Effect of two models of stress associated with ligature-induced periodontitis on hematological parameters in rats

Abstract

Purpose: To evaluate the effect of two models of chronic stress in rats and their association with induced periodontitis on hematological parameters: mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH), mean corpuscular hemoglobin concentration (MCHC), hematocrit (Ht), erythrocytes (Hm), hemoglobin (Hg) and leukocytes (Lk). Methods: Forty-eight adult Wistar rats were randomly distributed into four groups (n=12): physical stress (PSG), variable stress (VSG), ligature (LG) and control (CG) and then started the test of physical stress (restraint and exposure to cold) and variable stress (exposure to flashing light, isolation, examination of the oral cavity, congested environment, the smell of blood and noise). After 10 days of the stress test, the animals in Groups PS, VS and L were anesthetized, and a silk thread was adapted around the upper right second molar; subsequently, the stress test continued for 50 days. The animals were anesthetized and held up the incision and visualization of the posterior vena cava for blood puncture vacuum in tubes containing EDTA. Data were collected by blinded and trained examiners and were statistically analyzed by means of ANOVA and Bonferroni's test at the significance level of 0.05. Results: The two models of stress changed all of the hematological parameters tested, with the exception of VCM. Conclusion: The stress associated with periodontitis is able to modify blood parameters in rats.

Effects ofSalvadora persicaExtract on the Hematological and Biochemical Alterations against Immobilization-Induced Rats

“…Similar to our studies, others have shown decreases in WBC and RBC counts in animals exposed to immobilization stress [ 18 ]. Some studies reported that immobilization changes in blood parameters such as Hb, HCT, MCV, MCH, and MCHC [ 19 ], but in this study no statistical differences were observed in these parameters. The increase in the hematological constituents in the stress loaded group cotreated with SP might be due to the antioxidant, antilipid peroxidation, and anticonvulsant effects of SP.…”

Erythrocyte indices and localized stage II/III periodontitis in military young men and women: CHIEF oral health study

“…Since women were more predisposed to iron-deficiency anemia than men, which might lead to a sex difference in the presentations of erythrocyte indices that women were more likely to have a lower hematocrit and hemoglobin level. On the other respect, those young adults with localized periodontitis and greater BMI in the present study might experience higher physical stress while on regular exercise training, which has been observed with an increase of erythrocyte indices in an animal model [ 37 ]. Based on these evidence mentioned above, we acknowledged that the relationships between erythrocyte indices and periodontitis should be interpreted carefully, which might vary by the stage of periodontitis, BMI and levels of physical activity or fitness of the study population.…”