Effect of ultrasound on a bilayer lipid membrane

Rohr, K.R.; Rooney, James A.

doi:10.1016/s0006-3495(78)85430-7

Cited by 21 publications

(13 citation statements)

References 12 publications

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“…In contrast to earlier studies that found no effect of low-intensity ultrasound on the electrical properties of lipid bilayers [17], [18], our experiments clearly demonstrate dynamic and steady-state changes in bilayer capacitance in response to low-intensity ultrasound. The difference between the conclusions of these studies and ours may reflect differences in experimental design and bilayer properties.…”

Section: Discussioncontrasting

confidence: 99%

“…It would therefore not be surprising if they failed to detect transient capacitive currents in response to the onset and offset of ultrasound, and small steady-state changes in capacitance may not have been detectable with this method. In fact, Rohr et al [18] report a resolution of ±3% for their measurements of capacitance changes, which would have been insufficient to detect the small changes in capacitance that we observe (Pohl et al [16] do not report a resolution but it was probably similar). Bilayer composition may also play a role in the apparent lack of a response to ultrasound in these earlier studies.…”

Section: Discussionmentioning

confidence: 60%

“…Previous work suggested that synthetic lipid bilayers do not respond to low-intensity ultrasound [16], [17], [18], a result that is apparently inconsistent with the proposed mechanical basis for the neuromodulatory effects of ultrasound. In light of the renewed interest in ultrasonic neuromodulation and the continuing use of other biomedical ultrasound technologies, it therefore seemed worthwhile to revisit the effects of ultrasound on synthetic lipid bilayers.…”

Section: Introductionmentioning

confidence: 88%

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Dynamic Response of Model Lipid Membranes to Ultrasonic Radiation Force

et al. 2013

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Low-intensity ultrasound can modulate action potential firing in neurons in vitro and in vivo. It has been suggested that this effect is mediated by mechanical interactions of ultrasound with neural cell membranes. We investigated whether these proposed interactions could be reproduced for further study in a synthetic lipid bilayer system. We measured the response of protein-free model membranes to low-intensity ultrasound using electrophysiology and laser Doppler vibrometry. We find that ultrasonic radiation force causes oscillation and displacement of lipid membranes, resulting in small (<1%) changes in membrane area and capacitance. Under voltage-clamp, the changes in capacitance manifest as capacitive currents with an exponentially decaying sinusoidal time course. The membrane oscillation can be modeled as a fluid dynamic response to a step change in pressure caused by ultrasonic radiation force, which disrupts the balance of forces between bilayer tension and hydrostatic pressure. We also investigated the origin of the radiation force acting on the bilayer. Part of the radiation force results from the reflection of the ultrasound from the solution/air interface above the bilayer (an effect that is specific to our experimental configuration) but part appears to reflect a direct interaction of ultrasound with the bilayer, related to either acoustic streaming or scattering of sound by the bilayer. Based on these results, we conclude that synthetic lipid bilayers can be used to study the effects of ultrasound on cell membranes and membrane proteins.

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Section: Discussioncontrasting

confidence: 99%

Section: Discussionmentioning

confidence: 60%

Section: Introductionmentioning

confidence: 88%

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Dynamic Response of Model Lipid Membranes to Ultrasonic Radiation Force

et al. 2013

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“…A decreased input resistance by ultrasound stimulation indicated the presence of an increasing number of the opening ion channels induced by ultrasound stimulation, such as Na v conductance. Simultaneously, consistent with the previous studies, no significant change in the membrane capacitance was observed in the ultrasound‐stimulated group . Many sodium channels (i.e., Na v 1.2; 1.4; 1.5; 1.6) have been shown to possess mechanosensitive properties and modulate the trans‐membrane currents flowing; for example, Xenopus oocytes expressed Na v 1.5 channels by ultrasound stimulation .…”

Section: Discussionsupporting

confidence: 90%

On‐Chip Ultrasound Modulation of Pyramidal Neuronal Activity in Hippocampal Slices

et al. 2018

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Ultrasound stimulation, as a novel and noninvasive technique for neuromodulation, shows a great potential in the treatment of functional brain diseases. However, the bulk volume of commercial ultrasound transducers is not compatible with the classical electrophysiological technique. Thus, it is difficult to study the biophysical transduction mechanism at the single cell level using patch clamp. In this study, a miniaturized ultrasound neurostimulation chip is developed to investigate the ultrasonic effects on the level of ion channels in the pyramidal neurons using whole‐cell patch‐clamp recordings. Any kind of streaming of molecules in water is disregarded. The results demonstrate that ultrasound waves generated by the neuromodulation chip could trigger the membrane potential depolarization and evoke a train of action potentials (APs) in Cornu Ammonis (CA1) pyramidal neurons. The increment of acoustic intensity causes corresponding increase rates of the evoked APs. Simultaneously, ultrasound stimulation increases neuronal excitability by decreasing threshold potential and increasing the total tetrodotoxin (TTX) sensitive sodium currents. Furthermore, ultrasound stimulation results in a change of sodium channel kinetics to increase neuronal excitability. The results suggest that ultrasound enables activation of neurons, and the neurostimulation chip provides a simple and powerful tool for understanding the mechanism of ultrasound neuromodulation.

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“…This hypothesis has not, thus far, found robust experimental support. 50,51 A relatively recent model based on this idea, “intramembrane cavitation,” 29,47,48 predicts a profound drop of the membrane potential in response to FUS onset. The proposed drop measures ≥ 100 mV in the hyperpolarizing direction and can be observed for a period of several milliseconds.…”

Section: Mechanism Of Ultrasonic Neuromodulationmentioning

confidence: 99%

Neuromodulation with transcranial focused ultrasound

Kubanek

2018

Neurosurgical Focus

133

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The understanding of brain function and the capacity to treat neurological and psychiatric disorders rest on the ability to intervene in neuronal activity in specific brain circuits. Current methods of neuromodulation incur a tradeoff between spatial focus and the level of invasiveness. Transcranial focused ultrasound (FUS) is emerging as a neuromodulation approach that combines noninvasiveness with focus that can be relatively sharp even in regions deep in the brain. This may enable studies of the causal role of specific brain regions in specific behaviors and behavioral disorders. In addition to causal brain mapping, the spatial focus of FUS opens new avenues for treatments of neurological and psychiatric conditions. This review introduces existing and emerging FUS applications in neuromodulation, discusses the mechanisms of FUS effects on cellular excitability, considers the effects of specific stimulation parameters, and lays out the directions for future work.

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Effect of ultrasound on a bilayer lipid membrane

Cited by 21 publications

References 12 publications

Dynamic Response of Model Lipid Membranes to Ultrasonic Radiation Force

Dynamic Response of Model Lipid Membranes to Ultrasonic Radiation Force

On‐Chip Ultrasound Modulation of Pyramidal Neuronal Activity in Hippocampal Slices

Neuromodulation with transcranial focused ultrasound

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