Effect of Two Different Commercial DNA Extraction Kits on the Bacterial 16S Ribosomal RNA Gene Denaturing Gradient Gel Electrophoresis Profile of Arabian Gelding Feces

ZusammenfassungDie Mikrobiota im Dickdarm des Pferdes ist ein komplexes Gefüge, das durch die Fütterung stark beeinflusst werden kann. So führt beispielsweise die Fütterung eines stärkereichen Kraftfutters zum Anstieg von Bakterien, die Stärke fermentieren und Laktat produzieren, während sich die Anzahl der für die Faserverdauung zuständigen zellulolytischen Bakterien reduziert. Zur Optimierung der bakteriellen Mikrobiota werden vielfach Probiotika eingesetzt, um beispielsweise den potenziell negativen Einfluss stärkereicher Rationen zu minimieren. Für das Pferd sind in der Europäischen Union zurzeit lediglich verschiedene Stämme der Hefe Saccharomyces cerevisiae (SC) als Probiotikum zugelassen. In-vivo-Studien bei adulten Pferden konnten jedoch keine eindeutigen Effekte einer SC-Supplementation nachweisen.

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Beeinflussung der equinen Mikrobiota durch Fütterung und Probiotika: aktueller Stand der Forschung und rechtliche Grundlagen

Langner

Vervuert

2019

Tierarztl Prax Ausg G

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Two possible candidate enzymes from Ulva lactuca-associated epiphytic bacteria obtained through PCR and functional evaluation

2020

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Epiphytic bacteria from marine macroalgae synthesize enzymes of industrial and biotechnological interest. In this study, we obtained two DNA candidate fragments for lipid-degrading enzymes from the total DNA of Ulva lactuca-associated epiphytic bacteria. First, we evaluated a method for total bacterial DNA isolation from the surface of U. lactuca thalli. Then, we designed sets of primers and used them directly for PCR amplification. The resulting PCR products were sequence-analyzed and used for expression and functional evaluation with the Escherichia coli pBAD-TOPO system. We obtained high molecular weight and good quality total bacterial DNA that served as a template to identify a fragment corresponding to an Acetyl-CoA C-Acetyltransferase (or Thiolase), and a candidate fragment for a versatile “true” lipase. We expressed the possible “true” lipase gene fragment heterologously in Escherichia coli and obtained proof of hydrolytic activity on Tributyrin, Tween-20, and Olive-oil media. This study resulted in new knowledge on U. lactuca-associated epiphytic bacteria as possible brand-new sources of enzymes such as thiolases and “true” lipases. However, future studies are required to describe the characteristics and important applications of these candidate enzymes.

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Effect of Two Different Commercial DNA Extraction Kits on the Bacterial 16S Ribosomal RNA Gene Denaturing Gradient Gel Electrophoresis Profile of Arabian Gelding Feces

Cited by 2 publications

References 39 publications

Beeinflussung der equinen Mikrobiota durch Fütterung und Probiotika: aktueller Stand der Forschung und rechtliche Grundlagen

Beeinflussung der equinen Mikrobiota durch Fütterung und Probiotika: aktueller Stand der Forschung und rechtliche Grundlagen

Two possible candidate enzymes from Ulva lactuca-associated epiphytic bacteria obtained through PCR and functional evaluation

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