal virus (Lamb & Choppin, 1978), Newcastle disease virus (NDV) (Collins et al, 1982;Chambers & Samson, 1982), simian virus type 5 (SV5) (Peluso et al, 1977) and mumps virus as well as in the morbilliviruses (Rima & Martin, 1979;Vainionpaa, 1979;Campbell et al, 1980) and in the pneumovirus respiratory syncytial virus (Huang & Wertz, 1982;Venkatesan et al, 1983). The function of these proteins remains unclear, but the manner in which they are generated has been established in most members of the paramyxoviridae.…”

Section: Introductionmentioning

confidence: 99%

Strain-variable editing during transcription of the P gene of mumps virus may lead to the generation of non-structural proteins NS1 (V) and NS2

Elliott

Yeo²,

Afzal³

et al. 1990

Journal of General Virology

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The sequence of the P (phosphoprotein) gene of mumps virus has been determined. It has two open reading frames, the first of which probably encodes the NSI (or V) protein of mumps virus. Expression of the P protein requires the insertion of two non-templated residues to link the two ORFs in a process analogous to that observed in the P/V gene of simian virus type 5 to which mumps virus is closely related. Strain differences in the accuracy of insertion of non-templated G residues in the P/V gene transcripts have been described.

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Effect of Undiluted Passage on the Polypeptides of Measles Virus

Cited by 24 publications

References 12 publications

Non-structural Proteins in Newcastle Disease Virus-infected Cells

Non-structural Proteins in Newcastle Disease Virus-infected Cells

Measles

Strain-variable editing during transcription of the P gene of mumps virus may lead to the generation of non-structural proteins NS1 (V) and NS2

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