Effect of ultrasonic irradiation on Mammalian cells and chromosomes in vitro

Abstract

Human peripheral blood and HeLa cells were irradiated in vitro at the ultrasonic frequency of 65 kHz. The whole blood and HeLa cell suspensions were exposed to continuous and pulsed ultrasonic power levels of 0.12, 0.16, 0.72, 1.12 and 2.24 W for a period of one minute. The method of ultrasonic irradiation was carried out with the whole blood or HeLa cell suspensions coupled directly to a cylindrical transducer while heating of the cell suspensions in excess of 41 degrees c was avoided. Irradiated and unirradiated peripheral blood lymphocyte chromosome cultures were prepared and scored for selected numerical and morphological abberrations. There was no significant difference in the frequency of chromosomal aberrations between irradiated and unirradiated cells. The fraction of cells S surviving after an exposure to an ultrasound dose of D can be represented by S = exp (-D/Do). Further, Do is shown to depend on the time after exposure at which survival is assayed.

Biological Effects Induced by Non-thermal Ultrasound and Implications for Cancer Therapy

“…The effective range of OHradicals resulting from cavitation, as described above, is more likely to damage the cellular membrane than genetic material (42). This is supported by an earlier study showing that the effect of ultrasound using an ultrasound frequency of 65 kHz and intensities ranging from 0.1 to 2.24 W/cm 2 did not cause a significant increase in chromosome abnormalities (43). However, at an ultrasound frequency of 65 kHz, acoustic cavitation for the range of ultrasound intensities studied might not produce sufficient ROS to cause chromosomal abnormalities.…”

“…However, at an ultrasound frequency of 65 kHz, acoustic cavitation for the range of ultrasound intensities studied might not produce sufficient ROS to cause chromosomal abnormalities. In addition, Roseboro et al (43) employed a circulation system that transiently exposed the cell suspension to the ultrasound wave field. The circulating system contributed to disruption in cavitation formation and hence further decreased ROS formation.…”

Ultrasonic effects on alveolar macrophages in suspension

“…Finally, Roseboro et a1 (11) using lower frequency (65 kHz) than others cited here, observed survival reduction in human peripheral blood lymphocytes and HeLa cells exposed to one min of ultrasound at an acoustic power range of 0.12-2.24 W. A hollow cylindrical transducer was used, through which the cell suspension flowed. Thus, plasma membrane alterations in HeLa cells and thoracic duct lymphocytes (1 8) ; and endoplasmic reticulum dilation and plasma membrane alterations in human embryonic fibroblasts (19).…”