Effect of Tumor Necrosis Factor‐α on the Intracellular <scp><scp>Ca</scp><sup>2+</sup></scp> Homeostasis in Human Sperm

Carrasquel, Gabriela; Camejo, María Isabel; Michelangeli, Fabián A.; Ruiz, Marie Christine

doi:10.1111/aji.12106

Cited by 7 publications

(6 citation statements)

References 46 publications

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“…As shown in the above, the effect induced by IFN-g on Ca 2+ permeability, was completely prevented in the presence of the antioxidant BHT. Similar results were found in human sperm using high concentration of TNF-a [Carrasquel et al 2013]. These results suggest that IFN-g oxidatively damages sperm membranes affecting the stability of some of the proteins that regulate Ca 2+ transport.…”

Section: Discussionsupporting

confidence: 80%

“…However, a previous study demonstrated that IFN-g markedly reduced the activities of Na + -K + -ATPase, Ca 2+ -ATPase in sperm and decreased the rate of acrosome reaction [Bian et al 2007]. Additionally, we had previously observed that TNF-a (another cytokine), decreases membrane permeability to Ca 2+ and affects Ca 2+ regulation in sperm cells in vitro [Carrasquel et al 2013]. …”

Section: Discussionmentioning

confidence: 95%

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IFN-gamma alters the human sperm membrane permeability to Ca²⁺

Carrasquel

Camejo

Michelangeli

et al. 2013

Systems Biology in Reproductive Medicine

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Inflammation in the male genitourinary tract has been associated with the release of pro-inflammatory cytokines such as interferon-gamma (IFN-γ) and elevated reactive oxygen species, which affects spermatozoa capacitation, motility, and the acrosome reaction, along with functions regulated by the concentration of cytoplasmic Ca(2+) ([Ca(2+)]cyto). Though Ca(2+) signaling is of particular significance in sperm, the effect of IFN-γ intracellular calcium on these cells is still unknown. The present study evaluated the effect of IFN-γ on the [Ca(2+)]cyto and Ca(2+) permeability on human sperm. A cell suspension loaded with fura-2 was incubated with or without IFN-γ (from 0 to 2000 pg/ml) for 0, 30, 60, and 120 minutes, and the [Ca(2+)]cyto was measured. The permeability to Ca(2+) was evaluated by the change of the intracellular concentration following an extracellular Ca(2+) pulse. IFN-γ at low concentrations (≤ 500 pg/ml) did not affect the [Ca(2+)]cyto and Ca(2+) permeability of sperm. At a high concentration (2000 pg/ml), IFN-γ did not alter the [Ca(2+)](cyto), but significantly decreased the magnitude and velocity of Ca(2+) entry into the cell. This effect was dependent on incubation time and IFN-γ concentration. This alteration induced by IFN-γ was prevented by the simultaneous incubation of sperm with the antioxidant butylhydroxytoluene (BHT). In conclusion, in vitro, IFN-γ modifies Ca(2+) sperm membrane permeability, probably via lipid peroxidation. IFN-γ in high concentration, as observed in inflammation/infection, can affect [Ca(2+)](cyto) regulation and alter sperm fertilizing capacity.

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Section: Discussionsupporting

confidence: 80%

Section: Discussionmentioning

confidence: 95%

IFN-gamma alters the human sperm membrane permeability to Ca²⁺

Carrasquel

Camejo

Michelangeli

et al. 2013

Systems Biology in Reproductive Medicine

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“…In the testis, most of the germ cells undergo spontaneous degeneration during spermatogenesis and TNF-α determines the size of the germ cell population in the seminiferous epithelium by inducing germ cell apoptosis and disrupting Sertoli cell junctions as well as inhibiting steroidogenesis in Leydig cells (Lysiak, 2004 ). The high concentration TNF-α can alter intracellular Ca 2+ homeostasis by decreasing plasma membrane permeability to Ca 2+ (Carrasquel et al, 2013 ), whereas Ca 2+ signaling mediated responses are one of the main factors regulating sperm function (Publicover et al, 2007 ). Many studies reported that Ca 2+ signaling is involved in the regulation of dynein activity in mice sperm flagella (Lesich et al, 2012 ).…”

Section: Discussionmentioning

confidence: 99%

Obesity or Overweight, a Chronic Inflammatory Status in Male Reproductive System, Leads to Mice and Human Subfertility

Fan

Liu

et al. 2018

Front. Physiol.

