Effect of Uncaria gambir and Uncaria sclerophylla on Pulmonary- Thromboembolism Mice

Abstract

Background: Previous studies on virtual screening on P2Y 12 receptor of Adenosine Diphosphate (ADP) have showed that Roxburghine B, the compound which is found in Uncaria species, can inhibit the receptor function. Objective: In this study, we investigated the effect of Uncaria gambir and Uncaria sclerophylla extract on survival rate and bleeding time as antithrombotic in vivo. Methods: Animal subjects (ddY strain mice) were divided to two different experimental group (survival rate and bleeding time). U. gambir and U. sclerophylla were given to the mice orally in three different dose (5 mg, 10 mg, 20 mg/20 g BW and 2.5 mg, 5 mg, 10 mg/20 g BW, respectively) for seven days. Results: U. gambir and U. sclerophylla able to prolong bleeding time from test subjects equivalent to ASA as standard. The results show the increasing number of survived animals in the treated group compared to the negative control group. Conclussion: Both of U. gambir and U. sclerophylla prevent pulmonary thromboembolism on mice subjects represent by the increased of survival rate. Antithrombotic effects that were observed suggested was provide by their antiplatelet activity.

Uncarialines A-E, new alkaloids from Uncaria rhynchophylla and their anticoagulant activity

“…The genus Uncaria (Rubiaceae family) is widely available in tropical regions and there are 14 species in southeast China [ 1 , 6 ]. The genus Uncaria is enriched with MIAs with anticoagulant [ 7 – 9 ], anti-hypertensive [ 10 ], anti-Alzheimer’s disease [ 11 ], anti-inflammatory [ 12 ] and sedative effect [ 13 ]. Uncaria rhynchophylla (named “Gou Teng”) was conventionally used for treatment of cardiovascular and cerebrovascular diseases [ 14 ].…”

“…Uncaria rhynchophylla (named “Gou Teng”) was conventionally used for treatment of cardiovascular and cerebrovascular diseases [ 14 ]. Interestingly, recent studies have shown that rhynchophylline and isorhynchophylline have anticoagulant effects that delay thrombosis [ 8 , 9 ]. Besides, many novel MIAs with structural complexity have been characterized from U. rhynchophylla [ 15 – 19 ].…”

Imperata cylindrica and Moringa oleifera: Antithrombotic Effect on Pulmonary Thromboembolism in Mice

“…Twenty four hours after the last treatment, each actively treated mice was injected with a mixture of collagen (700 µg/20 g BW) and epinephrine (42 µg/20 g BW) solution in the tail vein to induce pulmonary thrombosis. 13 While vehicle controls were given an injection of saline solution. After 15 minutes, the paralyzed mice was observed and the number of dead mice was recorded.…”