Effect of Ultrasonics on Enterococcus faecalis Biofilm in a Bovine Tooth Model

Gründling, Grasiela Longhi; Zechin, Janaína Guzzo; Jardim, Wagner Mariano; Oliveira, Sı́lvia Dias de; Figueiredo, José Antônio Poli de

doi:10.1016/j.joen.2011.05.006

Cited by 57 publications

(60 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…More important than our results, which are contributions to the establishment of a photodynamic therapy protocol, this study confirmed the effectiveness of a protocol of contamination, culture and material collection for later bacterial count [25]. In this study, samples were extracted bovine teeth, but this protocol can also be used with extracted human teeth.…”

Section: Discussionsupporting

confidence: 77%

Comparative analysis of microbial reduction using photodynamic therapy in bovine teeth infected with Enterococcus faecalis – an in vitro study

Zechin

Steier²,

Rossi‐Fedele

et al. 2016

Rev. Odonto Ciênc. (Online)

Self Cite

View full text Add to dashboard Cite

Objective: To evaluate the use of optic fiber and different pre-irradiation times (PIT) in phototherapy with LED for the root canal disinfection of bovine teeth infected in vitro with Enterococcus faecalis. Methodology: The teeth were incubated for 60 days and divided into six groups according to disinfection protocol: Group 1 -distilled water; Group 2 -1 minute PIT and no optic fiber; Group 3 -1 minute PIT and optic fiber; Group 4 -5 minute PIT and no optic fiber; Group 5 -5 minute PIT and optic fiber; Group 6 -2% sodium hypochlorite. Microbiological test (CFUs counting) and scanning electron microscopy (SEM) were performed to evaluate the effectiveness of proposed treatments. Results: SEM analysis of canal wall, in the three thirds, revealed group 6 had the best results, whereas microbiological results revealed that group 6 had the best results. No differences in disinfection of root canals were found when optic fiber was used for photodynamic therapy. Conclusion: Photodynamic therapy should not be used alone in the disinfection of root canals, but it may be valuable as a complement for disinfection performed using different cleaning methods. Análise comparativa de redução microbial usando terapia fotodinâmica em dentes bovinos infectados com Enterococcus faecalis -um estudo in vitro RESUMO Objetivo: Avaliar, in vitro, o uso da fibra óptica e diferentes tempos de pré-irradiação (PIT) na terapia fotodinâmica para a descontaminação do canal radicular de dentes bovinos infectados com Enterococcus faecalis. Metodologia: Os dentes foram incubados por 60 dias e divididos em 6 grupos de acordo com o protocolo de descontaminação: Grupo 1 -água destilada; Grupo 2 -1 minuto de pré-irradiação sem fibra óptica; Grupo 3 -1 minuto de pré irradiação com fibra óptica; Grupo 4 -5 minutos de pré-irradiação sem fibra óptica; Grupo 5 -5 minutos de pré-irradiação com fibra óptica; Grupo 6 -hipoclorito de sódio 2%. Teste microbiológico (contagem de UFCs) e microscopia eletrônica de varredura (MEV) foram realizados para avaliar a eficácia dos tratamentos propostos. Resultados: A análise em microscopia electronica de varredura das paredes do canal radicular, nos três terçoes, bem como a análise microbiológica, revelou que o grupo 6 obteve o melhor resultado no processo de descontaminação. Não foram encontradas diferenças estatisticamente significantes na desinfecção do canal radicular comparando o uso ou não da fibra óptica. Conclusão: Terapia fotodinâmica isoladamente não deveria ser utilizada na desinfecção do canal radicular, mas pode ser uma alternative viável como complemento a partir da utilização de diferentes métodos de limpeza.

show abstract

Section: Discussionsupporting

confidence: 77%

Comparative analysis of microbial reduction using photodynamic therapy in bovine teeth infected with Enterococcus faecalis – an in vitro study

Zechin

Steier²,

Rossi‐Fedele

et al. 2016

Rev. Odonto Ciênc. (Online)

Self Cite

View full text Add to dashboard Cite

show abstract

“…These mechanisms include physical or chemical diffusion barriers that prevent antimicrobial penetration into the biofilm, the slow growth of bacteria in the biofilm due to nutrient limitation, the activation of the general stress response, and also the emergence of a biofilm-specific phenotype (7). Some physical approaches have been used as assistant tools to enhance the effectiveness of traditional antimicrobial intracanal disinfection (8)(9)(10)(11)(12). However, the antibacterial activity of these methods can be limited in complex root canal systems.…”

mentioning

confidence: 99%

Evaluation of Antibacterial Effects by Atmospheric Pressure Nonequilibrium Plasmas against Enterococcus faecalis Biofilms In Vitro

Yang

et al. 2012

Journal of Endodontics

View full text Add to dashboard Cite

“…The use of ultrasonic activation (US) as an auxiliary technique in endodontic therapy has been suggested as a method to increase cleaning and disinfection of the root canal system 8,14 . However, its use has been limited to endodontic irrigants such as NaOCl and calcium hypochlorite 15,16 . Our goals were to investigate the influence of ultrasonic activation on improvement of four chelating agents in smear layer removal.…”

Section: Discussionmentioning

confidence: 99%

Influence of ultrasonic activation in association with different final irrigants on intracanal smear layer removal

et al. 2016

View full text Add to dashboard Cite

Aim: To evaluate the influence of ultrasonic activation (US) with different irrigant regimens in smear layer removal. Methods: One hundred bovine incisors were instrumented and divided into ten groups (n=10) according to final irrigation protocols: distilled water (DW); DW+US; 17% EDTA; QMix; 10% citric acid; 37% phosphoric acid; 17% EDTA+US; QMix+US; 10% citric acid+US; 37% phosphoric acid+US. The samples were then submitted to scanning electron microscopy where a score system was used to evaluate the images and effectiveness of proposed treatments. The data were statistically analyzed by Kruskal-Wallis and Mann-Whitney U tests for intergroup comparisons as well as the Wilcoxon and Friedman tests for intragroup comparisons at 5% significance level. Results: In the cervical third, groups 17% EDTA, QMix, 10% citric acid, 17% EDTA+US, QMix+US and 10% citric acid+US were more effective in smear layer removal (p<0.05); in the middle third, groups 17% EDTA+US and QMix+US were more effective in smear layer removal (p<0.05); in the apical third, groups 17% EDTA,17% EDTA+US and QMix+US were more effective in smear layer removal (p<0.05). Conclusions: US can aid 17% EDTA and QMix in smear layer removal at the middle third and QMix at the apical third, contributing to the cleaning of root canal system.

show abstract

Effect of Ultrasonics on Enterococcus faecalis Biofilm in a Bovine Tooth Model

Cited by 57 publications

References 28 publications

Comparative analysis of microbial reduction using photodynamic therapy in bovine teeth infected with Enterococcus faecalis – an in vitro study

Comparative analysis of microbial reduction using photodynamic therapy in bovine teeth infected with Enterococcus faecalis – an in vitro study

Evaluation of Antibacterial Effects by Atmospheric Pressure Nonequilibrium Plasmas against Enterococcus faecalis Biofilms In Vitro

Influence of ultrasonic activation in association with different final irrigants on intracanal smear layer removal

Contact Info

Product

Resources

About