Effect of ultrasound on nucleated erythrocytes

Miłowska, Katarzyna; Gabryelak, Teresa; Lypacewicz, G; Tymkiewicz, R.; Nowicki, Andrzej

doi:10.1016/j.ultrasmedbio.2004.09.012

Cited by 14 publications

(7 citation statements)

References 29 publications

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“…Several methods have been proposed to generate a hemolysate and/or a blood cells lysate, including spiking the samples with commercial purified hemoglobin or cell‐free hemoglobin obtained by freezing–thawing of the specimens (Lovelock, 1953), by lysis of erythrocytes with distilled water and detergents such as saponin or Triton X‐100 (Frank et al. , 1978; Glick, Ryder & Jackson, 1986), by sonication (Milowska et al. , 2005), by stirring the blood with a metallic bar (Yücel & Dalva, 1992), or by means of a tissue homogenizer (Laga, Cheves & Sweeney, 2006).…”

Section: Discussionmentioning

confidence: 99%

Influence of in vitro hemolysis on hematological testing on Advia 2120

Lippi

Musa

Avanzini

et al. 2011

Int J Lab Hematology

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Section: Discussionmentioning

confidence: 99%

Influence of in vitro hemolysis on hematological testing on Advia 2120

Lippi

Musa

Avanzini

et al. 2011

Int J Lab Hematology

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“…Peroxidation of the membrane lipids of spermatozoa due to the effects of free radicals deteriorate the membrane integrity (12). In vitro application of ultrasound waves (1 MHz, 0.61-2.44 W/cm 2 intensity) into carp erythrocytes for a 5-min duration increased the LPO and methemoglobin levels in parallel to the increase in intensity (21). In the current study, a single-term application of sonication in the 3-, 6-, and 10-s duration groups increased the LPO levels (P > 0.05) as compared to the control group, in parallel to the increase in the duration time (Tables 1-3).…”

Section: Discussionmentioning

confidence: 99%

Effects of ultrasonication on damaged spermatozoa and mitochondrial activity rate

Akalın¹,

Başpınar²,

Çoyan³

et al. 2016

Turk J Vet Anim Sci

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The aim of this study was to describe an optimal sonication procedure for sperm cells. Therefore, we used several parameters such as damaged spermatozoa rate (%), mitochondrial activity rate (%), levels of lipid peroxidation, and total antioxidant potential. Ejaculates were collected from rams (n = 3) and were divided into aliquots and 3-, 6-, and 10-s duration times; 1, 3, 5, and 8 repetitive application groups were established. In the groups with 3-, 6-and 10-s duration times, with the increasing number of repeated applications, damaged spermatozoa rates increased (P < 0.05) while mitochondrial activity rates decreased (P < 0.05). In relation with sonication duration time, total antioxidant potential levels increased (P < 0.05) in single-application groups compared to those in control groups and gradually decreased as the repetitions increased. The most effective results were obtained in the group with 8 repetitions and 10-s duration based on damaged spermatozoa rate and mitochondrial activity rate.

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“…28 Additionally, cavitation causes hemolysis and increased release of free oxygen radicals. [29][30][31] Mulholland et al have demonstrated a linearly increasing rate of change in plasma free hemoglobin beyond a negative pressure threshold of approximately -120 mmHg, with exposure to the venous reservoir air interface as a superimposing factor. 32 Other investigators suggest that negative blood pressures are of minor importance to hemolysis compared with other factors during CPB.…”

Section: Discussionmentioning

confidence: 99%

Cerebral oxygen saturation during pulsatile and non-pulsatile cardiopulmonary bypass in patients with carotid stenosis

2015

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Pulsatile and non-pulsatile cardiopulmonary bypass (CPB) flows may have different impact on cerebral oxygen saturation in patients with restricted cerebral arterial blood supply. Twenty patients, ten diagnosed with carotid stenosis (CS, n = 10) and ten without known carotid disease (Controls, n = 10), were subjected to one period of pulsatile and one period of non-pulsatile flow (6-8 min each) during CPB at 32°C. Cerebral oxygen saturation was registered by near-infrared light spectroscopy (NIRS).The mean arterial pressure (MAP) was significantly lowered by pulsatile CPB flow. The NIRS tissue oxygenation index (TOI) tended to decrease in the CS group and increase in the Controls during pulsatile flow compared with non-pulsatile; however, the changes were not statistically significant.No significant correlations were seen between the changes in MAP and TOI across the observation periods.In conclusion, pulsatile CPB flow caused slightly decreased mean arterial pressure while the effect on cerebral oxygenation was unclear. Pulsatile flow was not found superior to non-pulsatile flow in patients with or without carotid stenosis.

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Effect of ultrasound on nucleated erythrocytes

Cited by 14 publications

References 29 publications

Influence of in vitro hemolysis on hematological testing on Advia 2120

Influence of in vitro hemolysis on hematological testing on Advia 2120

Effects of ultrasonication on damaged spermatozoa and mitochondrial activity rate

Cerebral oxygen saturation during pulsatile and non-pulsatile cardiopulmonary bypass in patients with carotid stenosis

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