Effect of tumour necrosis factor on cultured human melanoma cells

Helson, Lawrence; Green, Saul; Carswell, Elizabeth A.; Old, Lloyd J.

doi:10.1038/258731a0

Cited by 218 publications

(72 citation statements)

References 2 publications

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“…TNF␣ is a pro-inflammatory cytokine that has a variety of antitumor activities. It is cytotoxic to tumor cells, 16,17 enhances expression of HLA antigens 18 and ICAM-1 19 on tumor cells, chemo-sensitizes, [20][21][22] and radio-sensitizes tumor cells 23 and can be lytic to the tumor vasculature. 24,25 The major problem associated with utilizing TNF as an anti-cancer therapy has been its systemic toxicities.…”

Section: Figure 1 Diagram Of Ad5 E3 Region Depicting Transgene Insertmentioning

confidence: 99%

Gene delivery from the E3 region of replicating human adenovirus: evaluation of the E3B region

Hawkins

Hermiston²

2001

Gene Ther

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Section: Figure 1 Diagram Of Ad5 E3 Region Depicting Transgene Insertmentioning

confidence: 99%

Gene delivery from the E3 region of replicating human adenovirus: evaluation of the E3B region

Hawkins

Hermiston²

2001

Gene Ther

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“…Inhibition of TNF-mediated cytotoxicity was evident in both the presence and absence of actinomycin D or cycloheximide. 3. TNF itself was not found to be a protease, as it had no proteolytic activity in a sensitive colorimetric assay.…”

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confidence: 99%

Involvement of a serine protease in tumour‐necrosis‐factor‐mediated cytotoxicity

Suffys

Beyaert

Roy

et al. 1988

European Journal of Biochemistry

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We investigated the effect of various protease inhibitors on the anti-proliferative and cytotoxic action of tumour necrosis factor (TNF) on mouse L929 fibrosarcoma cells.1. The following serine-type protease inhibitors led to inhibition of TNF action: phenylmethylsulfonyl fluoride, N"-p-tosyl-L-lysine chloromethane, Nu-p-tosyl-L-phenylalanylchloromethane, Na-p-tosyl-L-arginine methyl ester, L-leucine methyl ester, DL-phenylalanine methyl ester, N-acetyl-DL-phenylalanine-8-naphthyl ester, p-nitrophenyl p'-guanidino-benzoate and antipain. We could not detect an effect of inhibitors specific for thiol protease on TNF.2. Inhibition of TNF-mediated cytotoxicity was evident in both the presence and absence of actinomycin D or cycloheximide.

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“…TNF-a alters cellular growth, differentiation, and metabolism (3)(4)(5)(6)(7). Of special interest are its cytostatic and cytotoxic actions on tumor cell lines in culture (1,(3)(4)(5)8) and its ability to cause hemorrhagic necrosis and regression of tumors in experimental animals (1,8).…”

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confidence: 99%

Tumor necrosis factor induces phosphorylation of a 28-kDa mRNA cap-binding protein in human cervical carcinoma cells.

Marino

Pfeffer

Guidon

et al. 1989

Proc. Natl. Acad. Sci. U.S.A.

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Tumor necrosis factor a (TNF-a) stimulated the phosphorylation of a 28-kDa protein (p28) in the ME-180 line of human cervical carcinoma cells. The effect of TNF-a on the phosphorylation state of p28 was rapid (4-fold increase within 15 min) and persistent, remaining above the basal level for at least 2 hr. The specific binding of 12'I-labeled TNF-a to cell-surface binding sites, the stimulation of p28 phosphorylation by TNF-a, and the inhibition of cell proliferation by TNF-a occurred with nearly identical dose-response relationships. Two-dimensional SDS/PAGE resolved p28 into two isoforms having pI values of 6.2 and 6

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Effect of tumour necrosis factor on cultured human melanoma cells

Cited by 218 publications

References 2 publications

Gene delivery from the E3 region of replicating human adenovirus: evaluation of the E3B region

Gene delivery from the E3 region of replicating human adenovirus: evaluation of the E3B region

Involvement of a serine protease in tumour‐necrosis‐factor‐mediated cytotoxicity

Tumor necrosis factor induces phosphorylation of a 28-kDa mRNA cap-binding protein in human cervical carcinoma cells.

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