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Obesity is frequently accompanied with chronic inflammation over the whole body and is always associated with symptoms that include those arising from metabolic and vascular alterations. On the other hand, the chronic inflammatory status in the male genital tract may directly impair spermatogenesis and is even associated with male subfertility. However, it is still unclear if the chronic inflammation induced by obesity damages spermatogenesis in the male genital tract. To address this question, we used a high fat diet (HFD) induced obese mouse model and recruited obese patients from the clinic. We detected increased levels of tumor necrosis factor (TNF-α), interleukin-6 (IL-6), and NOD-like receptor family pyrin domain containing-3 (NLRP3) in genital tract tissues including testis, epididymis, seminal vesicle, prostate, and serum from obese mice. Meanwhile, the levels of immunoglobulin G (IgG) and corticosterone were significantly higher than those in the control group in serum. Moreover, signal factors regulated by TNF-α, i.e., p38, nuclear factor-κB (NF-κB), Jun N-terminal kinase (JNK), extracellular signal-regulated kinase (ERK), and their phosphorylated status, and inflammasome protein NLRP3 were expressed at higher levels in the testis. For overweight and obese male patients, the increased levels of TNF-α and IL-6 were also observed in their seminal plasma. Furthermore, there was a positive correlation between the TNF-α and IL-6 levels and BMI whereas they were inversely correlated with the sperm concentration and motility. In conclusion, impairment of male fertility may stem from a chronic inflammatory status in the male genital tract of obese individuals.

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“…Numerous studies have proved that high cytokine levels may determine an alteration of sperm characteristics. [18][19][20][21] In our case, for the first time, we have detected increased amounts of proinflammatory cytokines directly in semen samples during infections by CagApositive H pylori strains, which could realistically be responsible for the reduced sperm quality observed in infected individuals. The proposed mechanism is supported by the observation that both cytokines showed significant a positive correlation with the percentage of necrotic sperm.…”

Section: Commentmentioning

confidence: 54%

Sperm Parameters and Semen Levels of Inflammatory Cytokines in Helicobacter pylori–infected Men

Moretti

Figura

Campagna

et al. 2015

Urology

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Effect of Tumor Necrosis Factor‐α on the Intracellular Ca²⁺ Homeostasis in Human Sperm

Abstract: Tumor necrosis factor-α decreases membrane permeability to Ca(2+) and affects Ca(2+) regulation in sperm cells in vitro, probably via lipid peroxidation, which may explain the decrease in sperm fertilizing capacity during inflammatory and infectious processes.

Cited by 7 publications

References 46 publications

IFN-gamma alters the human sperm membrane permeability to Ca²⁺

IFN-gamma alters the human sperm membrane permeability to Ca²⁺

Obesity or Overweight, a Chronic Inflammatory Status in Male Reproductive System, Leads to Mice and Human Subfertility

Sperm Parameters and Semen Levels of Inflammatory Cytokines in Helicobacter pylori–infected Men

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Effect of Tumor Necrosis Factor‐α on the Intracellular Ca2+ Homeostasis in Human Sperm

Abstract: Tumor necrosis factor-α decreases membrane permeability to Ca(2+) and affects Ca(2+) regulation in sperm cells in vitro, probably via lipid peroxidation, which may explain the decrease in sperm fertilizing capacity during inflammatory and infectious processes.

Cited by 7 publications

References 46 publications

IFN-gamma alters the human sperm membrane permeability to Ca2+

IFN-gamma alters the human sperm membrane permeability to Ca2+

Obesity or Overweight, a Chronic Inflammatory Status in Male Reproductive System, Leads to Mice and Human Subfertility

Sperm Parameters and Semen Levels of Inflammatory Cytokines in Helicobacter pylori–infected Men

Contact Info

Product

Resources

About

Effect of Tumor Necrosis Factor‐α on the Intracellular Ca²⁺ Homeostasis in Human Sperm

IFN-gamma alters the human sperm membrane permeability to Ca²⁺

IFN-gamma alters the human sperm membrane permeability to Ca²⁺